诱变处理

The results were as follows: The mutation rate could be increased effectively by the three treatments; the cooperation effect was better when TMV was treated by nitrous acid and heating.

采用高温、亚硝酸及两者的复合处理对烟草花叶病毒强毒株进行诱变，均可有效提高突变频率，而且复合处理还有较好的协同效应。

When treated with PYM ,mutation rates of ear length and tassel branch number were high, which were 4.81% and 4.77%. Mutagenic effect of PYM was worse than that of EMS and NaNa-Benzoyl.

PYM处理以穗长和雄穗分枝数的突变为高，4.81％、4.77％，其诱变效果没EMS和NaNs一苯甲酸胺处理好。3。

Two symptomless TMV mutants named TMV-O 17 and TMV- 152 respectively were obtained by treatment of heating, heating and nitrous acid combination.

通过高温处理、亚硝酸诱变及两者的复合处理对强毒株TMV-W进行诱变，筛选获得了两个弱毒株，记为TMV-017、TMV-152。

When EMS was applied, it was found that 2.0% EMS resulted the highest microkernel rate (15.6‰). Treating the materials with 2.0% EMS for 4 hours could lead to semi-lethal. Cytological observations found that chromosome aberration phenomena such as single microkernel, double microkernel, three microkernel, chromosome bridge, resort chromosome and syncytium were presented in the mutants.

EMS诱变途径中，浓度为2.0 %处理时间为4 h的组合获得最高的微核率（15.6‰），EMS浓度为2.0 %处理时间为4 h的组合使材料达到半致死；细胞学的观察看到了单微核、双微核、三微核、染色体桥、滞留染色体和合胞体等染色体畸变现象，再生苗中发现了1棵变异株。60Co辐射的诱变途径中，20Gy的剂量使不定芽半致死，再生苗中发现了1棵变异株。

Six bacteria and five mildews have been separated from 119 strains, wliich grew well on the culture media contained lignin. The result of taxonomic identification is that XI, X2 and X3 belong to Pseudomonas, X4 and X5 belong to Xanihomonas, X6 belongs to Mycoplana. A preliminary study has been carried out on the capacity of lignin degradation of the 6strains of bacteria, and strains X2 and X4 were chose to carry out the experiments. The most favorable temperature for the 2 strains is 30癈, and the pH is 7; After being treated by ultraviolet rays, activity of all the three kinds of lignin-degrading enzyme produced by the strains can be increased greatly, the bacteria increasing rate is remarkably improved.

从119个菌株中分离筛选出了6株细菌和5株霉菌，它们可以在含有木质素的培养基上良好生长：细菌分类学鉴定结果为X1、X2和X3：假单胞菌属，X4和X5：黄单胞杆菌属，X6：枝动杆菌属；对6株细菌降解木质素性能进行了初步的研究，选定X2和X4为实验用菌种，两株菌适宜的生长温度为30℃，最适初始pH值为7；经紫外线照射诱变法处理，可使它们产生的三种木素分解酶活性均有明显的提高，细菌增长速率比诱变前明显提高。

The results show that the suitable material for treatment is the bulb basal plate tissue with a thin part of scales after having been inoculated for 7 days,and regeneration dose is 50～60Gy,the optimum treatment dose for breading is 15～20Gy.In the small bulbs treated with 15Gy and 20 Gy,two mutants were selected,one is shorter with more leaves,the other expands faster.

结果表明，接种7D的外植体更适合作为水仙离体诱变的材料，其不分化剂量为50~60GY，半剂量为15~20GY，在10和15GY处理中，发现了两种小鳞茎突变类型，一种为叶片数增多的矮化突变体，一种为试管小球茎膨大速度加快，所以适宜辐射诱变的剂量为10~20GY。

The results show that die suitable material for treatment is the bulb basal plate tissue with a thin part of scales after having been inoculated for 7 days, and regeneration dose is 50~60Gy, the optimum treatment dose for heading is 15~20Gy. In the small bulls tinted with l5Gy and 20Gy, two mutants were selected, one is shorter with more leaves, the other expands faster.

结果表明，接种7d的外植体更适合作为水仙离体诱变的材料，其不分化剂量为50~60Gy，半剂量为15~20Gy，在10和15Gy处理中，发现了两种小鳞茎突变类型，一种为叶片数增多的矮化突变体，一种为试管小球茎膨大速度加快，所以适宜辐射诱变的剂量为10~20Gy。

The seeds of different varieties of mat rush were treated by 60Co γ-rays irradiation of 500, 1000, 1500 and 2000Gy. The seedlings cultured from treated seeds were used to detect the root activity, seedling growth, chlorophyll content, activities of polyphenol oxidase and catalase, and to investigate the effect of irradiation treatment on the physiological and biochemical properties of mat rush.

分别以500、1000、1500和2000Gy的60Coγ射线处理不同蔺草品种成熟干种子，并将其培养成实生苗，进行根系活力、叶绿素含量及多酚氧化酶和过氧化氢酶活性的检测，观察蔺草苗期的生长状况，从而研究不同辐照剂量对蔺草苗期生长及生理生化特性的影响，确定蔺草的适宜辐照诱变剂量，为下一步开展蔺草品种诱变改良奠定基础。

The effect of the two mutagenesis to the rate constant of the two microalage cell and the EPA and DHA cotents in the two miroalage cells proved that EMS was the better mutagen.

比较这两种诱变方法对海洋微藻的生长繁殖速度和脂肪酸含量的影响，结果证明采用化学诱变的处理方法更好。

Strain 7-12 was used as original strain through primary selection on plate. U\^ DES and NTG were choosed to continue to mutate them for 6 generations respectively.

通过平板初筛筛选出7-12作为出发菌株，以紫外线、硫酸二乙酯、亚硝基胍作为诱变剂，单一或复合诱变连续处理6代。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。