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The experiment result showed: when the medium was MS supplemented with 2,4-D2.0mg/L, BAl.0mg/L and NAA0.5mg/L, the callus induction rate of the cotyledon was 87.9%, the callus induction rate of the plumular axis was 82.9%, and the callus induction rate of the terminal bud was 6.7%.

实验结果表明在MS附加2,4-D2.0mg/L、BA1.0mg/L、NAA0.5mg/L的培养基上,子叶愈伤组织的诱导率为87.9%、胚轴的诱导率为82.9%、顶芽的诱导率为6.7%。

Preinduction was performed using basic fibroblast growth factor, which could promote the differentiation from BMSCs into nerve precursor cells or neurons. The expression of neuroprotein molecule was coincided with the development of neural cells.

在添加化学诱导剂之前先使用成纤维生长因子进行预诱导,能促进骨髓间充质干细胞向神经前体细胞和神经元转化,且在诱导分化过程中神经蛋白分子的表达顺序与神经细胞发育过程一致。

RESULTS: After 24 hours of preinduction, cells changed from spindle to polygonal or irregular, with many short rod-shape processes and 2-3 nucleoli. Whirlpool shape growth disappeared, and was disordered. After 1-3 hours of induction, cells gradually became round, with significant cell contraction, strong refraction, bipolar or pluripolar processes connected each other into a net. After 5 hours of induction, grades one and two branches were detected.

结果:预诱导24 h,胞体由原来的长梭形变成多角形或不规则形,伸出多个短棒状突起,可见2~3个核仁,细胞间漩涡状生长趋势消失,排列无规律;诱导1~3 h,细胞逐渐变圆,胞质收缩明显,折光性增强,双级或多极的突起互相连接呈网状;诱导5 h,突起出现一、二级分支。

The trial results show that with the disposal of induced caving roof by presplitting blasting and induced caving roof blasting, about 17.8 m in thickness of rock cushion is successfully formed in the inferior abandoned stope, which can bring down the harm of dashing blast of an explosion caused by roof collapse; apparent run-through flaws arise between presplitting blasting holes, and controllable caving is formed in the roof, abrupt collapse of large area roof is prevented, the productional safe surroundings of underground mining is improved; eventually, the resources' reclamation rate is improved from about 70% to 84.6%, and the dilution rate is abated from 25%40% to below 15%, so efficiently withdrawing is actualized.

试验结果表明:采用预裂爆破与诱导崩顶爆破的方法,通过对顶板的诱导崩落处理,成功地在下方采空区形成了厚度为17.8 m左右的岩石缓冲垫层,有效地降低顶板冒落带来的冲击气浪危害;在预裂孔间产生明显的贯通裂隙,从而在顶板上形成诱导可控崩落,防止顶板大面积的突然垮落,改善地下开采安全生产环境;最终资源的回收率由70%左右提高到84.6%,贫化率由25%~40%降低到15%以下,实现了资源的高效回收。

Result The optimum medium formula for protocorm propagation in tissue culture of C. grandiflorium was MS+0.1 mg/L 6-BA. The suitable medium for protocorm induction and differentiation in tissue culture of C. grandiflorum was WS+1.5 mg/L 6-BA+0.3 mg/L NAA with the bud-inducing rate up to 130%. The suitable medium for root inducement was 1/2MS+0.2 mg/L NAA+1.0 mg/6-BA and the survival rate of in vitro plantlets after transplanting was 78%.

结果]大花蕙兰组织培养原球茎粉殖以MS+0.1 mg/L NAA+2.0 mg/L6-BA培养基配方最好;适合大花蕙兰组织培养原球茎诱导分化的培养基配方为MS+1.5 mg/L6-BA+0.3 mg/L NAA,诱导出芽率可达130%;适合大花蕙兰诱导生根的培养基配方为1/2 MS+0.2 mg/L NAA+1.0 mg/L6-BA,试管苗移栽后成活率为78%。

The germination test of Sporocarps of Microlepia hancei prantl had been done, the callus can be induced from the delicate stem of Microlepia hancei prantl; The inducement and proliferation medium of callus was MS+6-BA2 mg/L+NAA0.2 mg/L;the optimal combination of hormones of buds differentiation was MS+6-BA 0.4 mg/L; the effect of MS medium in radication culture was better than 1/2MS, the roots of plants were all efficiently induced in MS medium without reference to adding IBA、NAA or IBA+NAA, the rate of radication was high; the effect of seedling cultured in the mixture of perlite and turf soil was better than perlite、garden soil and river sands.the mixture of perlite and turf soil has the excellences such as ventilate、water conservation 、nourishment and so on,the survival rate was higher and seedling substance was also hale.

试验进行了华南鳞盖蕨孢子果的萌发试验,并利用幼嫩茎段进行诱导愈伤组织;愈伤组织的诱导与增殖培养基为MS+6-BA2 mg/L+NAA0.2 mg/L;其芽分化最佳激素组合为MS+6-BA 0.4 mg/L;生根培养中MS基本培养基比1/2MS效果好,MS基本培养基不论附加生长素IBA、NAA或IBA+NAA,均能有效地诱导植株生根,生根率高;假植以珍珠岩和泥炭土的混合物为基质效果优于珍珠岩、菜园土和河沙,具有通气、保水、营养等优点,假植成活率高,苗势健壮。

Roentgenographic and histologic observation confirmed that bBMP had efficient bone morphogenetic activity.

论文第一部分参照杨连甲的方法从小牛骨基质中提取bBMP,经SDS-PAGE电泳分析,bBMP保留有BMP活性蛋白成分,植入小鼠股部肌肉后的X线及组织学观察发现,所提取的bBMP具有较高的骨诱导活性,诱导成骨的时间和过程与以往的报道一致。bBMP诱导骨形成过程呈现连续又具显著的阶段性,本研究结合组织学和组织化学观察的特点,将其全过程分为四个时期。

Our results indicate that 1 NSC606985, at nanomolar level, can effectively induce apoptosis in AML cells NB4 and U937 and significantly inhibit the proliferation without cell death in breakpoint cluster region–Abelson murine leukemia kinase-carrying leukemic K562 cells; 2 At such low concentrations, this agent also significantly inhibits the clonogenic activity of hematopoietic progenitors from patients with AML; 3 For apoptosis induction, NSC606985 rapidly induces the proteolytic activation of protein kinase Cδ with loss of mitochondrial transmembrane potential and caspase-3 activation; 4 Co-treatment with rottlerin, a PKCδ-specific inhibitor, completely blocks NSC606985-induced mitochondrial m loss and caspase-3 activation, while the

结果显示:(1)纳摩尔浓度的NSC606985即可有效诱导AML细胞系NB4和U937细胞凋亡并显著抑制含有bcr-abl融合蛋白激酶的K562细胞的增殖;(2)低浓度的NSC606985也显著抑制来自AML病人骨髓的新鲜白血病细胞的克隆形成能力;(3)除了迅速诱导线粒体跨膜电位的崩塌及Caspase-3的活化外,NSC60698也导致蛋白激酶Cδ的水解激活;(4)PKCδ特异的抑制剂rottlerin能够完全抑制NSC606985诱导的线粒体跨膜电位的崩塌和Caspase-3的活化,而Caspase-3特异的抑制剂z-DEVD-fmk仅能部分削弱PKCδ的活化及细胞的凋亡;(5)以移植PML-RARα转基因小鼠产生的白血病细胞建立的模型研究了该化合物对白血病可能的治疗作用。

Carmichaeli leaves were studied after spraying salicylic acid 2 days before inoculation with Septoria aconite Bacc.

用5mmol/L的水杨酸对附子抗病品种和感病品种分别进行诱导,两天后接种乌头壳针孢,研究处理后附子叶片相关酶活性的变化,结果表明,SA诱导后能明显增强附子叶片内POD、PPO、PAL的活性;诱导接种处理比对照接种处理酶活性增加更为明显,同时抗病品种比感病品种酶活性更强,增加幅度更大,高酶活持续时间更长。

The results showed that both sporophores and stromata of Cordyceps militaris had high content of polysaccharide. And polysaccharide content in stromata was 2-4 times higher than that in sporophores. CMPS could decrease the AST, ALT and MDA levels, and restore GSH-Px content and cell viability remarkably in supernatant of cultured hepatocytes injured by CCl4 induction.

结果表明:蛹虫草的子实体和基质都含有丰富的多糖,其中基质中的多糖含量最高,约为子实体的2~4倍;CMPS可明显降低由CCl4诱导的损伤肝细胞培养上清液中谷草转氨酶、谷丙转氨酶的活性水平和丙二醛的含量水平,显著提高由CCl4诱导的损伤肝细胞培养上清液中谷胱甘肽过氧化物酶活性和诱导损伤肝细胞的存活率。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?