该受的

Who love my downfall and it's just too cold you show me true friend baby i was so so you might as well call me up physically you know, you got me only into extremes and i can't believe it and don't know what to think sometime around it was so love-sick now it's so sick cause they ain't doubt love your hint is apart and the day between us wasn't enough and i know i feel good time's come i thought they stay, things are done and become word-made angels came but they left two day had you slip away listen now burning empty still this can't be but even now we're not that happy hotel motel it's hot in hell free from myself but now left with no home i want you to know i never would have all figure out that when you came now it could have been forever now it does bring me down the high now the low up and down we go put myself too close got burnt night tones it feels like i'm sinking in the dead sea don't we care the space inside us so empty it's like it's over before be gone this song is over now so was i want

谁爱我的灭亡它的公正太冷你给我看真正的朋友宝宝我是这么这么你还不如叫我了身体你知道，你明白我只有到极端我不能相信这更不知道该怎么想围绕某个时候，它是如此爱生病现在，它的病事业，他们是不会怀疑的爱你的暗示是，除了及国庆，我们之间是不足够而我知道我感觉很好，当时的来我还以为他们留下来，这样做，并成为词国产天使来到，但他们离开二天你已经失去了听燃烧空仍不能但即使是现在，我们没那么开心酒店汽车旅馆，它的热地狱，不受自己但现在只剩下没有家我要你知道我从未想到自己会所有图说，当你来到现在它可能会被永远现在它并带我下来高，现在低向上与向下走把自己太接近弄得焦头烂额的夜晚铃声感觉就像我沉没在死海我们不小心的空间在我们的内心世界，使空它就像它的超过前一去不返这首歌是现在这样做主要是我想

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

We used Aeromonas hydrophila which we separated from the diseased Paralichthys olivaceus to made a inactivated whole cell bacterin .We injected this vaccine in the mice and the Paralichthys olivaceus .Both the mice and the Paralichthys olivaceus gained a good protection of 80% and 70%.The fish with dipping immersion gained a good protection of 85%,and the incidence of disease was 6%.We detected the antibody level in the antiserum by agglutination reaction and indirect ELISA which seemed to be immunogenic crucially.We anatomized the mice then we found that all the purtenance of the immunzied mice are healty.All of this proved that the vaccine is effective.

本实验将所分离到的嗜水气单胞菌用终浓度为0.2%~0.5%的甲醛对菌体进行灭活，制备了灭活疫苗，并优化灭活疫苗的灭活条件；将制备的灭活疫苗分别注射小鼠和牙鲆，均取得了较为理想的效果，其中小鼠的免疫保护率达到80%，牙鲆的免疫保护率达到70%；采用渗透压差法大规模浸泡免疫牙鲆也取得了很好的效果，浸泡免疫过的牙鲆发病率仅为6%，攻毒后免疫保护率为85%；通过应用间接ELISA方法检测受免动物的抗血清，并将牙鲆的抗血清注射新西兰白兔，从而获得兔抗鱼血清，更便于检测，结果都表明动物体内产生了较高的抗体水平；剖检攻毒后的动物，发现免疫过的动物内脏健康无异样，表明该疫苗安全有效，从而为防治牙鲆腹水症开辟了一条新途径，也为进一步制备其基因工程疫苗奠定了基础。

Main research contents as follows:(1) The four destruction models and respective stability analysis methods are discribed in this paper, whose working mechnism and calculating methods are given. In addition, some defects of every method are also discussed ;(2) On the basis of traditional active soil pressure method and expirical siol pressure method , the calculating model of the laternal earth pressure which is a tetragon with the largest value in the center side is addressed.

主要工作如下：(1)本文给出了复合土钉墙的稳定性分析方法，该方法介绍了复合土钉墙的四种破坏类型，分析了每种破坏类型的受力机理，并给出了相应的计算方法和计算方法中的一些不足；(2)在传统的主动土压力和经验土压力的基础上，建立了土钉墙中间大、上下小的四边形状分布的侧向土压力的计算模式。

In addition, some defects of every method are also discussed ;(2) On the basis of traditional active soil pressure method and expirical siol pressure method , the calculating model of the laternal earth pressure which is a tetragon with the largest value in the center side is addressed.

主要工作如下：（1）本文给出了复合土钉墙的稳定性分析方法，该方法介绍了复合土钉墙的四种破坏类型，分析了每种破坏类型的受力机理，并给出了相应的计算方法和计算方法中的一些不足；（2）在传统的主动土压力和经验土压力的基础上，建立了土钉墙中间大、上下小的四边形状分布的侧向土压力的计算模式。

Moreover, the accuracy analysis for different measurements and parameters(such as time measure error, azimuth measure error, buoys error, baseline length and sound velocity fluctuation) are given respectively. To make the best use of the observation datas, the weighted least square localization algorithm is used to improve the accuracy in bistatic sonar. The simulation results show that the positioning accuracy of this algorithm is higher than the other four algorithms, with which the positioning accuracy in stations areas and side areas can be improved effectively.

同时，通过对系统各个因素，如系统的各观测量的测量误差、站址误差、基线长度、声速波动等参数对算法性能的影响，得出了各方法定位误差的分布规律；为了充分利用双基地声纳系统的观测数据，提高系统的定位精度，采用加权最小二乘方法对系统的数据进行优化处理，仿真结果表明该方法定位精度明显高于基本方法的定位精度，能有效的改善发射站和接受站侧边区的定位精度，且定位性能受站址测量误差的影响较小。

Lightened on the turbine valve controller designed with feedback linearization theory and introduced nonlinear tracking-differentiator, a novel nonlinear PID valve controller was proposed. This controller can fit not only to plant with some model error and random disturbance in variable, but also to the variety of the structure and operating conditions of power system. The transient and dynamic characters of the controller are both satisfactory when big and small disturbances added. The good performance, wide stable margin and heavy robustness of the valve controller proved that it is a great practical application to enhance the stability of power system.

结合反馈线性化法汽轮发电机组的汽门控制研究成果，引入非线性跟踪微分器和非线性度变换控制，进行了发电机组的非线性PID汽门控制器设计，该控制器不仅能适应一定的系统模型误差与变量的不确定性干扰，而且对于电网结构和运行工况的变化有着较强的适应能力，在受大、小扰动的情况下，呈现了较好的暂态特性和动态品质，具有较好的控制品质、较高的稳定裕度和较强的鲁棒性能，有利于进一步改善电力系统的稳定性，显示了其较强的实用性。

We have the honour to make this statement on human rights, sexual orientation and gender identity on behalf of 1 - We reaffirm the principle of universality of human rights, as enshrined in the Universal Declaration of Human Rights whose 60th anniversary is celebrated this year, Article 1 of which proclaims that "all human beings are born free and equal in dignity and rights"; 2 - We reaffirm that everyone is entitled to the enjoyment of human rights without distinction of any kind, such as race, colour, sex, language, religion, political or other opinion, national or social origin, property, birth or other status, as set out in Article 2 of the Universal Declaration of Human Rights and Article 2 of the International Covenants on Civil and Political, Economic, Social and Cultural Rights, as well as in article 26 of the International Covenant on Civil and Political Rights; 3 - We reaffirm the principle of non-discrimination which requires that human rights apply equally to every human being regardless of sexual orientation or gender identity; 4 - We are deeply concerned by violations of human rights and fundamental freedoms based on sexual orientation or gender identity; 5 - We are also disturbed that violence, harassment, discrimination, exclusion, stigmatisation and prejudice are directed against persons in all countries in the world because of sexual orientation or gender identity, and that these practices undermine the integrity and dignity of those subjected to these abuses; 6 - We condemn the human rights violations based on sexual orientation or gender identity wherever they occur, in particular the use of the death penalty on this ground, extrajudicial, summary or arbitrary executions, the practice of torture and other cruel, inhuman and degrading treatment or punishment, arbitrary arrest or detention and deprivation of economic, social and cultural rights, including the right to health; 7 - We recall the statement in 2006 before the Human Rights Council by fifty four countries requesting the President of the Council to provide an opportunity, at an appropriate future session of the Council, for discussing these violations; 8 - We commend the attention paid to these issues by special procedures of the Human Rights Council and treaty bodies and encourage them to continue to integrate consideration of human rights violations based on sexual orientation or gender identity within their relevant mandates; 9 - We welcome the adoption of Resolution AG/RES.

附录：关于性倾向和性别认同联合声明中英文联合声明中文我们很荣幸地代表做出这一关于人权、性倾向和性别认同的声明 1 -如同宣布&所有人类生而自由，平等享有尊严和权利&的《世界人权宣言》——今年是该宣言发表60周年——第1条中所昭示的那样，我们重申人权的普遍性原则； 2 -如同《世界人权宣言》第2条和《公民、政治、经济、社会和文化权利国际公约》第2条及《公民权利和政治权利国际公约》第26条中所陈述的那样，我们重申所有人都有权享有人权，不受任何种类——如种族、肤色、性别、语言、宗教、政治观点或其他观点、国家或社会出身、财产、血统或其他身份——的区别对待； 3 -我们重申要求将所有人权平等应用于所有人类，无论其性倾向或性别认同如何的非歧视原则； 4 -我们深切关注基于性倾向和性别认同的侵犯人权和基本自由的现象； 5 -我们还对世界各国由于性倾向或性别认同而针对人们的暴力、骚扰、歧视、排斥、污名化和偏见，以及这些做法破坏了遭受这些虐待者的完整性和尊严感到不安； 6 -我们谴责基于性倾向和性别认同的侵犯人权行为，无论这些行为发生在哪里，尤其是以此为由使用死刑，法外处决、即审即决或任意处决，酷刑和其他残忍、不人道或有辱人格的待遇或处罚的做法，任意逮捕或拘留，以及剥夺经济、社会和文化权利，包括健康权； 7 -我们回想起2006年，五十四个国家在人权理事会面前发言，请求理事会主席提供一个未来在适当的理事会会议上讨论这些侵犯人权行为的机会； 8 -我们赞赏人权理事会特别程序和条约机构对这些问题给予的关注，并且鼓励他们继续将对基于性倾向和性别认同的侵犯人权事件的考虑纳入其有关权限中； 9 -我们欢迎美洲国家组织大会在其2008年6月3日的第38次会议上通过了关于&人权、性倾向与性别认同&的AG/RES。

Moreover, since subjection to the Roman pontiff is necessary for salvation for all Christ's faithful, as we are taught by the testimony of both sacred scripture and the holy fathers, and as is declared by the constitution of pope Boniface VIII of happy memory, also our predecessor, which begins Unam sanctam, we therefore, with the approval of the present sacred council, for the salvation of the souls of the same faithful, for the supreme authority of the Roman pontiff and of this holy see, and for the unity and power of the church, his spouse, renew and give our approval to that constitution, but without prejudice to the declaration of pope Clement V of holy memory, which begins Meruit .

此外，由於受罗马教皇是必要的救赎所有基督信徒，因为我们所教的证词都圣经和罗马的父亲，并宣布宪法所教宗波尼法爵八世的快乐记忆，也是我们的前任开始的墨西哥国立自治大学圣，因此，我们批准，本神圣理事会，拯救灵魂的同一忠实，最高权威的罗马教皇和罗马教廷的，并为团结和力量教会，其配偶，更新，使我们的批准，该宪法，但不妨碍宣布教皇克莱门特第五神圣记忆，开始金额。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了