诱变的

In high selectivity enrichment culturing process and rapid screening procedure, the succinic acid producers with starchness and saccharide were isolated from bovine rumen.

本研究通过采用选择性较强的富集培养和快速准确筛选程序，从牛瘤胃中分离出适于淀粉质原料的琥珀酸产生菌，并进行相关的生理生化鉴定：通过紫外照射诱变育种得到琥珀酸高产突变株，优化培养基组成并确定最适发酵培养条件。

In order to prevent linear PCV2 cyclization, PCR mutagenesis was used to construct the first molecular clone (pSK-2PCV2) by ligating two copies of the complete PCV2 genome with the pBluescript SK vector. In addition, pSK-PCV2 and ds-PCV2 were constructed. PK-15 cells were transfected with above three infectious clones.

本研究应用PCR诱变技术解决了外源片段易于自连的难题，成功将2个PCV2 SD1株全基因组(DQ346683)头尾相接插入到真核生物表达载体pSK的多克隆位点中，构建重组质粒pSK-2PCV2；另外课题组成功构建含单个PCV2全基因组的pSK-PCV2和自身环化质粒ds-PCV2。

Enterobacter aerogenes was irradiated by He-Ne laser to optimize the laser mutagenesis condition, and to test the heredity, aciduric and hydrogen-producing potential of the mutant. An aciduric mutant with steady hydrogen-producing capability, which was able to grow at acidic pH of 3.0, was obtained. Its capability of hydrogen production was measured in the batch culture experiment.

闻建平，陈宇，刘铭辉(天津大学化工学院生物工程系，天津300072)摘要：运用He—Ne激光辐照产气肠杆菌，对激光诱变参数进行优化，考察了突变株的遗传稳定性、耐酸性和产氢特性结果筛选到一株遗传性状稳定的高产氢突变株，具有良好的耐酸性，在pH值3.0 时仍能生长。

There are many studies on Bombyx mandarina and Antheraea yamamai in or out of China, but no paper about them that were irradiated by He-Ne laser is found yet.

有关野桑蚕和天蚕的研究，国内外的报道较多，而激光辐射对野桑蚕和天蚕的诱变研究至今未见报道。

Several SP2 mutant plants were obtained from induced Impatiens balsamina L.seeds by space flight,and their blades were investigated in this paper.The writer calculated the K-S statistics and the levene statistics,also made use of SPSS 11.5 to have the independent-samples t test and the univariate in the prerequisite of some fundamental presumptions.

对航天诱变凤仙花SP2代若干单株的叶片长度和宽度进行了统计分析，对试验数据进行了柯尔莫哥罗夫-斯米尔诺夫检验，计算了样本的列文统计量，并采用Spss 11.5统计分析软件检验了基本假设的满足条件，进行了独立样本t检验。

One was the pqsA' positive expression plasmid constructed by cloning the pqsA'-lacZ fusion digested from pYHP441 into miniCTX-1, whose sequence was then integrated into wild type PAO-1 chromosome by biparental mating procedure. The other was pqsA-E operon knock-out plasmid whose sequence between pqsD and pqsE operon was inserted by tetracycline cassette by the site specific insertion mutagenesis strategy and the mutant constructed by biparental mating of S17-1 that harbored the plasmid and pqsA' positive expression mutant.

一种是酶切质粒pYHP441获得pqsA'-lacZ片段后，亚克隆入质粒miniCTX-1中，构建成PqsA'的阳性表达质粒，随后将构建的质粒，通过双亲交配过程整合入野生型铜绿假单胞菌株PAO-1染色体组中；另一种是通过点特异插入诱变策略，将四环素基因盒插入启动子pqsD和pqsE之间，构建的阴性质粒转化入大肠杆菌S17-1株后，和上述pqsA'阳性表达突变株进行双亲交配过程。

The thesis is researched from the three following aspects: the evaluation of quantitative method of flocculation, malt or barley on the effect of premature yeast flocculation, laser and chloridize lithium mutated and selected yeast with appropriate flocculation.

本课题主要从以下3方面进行研究：酵母絮凝性测量方法的评价、麦芽或大麦等原料对酵母絮凝性的影响、激光-氯化锂复合诱变筛选絮凝性适中的酵母。

A wild strain Rhodococcus rhodochrous was mutated by using UV light and Lithium chlorinate,and a mutant named tg1-A6 with high nitirlase activity was obtained.

采用紫外线和氯化锂诱变得到一株名为Rhodococcus rhodochroustg1-A6的菌种，该菌经过培养基和培养条件的优化能够产生高酶活的腈水解酶。

The feasibility of the protoplast mutagenesis for increasing productivity was studied on a Claviceps paspali strain, which was not easy to produce conidia under laboratory conditions.

为了提高在一般条件下不易产生分生孢子的雀稗麦角菌的产碱率，对原生质体诱变育种的条件和方法进行了探讨。

After treated by ultraviolet and EMS, a mutant M2-4-4 was produced from Cytophaga MCA-2, with a higher enzyme activity of 66U/ml.

经过多次富集和反复筛选，从海洋环境中筛选到69株具有卡拉胶降解活性的菌株，以产酶活力高、生物学性状优良的MCA-2菌株为出发菌株，进行复合诱变和自然选育，获得了一株酶活显著提高的突变株M2-4-4，发酵液产酶活力达到66U/ml。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。