诱变

In order to enhance the trehalose production, Y7 was used for mutation with EMS. Through primary screening and compound screening, strain L61 with high-yielding trehalose and deep neutral trehalase activity was screened.

实验对出发菌株Y7进行了EMS诱变处理，通过初筛、复筛，从中选育出一株能高产海藻糖同时具有较低海藻糖分解酶活性的菌株L61。

The medium composition and some fermentation parameters were studied by cross test and single factor experiments. The optimal conditions are as flows: Tryptone 0.76 %, Bean powder 1.0 %,initial pH 8.0, temperature 28 ℃, inoculation quantity 8 % and loading volume 30 %. Under optimal conditions, the maximum yield of biomass and microbial lipids reached 29.8 g/L and 11.7 g/L, respectively, which was 2.59 times and 2.05 times of those cultured in the original medium, after 9 days fed-batch co-fermentation in shake-flask.

采用单因子和正交实验对培养基组成和发酵条件进行了优化，结果表明，诱变菌在温度28 ℃，pH 8.0，接种量8 %，装液量30 %，蛋白胨0.76 %和豆粕1 %，分批补糖的最优发酵条件下发酵9 d，生物量和菌体油脂积累量分别达29.8 g/L和11.7 g/L，较出发菌提高了2.59倍和2.05倍，同时原料糖利用率达到99.4 %。

The repertoire includes amad (the dramatic entrance of the dancer on stage); thaat; tukra, tora, and paran; parhant, and tatkar.

演出曲目包括了市场准入数据库(戏剧性的舞者在舞台入口处)；诱变；tukra，托腊、与Paran；parhant、tatkar。

Using different dosages of He-Ne laser irradiated the developmental eggs of Antheraea yamamai. Compared with control plot, their hatchabilities were increased and their larval duration was influenced. Individual such as abnormal and premature mutants was also obtained. The blood protein and esterase isoenzyme of their pupa were analyzed with PAGE, found some changes in band r umber and activity. All those indicated that He-Ne laser has some inductive effects on physiological and biochemical characters of Antheraeayamamai.

不同剂量的He-Ne激光辐照天蚕发育卵，当代与对照相比较，孵化率提高、幼虫龄期经过缩短，产生附属肢畸变、早熟等变异体；对蛹血蛋白质、酯酶同工酶进行PAGE分析，其谱带数和活性也产生了变化，显示He-Ne激光对天蚕的生理生化性状具有诱变效应。

Soaking seed of rice and wheat with zymolytic liquid of Trichoderma harziaiarum, Trichoderma DWC and Trichoderma viride, comparing with germinate ratio, germinate force, root length, bud length and ratio of root to bud, the results show that 50 times dilute zymolytic liquid of Trichoderma harziaiarum has a relatively notable promote role for germinate of seed .The effect of Trichoderma viride to germinate of seed is not evidence. Trichoderma DWC for seed sprout has certain inhibition.

据此，采用哈茨木霉作为出发菌株，通过离子束诱变，从大菌落株中筛选出促进生长的高产突变株——h-13菌株，通过对用该菌株发酵液处理后的水稻及小麦的种子的各项生理生化指标的测定，发现该菌株比其它菌株更能促进水稻和小麦的活力和发芽率，三天时小麦的总根长为3.03cm，比对照的1.42cm的两倍还多，并且能够明显提高种子发芽后的淀粉酶和蛋白酶的活力，其活力都是对照的两倍左右。

Screening a Aspergilli with lipid production of 13.52% from soil, a mutant of superior Aspergilli was obtained with high lipid production of 18.75% through ultraviolet mutagenesis in order to empolder microbial oils resource.

为开发微生物油脂资源，从土壤中筛选出1株油脂含量为13.52%的曲霉，经紫外线诱变选育出1株油脂含量为18.75%的高产油脂曲霉。

The novel CMS line and its maintainer line with the xantha leaf marker, Huangyu A, were developed after a series of backcrosses, and were appraised by the Department of Science and Technology, Zhejiang Province.

用300Gyγ射线辐照Ⅱ-32B干种子，在诱变后代筛选到全生育期黄叶突变体WYB，经多代连续回交转育，育成黄叶标记不育系黄玉A，并通过了浙江省科技成果鉴定。

A it is predominantly derived from the initial variety or from a variety which itself was predominantly derived from the initial variety, while retaining the expression of essential characteristics that result from the genotype or combination of genotypes of the initial variety; an essentially derived variety may have been obtained by the selection of a natural or induced mutant or of a somaclonal variant, by the selection of a variant individual from plants of the initial variety, by backcrossing, by a transformation by genetic engineering, or by any other similar method

一个品种主要由原始品种演化而来，或主要由原始品种的实质性派生品种演化而来，同时保留了来自原始品种基因型或基因型组合的基本特征的表达；一个实质性派生品种可能通过选择自然变异或诱变或体细胞无性变异、原始品种植株的个体变异的选择、回交、基因工程的改造或任何其它类似方法而获得。

The mutagen mechanism is presumed single factor dominant.(4)There was 85 percent transvertion in mutagen spectra, and they were all G:C→T:A transvertion. The maximal possibility of mutagen mechanism is analyzed as followed:ion beam implant → breakage cell surface and enter the interior → stimulate plentiful radical born → radical oxidize and damnify DNA → G becomes 8—oxoG → 8—oxoG pairing A → result in G:C→T:A transvertion(5)Production of transition was presumed that ion beam implantion damnified DNA, stimulate activity of MMR interveniented by methyl reducing.

诱发突变谱中碱基颠换比例是0.85，且是全部G：C→T：A颠换，推测低能离子束诱变鼠伤寒沙门氏菌得分子机制可能为：离子注入→通过破坏细胞表面进入内部→刺激产生大量自由基→自由基氧化损伤DNA→G变成8氧—7，8—二氢鸟嘌呤(8—oxoG)→8—oxoG与A配对→导致G：C→T：A颠换。

The mutation and the screening of the original fungus were carried out by the ultraviolet radiation method to obtain a mutant species UV-A that can depredated the crude protein in the rape cake to 15.46%,which is 1.4 times that of the original fungus.

采用紫外照射法对原始菌株进行诱变和筛选，得到一株降解饼粕中粗蛋白能力较强的变异菌株UV -A ，能使饼粕中粗蛋白的含量降到15 。4 6 %，是原始菌株的1.4倍。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。