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诱变处理

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Biological effects of autotetraploid rice after ion beam implantation of low energy N+ were studied.

以低能氮离子束为诱变源,对同源四倍体水稻进行注入处理后,对其生物学效应进行了研究。

Dioscorea parviflora was treated with colchicines at different concentration and time duration in vitro.

在离体培养条件下,比较了不同浓度,不同处理时间的秋水仙素对小花盾叶薯蓣丛生芽的诱变效果。

In the present experiment, the tender stems of Dracaena cambodiana were used as explants to establish a two-step tissue culture and regeneration system. The effects of types and concentrations of plant hormones on the induction of callus were studied. Based on these, the reactions of callus regeneration to different mutation-induced approaches (60Co irradiation, EMS and colchicines), the regeneration ability of the treated callus as well as the characters diversities (including the appearance traits and cytological observations) of the regeneration plants were investigated.

本试验以龙血树幼嫩茎段为材料,通过对龙血树幼嫩茎段两步培养法再生的研究,建立了龙血树幼嫩茎段离体再生体系,证明了愈伤组织的发生和激素种类与浓度的关系;并在此基础上研究了愈伤组织再生对不同人工诱变方法(60Co辐射、EMS、秋水仙素)的反应、被处理愈伤组织再生能力和再生植株性状多样性、以及再生植株不同生理角度的变异性,得到了2株变异株系。

Objective To screen higher-producing strains by the treatment of protoplasts of S. hygroscopicus FC904 with antibiotics and nitrous guanidine.

目的 选择抗生素和化学诱变剂处理雷帕霉素产生菌的原生质体,筛选高产菌株。

Crops of Brassica napus L., Linum usitatissmum L., Allium fistulosum L. and Lens culinaris Medic. were irradiated by 80 MeV/u 12C6+ ion beams with doses of 30, 90 and 180 Gy. The germination rates and heights of seedlings of M1 and M2 generation of these four plants were studied.

以能量为80 MeV/u的12C6+离子为诱变源辐照油菜、胡麻、大葱和兵豆的干种子后,研究了不同剂量处理对4种农作物M1和M2代种子出苗率及幼苗生长的影响。

Crops of Brassica napus L., Linum usitatissmum L., Allium fistulosum L. and Lens culinaris Medic. were irradiated by 80 MeV/u 12C(superscript 6+) ion beams with doses of 30, 90 and 180 Gy. The germination rates and heights of seedlings of M1 and M2 generation of these four plants were studied.

以能量为80MeV/u的12C(上标 6+)离子为诱变源辐照油菜、胡麻、大葱和兵豆的干种子后,研究了不同剂量处理对4种农作物M1和M2代种子出苗率及幼苗生长的影响。

The concentration of 6 mmol/L NaN3 treatment for 2 days was a suitable dosage for Phalaenopsis mutation induction in this test system.

根据以上结果认为,本体系合适的NaN3诱变剂量为6 mmol/L,处理2 d。

Using the mechanism of the nuclear reaction of accelerated 7Li +3 ions with the inner target in mutant material i.e 1H( 7Li, 7Be)n, the biological effects were studied. The wheat seeds were irradiated with the doses ranged from 1.416×10 10 ions/cm 2 to 1.416×10 12 ions/cm 2. It was found that the cell membrane ruptured, the plasmolysis occurred, the nucleus shape changed.

将加速器7Li+ 3 离子束与生物体内遗传物质发生1H( 7Li,7Be)n内靶特征核反应诱发核酸分子结构变化的机制应用于生物诱变,辐照剂量在 1 41 6× 1 0 10 粒子 cm2 - 1 41 6× 1 0 12 粒子 cm2范围内处理的小麦,观察幼苗叶片细胞亚显微结构,发现离子束注入引起了细胞膜的破裂,细胞发生质壁分离,细胞核形状变得不规则等。

The apical buds of Celosia cristata were used as the explants for the rapid propagation to compare the mutagenic effects treated with colchicines at different concentrations and time duration in tissue culture.

以鸡冠花顶芽为外植体,研究其离体快繁程序并在无菌体繁殖条件下探索了秋水仙素溶液不同浓度和时间处理对鸡冠花的诱变效应。

The concentrations and treatment time of EMS was related closely with the injury degree on the embryogenic callus.

在化学诱变剂 EMS对木薯胚性愈伤组织的处理中,EMS的浓度与处理时间对愈伤组织损伤程度有很大影响。

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