诱变的

A human promyelocytic leukemia cell mutant (HL-60-AR) with deficiency in HGPRT and resistance to 8-AG was established by treat〓nt of the HL-60 cells with MNNG and prescreened in 0.3% agar RPMI-1640 semisolid medium containing 8-AG. The mutant cells show the following characteristics:(1) resistant to cytotoxic action of 8-AG and growing vigorously in medium containing 20ug/ml 8-AG;(2) capable of forming 〓〓 colonies in soft agar medium in the presenee of 8-AG:(3) highly sensitive to HAT selective medium and fail to growing in HAT medium of form colonies in soft agar medium containing HAT;(4) completely deficinet in HGPRT activity; and (5) no radioactive silver grains over the mutant cells due to the absence of -hypoxanthine incorporation showed by radioautography.

一、用MNNG诱变和8-AG进行抗性筛选，成功地建立了一株抗高浓度8-AG和完全缺失HGPRT活性的人早幼粒白血病细胞突变株(HL-60-AR)，该细胞能抵抗8-AG的毒性作用，对HAT选择培养基十分敏感，检测不到HGHRT活性，不能参入〓H一次黄嘌呤。

Breeding technology of industrial useful microorganisms was developed from conventional mutation and screening technology to mutagenesis breeding ,cross reeding,metabolic control breeding,gene engineering breeding and so on.

工业有益微生物的育种技术已从常规的突变和筛选技术发展到诱变育种、杂交育种、代谢控制育种和基因工程等。

EPS composition fermented with strain 7 and Streptococcus thermophilus together was stable and consists of glucose, galactose and rhamnose, but EPS with normal starters of Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus does not consist of rhamnose.

产粘7号菌在诱变前后与不产粘嗜热链球菌共同发酵产生的EPS单糖组成不变，都是由葡萄糖、半乳糖和鼠李糖组成；而不产粘嗜热链球菌与不产粘保加利亚乳杆菌产生的EPS不含鼠李糖，只有葡萄糖和半乳糖。

Methods The expression plasmid pSVvWF containing full-length cDNA of vWF was used to site direct mutagenesis by polymerase chain reaction and transitorily expressed in the COS-7 cells, vWF:Ag in the supernatant and the cell lysate between wild type and pSVAla737Glu vWF were measured.

用聚合酶链反应的方法对野生型的vWF全长表达质粒进行体外定点诱变，在COS-7细胞中进行表达。

Gas chromatography was employed to quantify the output of metabolization,and it was found that the yield of diacetyl for the mutant UV-3 was 18.7 fold to the wild-type 10293,up to 1.045g·L~(-1).Acetoin and ethanol yields were decreased by 48.4%and 71.4%,respectively.The yield of acetate was increased by 34.6%.

利用气相色谱测定代谢产物量，分析诱变前后菌株代谢途径中碳源的流向，结果表明突变株UV-3的丁二酮产量提高18.7倍，达到1.045g/L，乙偶姻产量降低48.4%，乙醇产量降低71.4%，乙酸产量提高34.6%，且遗传性质稳定。

Strain 9813 of Alcaligenes eutrophus derived by physical mutation can ferment fructose-glucose syrup to produce PHB.

经过物理诱变得到能利用果葡糖浆生产PHB的真养产碱杆菌9813，摇瓶发酵的适宜条件是：初糖浓度为2.5％～3.0％，硫酸铵浓度为0.3％，发酵时间48h，PHB浓度为14.7～14.8g·L〓。

In addition ,an asporogenous mutant G5was obtained from Bs C3-41 wild strain ,in which the formation of the spore was blocked at the stage III of the sporulation course .

此外，还诱变筛选到一株稳定遗传的Bs无芽孢突变株G5，其芽孢形成阻断于第三期，能够形成晶体，二元素表达量较高，可作为后选的工程菌受体。

Through three-step continual EMS mutation, the adenine autotrophy strain(Ml-3) and the adenine-requiring revertant strain(M2-8) were screened successively, and the resistant strain(M3-20) to ammonium ion variant was further screened.

将MY-1经连续3次EMS诱变，依次选育富含核黄素的腺嘌呤营养缺陷型菌株(M1-3)和腺嘌呤缺陷型的回复突变株(M2-8)。

The contents of Mn, Cu, Fe, Zn in the leave, flower, seed of Impatiens balsamina induced by space flight are determined by flame atomic absorption spectrometry. The measure parameters for FAAS are optimized.

采用火焰原子吸收分光光度法测定了航天诱变凤仙花的茎、叶、花、种子中Mn、Cu、Fe、Zn四种微量元素的含量，优化了原子吸收分光光度法测定参数。

In the study, physical and chemical mutagenesis and protoplast fusion technique were used to select strains with low-yielding diacetyl, acetaldehyde, total higher alcohols and other excellent characteristics.

本研究采用物理化学诱变和原生质体融合的方法，选育具有低产双乙酰、乙醛和总高级醇等优良特性的啤酒酿造酵母菌株。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。