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Objective To compare influence of several biotic elicitors (yeast extract, oligogalacturonides, and fungal elicitor) and abiotic elicitors (Ag^+, Co^2+, and a-amino isobutyric acid) on tanshinone production in the hairy root culture of Salvia miltiorrhiza.

目的比较几种生物诱导子酵母提取物、寡半乳糖醛酸、真菌诱导子3和非生物诱导子(Ag^+、Co^2+和α-氨基异丁酸)对丹参毛状根培养生产丹参酮的影响。

The time to losing eyelash reflex and intubation were recorded .The incidence of cough ,apnoea ,and laryngospasm during the induction period were recorded too. The variety of MAP、HR、SpO2% were recorded in the every time point of induction. The data was analyzed with SPSS software.

记录两组从诱导开始至意识消失时间,插管时间,有无插管反应(呛咳、屏气、喉痉挛及支气管痉挛),记录诱导前、诱导后、插管即时和插管后2min各时间点的HR、 SpO2%及MAP变化,用方差分析和t检验进行统计学的分析。

Bone marrow mesenchymal stem cells could differentiate into osteoblasts, adipocytes and neural like cells with osteoblast inductor (β-sodium glycerophosphate, dexamethasone, vitamin C), lipoblast inductor (dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, indometacin) and serum-free medium inductor (dimethyl sulphoxide, butylated hydroxyanisole) respectively. Osteoblast marker (alkaline phosphatase, osteocalcin mRNA, calcium node), adipocyte marker (lipid droplet, PPAR γ-2mRNA) and neural cell-like marker (nissl body, neuron specific enolase, neurofilament protein) were respectively determined by the immunohistochemical method, polymerase chain reaction and immunocytochemical method.

分别采用成骨细胞诱导剂(β-甘油磷酸钠,地塞米松,维生素C)、成脂肪细胞诱导液(地塞米松,甲基异丁酸黄嘌呤,牛胰岛素,吲哚美辛)及二甲基亚砜和羟基丁酸苯甲醚无血清培养基诱导剂干预细胞向成骨、脂肪、神经细胞分化,经免疫组织化学染色、PCR、免疫细胞染色方法检测成骨标志物(碱性磷酸酶、骨钙素mRNA、钙结节)、脂肪标志物(脂滴、PPARγ-2mRNA)、以及类神经标志物(尼克氏体、神经烯醇化酶、神经丝蛋白)。

In the pre-experiment, we haved induced MSC to osteogenic cells adopting different kinds of inducers singly and mixedly. It indicates that the induction method of mixed inducers of BMP〓100ng/ml, bFGF 2ng/ml, VitC 50ug/ml and Dexamethasone 10nmol/L is more effective and economical.

我们在预实验中,曾采用多种诱导因子单独或不同组合方式对MSC进行骨起源诱导,结果表明:〓100ng/ml、bFGF2ng/ml、维生素C50ug/ml、地塞米松10nmol/L组成的多种因子复合诱导方式最为经济有效。

In SCD survivors, sustained monomorphic ventricular tachycardia is inducible by electrophysiologic testing in 40 to 50% and polymorphic VT in 10 to 20%; in 30 to 50%,no sustained arryhthmia is induced.

在心源性猝死生还者中, 40~50%电生理试验能诱导持续单一型室性心动过速,10~20%能诱导多型的,30~50%不能诱导持续的节律异常。

The growth and survival of Mytilus galloprovincialis post-larvae metamorphosed using different chemical compounds were investigated in this study to identify potential inducers in aquaculture and antifouling and biofouling researches.

中文摘要:在以往实验的基础上,跟踪调查了筛选出的化学诱导物质对紫贻贝幼虫变态后的生长和成活率的影响,并比较了其与相同条件下自然诱导物、微生物膜诱导后的稚贝的生长和成活率。

METHODS: mononuclear cells were isolated from axillary draining lymph node, and the adherent cells were cultured with rhGMCSF and rhIL4 to induce DC. The nonadherent cells were cultured with IL2 to induce tumor draining lymph node cells.

以多种细胞因子联合诱导腋下淋巴结单个核细胞中的贴壁细胞为DC,非贴壁细胞与IL2共培养后诱导成为肿瘤区域引流淋巴结细胞,用自体乳腺癌细胞冻融抗原刺激DC,并和TDLNC共培养,以诱导肿瘤抗原特异性CTL。

METHODS: The mononuclear cells were isolated from axillary draining lymph node, and the adherent cells were cultured with rhGMCSF and rhIL4 to induce DC. The nonadherent cells were cultured with IL2 to induce tumor draining lymph node cells.

以多种细胞因子联合诱导腋下淋巴结单个核细胞中的贴壁细胞为DC,非贴壁细胞与IL2共培养后诱导成为肿瘤区域引流淋巴结细胞,用自体乳腺癌细胞冻融抗原刺激DC,并和TDLNC共培养,以诱导肿瘤抗原特异性CTL。

Based on these results and inferred to related reports from other labratories, it was possible to make some analyses and conclusions or inferrences:(1) CD〓AK with tumoricidal activity were induced and expanded in number througth costimulation of PBMC with anti-CD〓 McAb . and r IL-2 ;(2) CD〓AK induced and expanded in such manner did exibit more potent proliferation·ability and cytotoxicity which maintained for lonser time than those of LAK cells, thus CD〓AK was a new variety of antitumor effector cells worth to be explored;(3) CD〓AK could mediate MHC nonrestricted cytotoxicity and kill tumor target cells through inducing necrosis and apoptosis;(4) Normal mature lymphocytes of PBMC could be induced to proliferate and /or to die from apoptosis when they were costimulated by anti-CD〓McAb and rIL-2. Both proliferation and apoptosis were existing in the same cultivation system sugsesting that the presence of rIL- 2 might provide some accessary signals for apoptosis.

以这些结果为基础并参考其它有关文献可能做出如下分析与结论或推论:(1)用抗CD〓单抗和rIL-2共刺激外周血单个核细胞能诱生扩增出具有杀瘤活性的CD〓AK细胞,(2)与LAK相比,用这种方法诱生扩增的CD〓AK增殖能力强、细胞毒活性强而且维持时间长;CD〓AK是一类值得开发的抗瘤效应细胞;(3)CD〓AK能够介导MHC非限制性细胞毒活性,可以通过诱导靶细胞坏死和/或凋亡杀伤肿瘤细胞;(4)正常外周血单个核细胞中的成熟淋巴细胞在受到抗CD〓单抗和rIL-2共同刺激后既可诱导增殖也可诱导凋亡,两者并存于同一体系,推测rIL-2的存在可能为细胞凋亡提供一些辅助信号。

Cell growth curve was also drawed before and after differentiation. When cultured in the osteoplastic induction medium, ADSCs derived epithelial cells might further differentiate into osteoblasts.

在成骨细胞诱导培养基中进一步诱导ADSCs源表皮细胞向成骨细胞定向分化,对诱导后的细胞进行ALP染色和Yon Kossa染色检查。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?