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To study the efficacity of space-induced towards the Artemia sinica spawn and select individuals with big genetic differences to propagate,this paper collect totally 59 space-induced and control Artemia sinic to carry out the genetic diversity inspection and relative's analyses using four sets of AFLP selective primers for amplification.

每对引物均获得清晰的扩增图谱,共检出222个清晰可辨的不同扩增片段,其中多态位点189个,占85.1%;UPGMA和NJ聚类分析图显示,来自空间诱变组的M5单独聚类,而其它58个个体聚为另一类,其他太空诱变组与地面对照组的个体交叉聚类,地面对照和太空诱变组遗传差异不明显。

Condusion:The method of compound mutagenesis of HNO_2 and UV of protoplast for screening of...

亚硝酸与紫外线复合诱变原生质体是一种很好的诱变方式。

The results showed that while the raying distance was 10 cm and an ultraviolet lamp with 20 W acted on the strain of Norcardia corailina 3 min and 4 min, the lethal ratio was high than 90%; While the raying time was 5 min, the lethal ratio was 100%.

结果表明:在紫外灯功率为20w,照射距离为10cm的条件下,诱变时间为3min和4min,致死率均大于90%;而诱变时间为5min,致死率为100%。

The optimum concerntration of EMS was studied for mutagenesis of salt tolerance under another callus and leaf of strawberry in vitro culture.

河北农业大学;河北省林业局;摘要:以全明星草莓花药诱导的愈伤组织以及组培苗叶片为试验材料,用化学诱变剂甲基磺酸乙酯进行诱变处理。

In this paper, an efficient and easy biological screening method based on the sensitivity to lovastatin of Neurospora crassa was built to deal with the mutant strain rudely by UV mutation. A high-level strain was screened in the shaking flask culture incorporating with TLC and HPLC. A comparison between this two methods also was originally discussed while identifying dyeing using bromophenol blue as the better treatment of TLC, and offering a kind of simple, practical, fast and economical determination qualitatively and quantitatively.

本文有鉴于此,通过紫外诱变处理,并基于粗糙脉孢酶对洛伐他汀的生物敏感性,对诱变菌株进行了初筛;同时,采用摇瓶培养,结合TLC和HPLC的分析方法,复筛出一株高产菌株;对复筛过程两种分析方法分别进行了初步探讨,比较其优劣,确定TLC法经溴酚兰显色剂显色处理为较佳方式;从而,建立了一套简单、实用、快速、经济且有效的洛伐他汀生产菌诱变筛选方法。

The method of mutation breeding and the current research situation of Thiobacillus ferrous oxide are summarized,and some problems in the process of breeding are discussed.

综述了氧化亚铁硫杆菌诱变育种的方法以及诱变育种的研究现状,并对诱变育种过程中的一些问题进行了探讨。

To culture antibiotic overproducing strains, the study include using different physical and chemical factors to induce Streptomyces thermocarbonxydus var shandaensis to mutation. After original strain were mutagensised by UV combined LiCl、~(60)Co、 DES, 9 high-yield strains were obtained.

通过紫外线加氯化锂复合诱变得到高产菌株UV—18、UV—19、UV—20,其中菌株UV—19的发酵上清液抑菌圈直径是原种的1.875倍;通过~(60)Co诱变得到高产菌株Co—22、Co—36、Co—38,其中菌株Co—38的发酵上清液抑菌圈直径是原种的1.68倍;通过硫酸二乙酯诱变得到高产菌株DES—21、DES—31、DES—37,其中菌株DES—21的发酵上清液是原种的1.52倍。

When treatment leaves in vitro with EMS only, two different ways were applied as bellow:① Treatment with mutagen solution.

在化学诱变剂EMS对离体叶片的处理中,EMS溶液浸泡处理的诱变效果好于EMS液体培养的诱变效果。

When treatment on the leaves in vitro of strawberry cultivars of All Star and Xinminxing by any mutagens, the result showed that the survival and regeneration rates have no difference .

对诱变剂的敏感性 '全明星'草莓和'新明星'草莓的离体叶片在各种诱变剂的处理下,成活率和再生率之间没有显著差异,表明两个品种对各种诱变剂的敏感性相似。

"The effects of some mutagens are increased or suppressed in some organisms by the presence of certain other, nonmutagenic substances; oxygen, for example, makes cells more sensitive to the mutagenic effects of X rays."

有机体内某些非诱变物质的存在,可以加强或抑制一些诱变因子的作用,例如氧可使细胞对X光的诱变作用更加敏感。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

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不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。