诱变的

In this paper, the advances in research of laser mutation breeding mechanism was expounded and the application and achievements in breeding of agriculture, pasturage, farrier and industry microorganism of laser mutation were introduced.

阐述了激光诱变育种机理的研究进展，介绍了激光诱变在农作物、畜牧兽医、工业微生物育种上的应用及取得的成果，并介绍了激光诱变育种的一般操作过程，最后展望了激光诱变育种今后的研究前景。

As shown by the result, the biomass and astaxanthin content of oceanic red yeast was reduced due to the presence of β-ionone which inhibited the synthesis of carotenoids. The lethality rate of oceanic red yeast would attained 92.3% if 370 mg/L of the β-ionone concentration was contained in the plate medium. The oceanic red yeast mutated by ethyl methane sulfonate was cultured on plate medium containing 370 mg/L of the β-ionone. 200 mutants were randomly screened, positive mutants with increased the biomass, astaxanthin volumetric yield and astaxanthin specific yield accounted for 18% of the total strain, the mutants with higher biomass, astaxanthin volumetric yield and astaxanthin specific yield higher occupied 22.5%, 45% and 46%, respectively.

实验结果表明，在β-紫罗兰酮存在的情况下，由于类胡萝卜素合成受到抑制，海洋红酵母的生物量和虾青素合成量都减少；当平板培养基中β-紫罗兰酮浓度达到370 mg/L时，海洋红酵母的致死率为92.3%；在这种平板培养基上涂布经甲基磺酸乙酯诱变的海洋红酵母，随机筛选200个菌落，结果表明生物量、虾青素体积产率和细胞产率均有所提高的正突变株占18%，生物量、虾青素体积产率和细胞产率的单项指标有所提高的正突变株分别占22.5%、45%和46%。

Pingyangmycin as、a mutagen was applied to crop mutational breeding in this experiment. The mutagenic effects of PYM on barley was studied by means of seed treatment.

本试验以平阳霉素为诱变剂处理大麦种子，研究了该诱变剂对大麦的诱变效应及其适宜的浓度。

Mutation breeding of Phanerochaete chrysosporium by UV-light and nitrite was investigated, and the capability of decolorization to Safranine T and dye of high concentration after induced mutation was tested..

通过紫外线照射和亚硝酸盐两种诱变手段对黄孢原毛平革菌进行了诱变育种实验，进而考察了诱变后筛选出的优势菌对高浓度染料、番红花红的降解情况，并以此对比评价了两种诱变方法的诱变效果。

In the present experiment, the screening techniques for salt tolerance and mutants of strawberry leaf and anther callus in vitro were studied, and primary screening system for salt tolerance evaluation was established. It was studied the sensitivity to mutagen of different strawberry cultivars and different tissues in vitro.

本试验以草莓离体叶片及花药愈伤组织为试材进行了耐盐突变体的诱变及筛选研究，初步建立了草莓耐盐突变体诱变筛选的技术体系，研究了草莓品种以及离体组织对诱变剂的敏感性，并且对不同诱变处理再生植株的耐盐性差异进行了比较。

Studies on the mutant breeding of high-yield gibberellins strain The protoplast of Gibberella fujikuroi m978 were treated by mutation breeding forhigh-yield gibberellins, LiCl mutation was better than DES mutation or UV irradiation ifsingle mutation, but any single mutation was worse than compound mutation by whichthe mutants were more overproducting and genetically stable.

采用传统诱变育种，筛选高产菌株在原生质体条件下，对赤霉素产生菌m978的原生质体进行菌种选育；使用单因子诱变方法，LiCl处理的效果优于DES诱变和UV诱变；复合诱变比单因子诱变更容易得到高产突变株，且获得的高产突变株稳定性更优。

The mutagen amount used is 1%, when the mutafacient time is 50 minutes, this strain lethality rate is 75%.

以其作为出发株，得出了以硫酸二乙酯诱变该株的致死率曲线，诱变剂用量为1%、诱变时间为50min时，该株的致死率达到75%。

In this study, as a original strain, antagonistic bacterium Ata28 was mutated by UV, UV+LiCl, DES and Microwave irradiation; the very goal of the research is to improve the control efficiency of Ata28 strain against tobacco brown spot and obtain strains advanced both in antagonism and biological characteristics, further provides theoretics and practical experience to the production.

采用紫外线和氯化锂复合诱变Ata28菌株，通过预备实验，计算Ata28菌株在不同诱变时间的致死率和正变率，选择该菌株的最佳诱变时间10s，15s诱变；通过平板对峙培养，筛选出8个突变菌株在PDA平板上可明显抑制Tbs3的生长，抑菌带高于出发菌株Ata28，抑菌带最大值达9.7mm；经温室控病初筛和复筛试验，获得了对烟草赤星病有较好防效的突变菌株Uv1-190、Uv1-199，烟苗经Uv1-190、Uv1-199菌液处理3d后，接种Tbs3的防治效果达51.3％、54.9％，比出发菌株Ata28的防治效果好。

Ata28 strain exhibited marked suppression of Tbs3 strain which is the pathogen of tobacco brown spot on PDA plates, and the Ata28 strain had the good control efficiency of tobacco brown spot.

采用紫外线诱变Ata28菌株时，通过预备实验，计算Ata28菌株在不同诱变时间的致死率和正变率，选择该菌株的最佳诱变时间10s，20s，30s诱变；通过平板对峙培养，筛选出15个突变菌株在PDA平板上可明显抑制Tos3的生长，抑菌带高于出发菌株Ata28，抑菌带最大值达9.4 mm；在温室控病中，获得了对烟草赤星病有较好防效的突变菌株Uv-126，烟苗经Uv-126菌液处理3d后接种Tos3的防治效果达52.2％，比出发菌株Ata28的防治效果好。

Ata28 strain was mutated by UV with the most optimum mutant time, including 10, 20, 30s, that was chosen by the trial tests, considering the lethality rate and positive mutation rate of Ata28; it was found that 15 mutant strains were shown antagonism against Tbs3 strain by antagonism test on PDA plate, inhibition zone of mutant strains exceeded the original strain Ata28, and the maximum of inhibition zone reached 9.4mm; the mutant strain Uv-126 was found most effective in tobacco brown spot control and a rate of efficiency control reached 52.2% on tobacco plants three days after bacteria spraying.

采用硫酸二已酯诱变Ata28菌株，通过预备实验，计算Ata28菌株在不同诱变时间的致死率和正变率，选择该菌株的最佳诱变时间20min，40min，60min诱变；通过平板对峙培养，筛选出17个突变菌株在PDA平板上可明显抑制Tbs3的生长，抑菌带高于出发菌株Am28，抑菌带最大值达10.5 mm；经温室控病初筛和复筛试验，获得了对烟草赤星病有较好防效的突变菌株Des-66、Des-50，烟苗经Des-66、Des-50菌液处理3d后，接种Tbs3的防治效果达52.0％、56.6％，比出发菌株Ata28的防治效果好。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。