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诱变

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The aqueous extract of Pulsatilla Chinensis Regel showed antimutagenesis and antioxidation activities.

白头翁水提物具有抗诱变和抗氧化作用。

The optimal concentration of PYM applied was 30 μg/ml.

平阳霉素对大麦诱变的适宜浓度为30μg/ml左右。

The results showed that PYM could dramatically inhibit callus induction and differentiation of explants.

L-1平阳霉素对小菊品种意大利红离体培养的叶片和茎段进行诱变处理。

When treated with PYM ,mutation rates of ear length and tassel branch number were high, which were 4.81% and 4.77%. Mutagenic effect of PYM was worse than that of EMS and NaNa-Benzoyl.

PYM处理以穗长和雄穗分枝数的突变为高,4.81%、4.77%,其诱变效果没EMS和NaNs一苯甲酸胺处理好。3。

A human promyelocytic leukemia cell mutant (HL-60-AR) with deficiency in HGPRT and resistance to 8-AG was established by treat〓nt of the HL-60 cells with MNNG and prescreened in 0.3% agar RPMI-1640 semisolid medium containing 8-AG. The mutant cells show the following characteristics:(1) resistant to cytotoxic action of 8-AG and growing vigorously in medium containing 20ug/ml 8-AG;(2) capable of forming 〓〓 colonies in soft agar medium in the presenee of 8-AG:(3) highly sensitive to HAT selective medium and fail to growing in HAT medium of form colonies in soft agar medium containing HAT;(4) completely deficinet in HGPRT activity; and (5) no radioactive silver grains over the mutant cells due to the absence of -hypoxanthine incorporation showed by radioautography.

一、用MNNG诱变和8-AG进行抗性筛选,成功地建立了一株抗高浓度8-AG和完全缺失HGPRT活性的人早幼粒白血病细胞突变株(HL-60-AR),该细胞能抵抗8-AG的毒性作用,对HAT选择培养基十分敏感,检测不到HGHRT活性,不能参入〓H一次黄嘌呤。

Breeding technology of industrial useful microorganisms was developed from conventional mutation and screening technology to mutagenesis breeding ,cross reeding,metabolic control breeding,gene engineering breeding and so on.

工业有益微生物的育种技术已从常规的突变和筛选技术发展到诱变育种、杂交育种、代谢控制育种和基因工程等。

EPS composition fermented with strain 7 and Streptococcus thermophilus together was stable and consists of glucose, galactose and rhamnose, but EPS with normal starters of Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus does not consist of rhamnose.

产粘7号菌在诱变前后与不产粘嗜热链球菌共同发酵产生的EPS单糖组成不变,都是由葡萄糖、半乳糖和鼠李糖组成;而不产粘嗜热链球菌与不产粘保加利亚乳杆菌产生的EPS不含鼠李糖,只有葡萄糖和半乳糖。

The dissertation focuses on the breeding of Rhizopus chinensis 12# producing a fibrinolytic enzyme, optimization of solid-...

本论文对产纤溶酶的菌株根霉12#进行了诱变育种,优化了根霉12#产纤溶酶的固态发酵和液态发酵条件,建立了根霉纤溶酶制备分离纯化的方法并进行了优化,研究了根霉纤溶酶的性质。

The advances and existing problems concerning in genetics especially on the sex-inheritance and tolerance to papaya ring spot virus, the genetic improvement (cross breeding, mutation breeding, introduction breeding, culture in vitro, molecular marker assistant selection and bioengineering) on papaya are reviewed; especially on the sex-inheritance, distant hybridization and embryo culture, and breeding for disease resistance to PRSV with gene engineering are discussed in detail.

就番木瓜的株性和抗病性等性状遗传、杂交育种、人工诱变育种、离体培养、分子标记及基因工程育种等现代生物新技术育种方面的研究进展及相关问题进行了评述,重点综述了番木瓜株性遗传、远缘杂交与胚胎学、基因工程在抗环斑型花叶病毒病品种培育及分子标记辅助选择育种应用的研究进展,并分析了今后番木瓜育种的发展趋势。

Compared with hybrid transferring breeding, the radiation induced-mutation had advantages of simple method and short cycle.

辐射诱变与杂交转育方法相比,具有方法简单、周期短的优点。

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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