诱变处理

Pingyangmycin as、a mutagen was applied to crop mutational breeding in this experiment. The mutagenic effects of PYM on barley was studied by means of seed treatment.

本试验以平阳霉素为诱变剂处理大麦种子，研究了该诱变剂对大麦的诱变效应及其适宜的浓度。

Studies on the mutant breeding of high-yield gibberellins strain The protoplast of Gibberella fujikuroi m978 were treated by mutation breeding forhigh-yield gibberellins, LiCl mutation was better than DES mutation or UV irradiation ifsingle mutation, but any single mutation was worse than compound mutation by whichthe mutants were more overproducting and genetically stable.

采用传统诱变育种，筛选高产菌株在原生质体条件下，对赤霉素产生菌m978的原生质体进行菌种选育；使用单因子诱变方法，LiCl处理的效果优于DES诱变和UV诱变；复合诱变比单因子诱变更容易得到高产突变株，且获得的高产突变株稳定性更优。

In cider to breed new gern1asm for Narcissus tazetta, the bulb of Narcissus tazetta is used as explants. The adventitious buds is induced in MS media with hormone combination.γ-ray irradiation treatment of Narcissus tazetta combined with tissue culture is studied. Three materials, including the bulb basal plate tissues inoculated for 7 days, tissues transferred in fresh differentiation culture medium for three days after inoculated for 25 days, and small bulbs in tarn, are studied.

以带鳞片叶的鳞茎盘为外植体，接种在添加激素的MS培养基上，通过激素组合筛选合适的培养基获得不定芽，同时对在预分化培养基上接种7d的外植体、接种25d后转接到分化培养基上3d的外植体、诱导产生的试管小鳞茎进行辐照诱变处理，研究了60Coγ射线对中国水仙丛生芽分化以及小鳞茎生长的影响。

When treatment leaves in vitro with EMS only, two different ways were applied as bellow:① Treatment with mutagen solution.

在化学诱变剂EMS对离体叶片的处理中，EMS溶液浸泡处理的诱变效果好于EMS液体培养的诱变效果。

New variants of some plant viruses have been produced artificially in the laboratory by treating pure virus strains with mutagens.

用亚硝酸之类的诱变处理纯病毒株系，能在实验室内人为地使有些植物病毒产生新变种。

When treatment on the leaves in vitro of strawberry cultivars of All Star and Xinminxing by any mutagens, the result showed that the survival and regeneration rates have no difference .

对诱变剂的敏感性 '全明星'草莓和'新明星'草莓的离体叶片在各种诱变剂的处理下，成活率和再生率之间没有显著差异，表明两个品种对各种诱变剂的敏感性相似。

In this study, as a original strain, antagonistic bacterium Ata28 was mutated by UV, UV+LiCl, DES and Microwave irradiation; the very goal of the research is to improve the control efficiency of Ata28 strain against tobacco brown spot and obtain strains advanced both in antagonism and biological characteristics, further provides theoretics and practical experience to the production.

采用紫外线和氯化锂复合诱变Ata28菌株，通过预备实验，计算Ata28菌株在不同诱变时间的致死率和正变率，选择该菌株的最佳诱变时间10s，15s诱变；通过平板对峙培养，筛选出8个突变菌株在PDA平板上可明显抑制Tbs3的生长，抑菌带高于出发菌株Ata28，抑菌带最大值达9.7mm；经温室控病初筛和复筛试验，获得了对烟草赤星病有较好防效的突变菌株Uv1-190、Uv1-199，烟苗经Uv1-190、Uv1-199菌液处理3d后，接种Tbs3的防治效果达51.3％、54.9％，比出发菌株Ata28的防治效果好。

Ata28 strain exhibited marked suppression of Tbs3 strain which is the pathogen of tobacco brown spot on PDA plates, and the Ata28 strain had the good control efficiency of tobacco brown spot.

采用紫外线诱变Ata28菌株时，通过预备实验，计算Ata28菌株在不同诱变时间的致死率和正变率，选择该菌株的最佳诱变时间10s，20s，30s诱变；通过平板对峙培养，筛选出15个突变菌株在PDA平板上可明显抑制Tos3的生长，抑菌带高于出发菌株Ata28，抑菌带最大值达9.4 mm；在温室控病中，获得了对烟草赤星病有较好防效的突变菌株Uv-126，烟苗经Uv-126菌液处理3d后接种Tos3的防治效果达52.2％，比出发菌株Ata28的防治效果好。

Ata28 strain was mutated by UV with the most optimum mutant time, including 10, 20, 30s, that was chosen by the trial tests, considering the lethality rate and positive mutation rate of Ata28; it was found that 15 mutant strains were shown antagonism against Tbs3 strain by antagonism test on PDA plate, inhibition zone of mutant strains exceeded the original strain Ata28, and the maximum of inhibition zone reached 9.4mm; the mutant strain Uv-126 was found most effective in tobacco brown spot control and a rate of efficiency control reached 52.2% on tobacco plants three days after bacteria spraying.

采用硫酸二已酯诱变Ata28菌株，通过预备实验，计算Ata28菌株在不同诱变时间的致死率和正变率，选择该菌株的最佳诱变时间20min，40min，60min诱变；通过平板对峙培养，筛选出17个突变菌株在PDA平板上可明显抑制Tbs3的生长，抑菌带高于出发菌株Am28，抑菌带最大值达10.5 mm；经温室控病初筛和复筛试验，获得了对烟草赤星病有较好防效的突变菌株Des-66、Des-50，烟苗经Des-66、Des-50菌液处理3d后，接种Tbs3的防治效果达52.0％、56.6％，比出发菌株Ata28的防治效果好。

One L-isoleucine high-producing strain ISW330 with genetic marker was screened through shake tube primary screening, shake flask compound screening, genetic marker test and single clone isolation and continuous passage cultivation This strain could produce L-isoleucine 9.18g/L after fermentation under the fermentation condition without being optimized.

以黄色短杆菌HL41为出发菌株，通过硫酸二乙酯和紫外线诱变处理，经摇管初筛、摇瓶复筛、遗传标记验证、单菌落分离和连续传代，筛选出一株L-异亮氨酸生产菌株ISW330(Met~-+AEC~r+α-AB~r)。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。