试验物

E. methyl jasmonate +methyl salicylate, MeJA +cis-hexenal, MeJA +trans-hexenal, MeJA +benzothiazole, and then the induced activities of three defensive enzymes including peroxidase, polyphenol oxidase and phenylalanine ammonia lyase were measured.

以1年生合作杨扦插苗为试验材料，利用茉莉酸甲酯分别与水杨酸甲酯、顺式己烯醛(cis-hexenal)、反式己烯醛(trans-hexenal)和苯骈噻唑混合的4种挥发物熏蒸合作杨叶片，检测过氧化物酶、多酚氧化酶及苯丙氨酸解氨酶3种防御酶的活性变化。

The preparation and operation of automatic sampling instrument, the selections of light reported andmeasurement way are described in betail in the paper.

文中详细叙述了自动进液装置的制作，使用方法，光源及测量方式的选择，试验确定了Au－溴－结晶紫离子缔合物萃取显色体系的最佳分析条件。

In the RAPD system of Bryaceae the optimal concentration of template DNA was 2ng/μl, primer 0.4μmol/L, dNTP 0.2mmol/L, Mg2+ 2mmol/L, Taq DNA polymerase 1.25U, total volum 25μl. Several DNA samples were selected to choose the compatible primers.

通过单因素和正交试验对影响RAPD的因素进行考查，建立了优化的RAPD反应体系：dNTP为0.2mmol/L，随机引物0.4μmol/L，Taq DNA聚合酶1.25U，Mg2+为2mmol/L，模板DNA为50ng，扩增体系为25μl。

Six piglets of each group were selected on 42-d-old to measure the caecal and colonic microflora population and PH. The result showed that caecal and colon E.

试验开始第7天，每组各随机选6头仔猪，麻醉后剖腹取肠道内容物以测定盲肠和结肠大肠杆菌、乳酸杆菌及双歧杆菌浓度和pH。

The crystalline behavior and mechanical properties of the blends were investigated by differential scanning calorimetry and tensile measurements.

用差示扫描量热仪和电子拉力试验机分别测定共混物的热性能和力学性能。

The purpose of this study was analysis and tracking the heredity stability of Alpaca through amplifying special fragment from mitochondrion genome. A pair of primer was designed according to those sequences of Camelidae animal submitted in GaneBank and a fragment of 1014 bp was cloned.

摘 要：本试验对GaneBank中登录的部分骆驼科动物线粒体12srRNA/tRNA-Val/16rRNA基因序列进行同源性比较，并借助DNAstar软件设计引物，扩增并进行序列分析，旨在揭示其遗传变异的规律。

In order to direct the construction of plant germplasms by elucidating the relatives among plants at the level of gene, CYP86MF gene analogues from 11 species of 6 genera in Cuciferae were respectively obtained by PCR strategy using gene specific primers designed from conserved regions of CYP86MF gene reported. Sequence comparisonindicated that the similarities among the genes at nucleotide level were over 80%, and the similarities at amino acid level remained above 70%. The differences between the genes at nucleotide and amino acid level between species were 1.0%~ 5.7% and 2.6%~ 7.3% respectively, while those between genera 5.6%~ 22.5% and 7.3%~ 31.2%, respectively. Phylogenetic analysis showed that Brassica was closely related to Raphanus, followed by Rorippa Scop, Arabidopsis Heynh, Capsella Medic orderly, most distantly related to Orychophrogmus.

为了从分子水平阐明十字花科植物间的亲缘进化关系，给植物种质资源的创建提供理论依据，试验根据课题组已报道的CYP86MF基因编码的氨基酸保守区域设计特异引物，运用PCR技术分别从十字花科6个属11个物种中分离克隆到了CYP86MF基因的同源序列，经比较分析，结果表明：这些同源序列的相似性达80%以上，所推导的氨基酸序列相似性达70%以上，且两者种间差异分别为1.0%~ 5.7%和2.6%~ 7.3%，属间差异分别是5.6%~ 22.5%和7.3%~ 31.2%；由氨基酸序列构建的分子系统树可知，在亲缘进化关系上芸薹属与萝卜属较近，其他依次为蔊菜属、拟南芥属、荠菜属，而与诸葛菜属最远。

Carbonitride precipitation behavior of Nb microalloyed steel during holding at high temperature and low temperature was studied through thermal simulation.

通过热模拟试验，研究了Nb微合金钢中的碳氮化析出物在高温及低温等温过程中的析出行为。

The achievement of this project can be briefly summed up as following:(1)the carbon contributing to karst processes mainly comes from ecological system itself under the condition of existence of vegetation and soil covers;(2)results indicates that the depositing processes of rainfall to CO2 is an important "sink" to global carbon cycle;(3)owing to the differences of vegetation and surface covering, the adjusting effect of ecosystem to environmental humidity, temperature and CO2 concentration near surface air which indicates different benefit of preventing water and soil from lose is marked;(4)the preponderant species of vegetation in the karst ecosystems which have different kinds of carbonate rocks are obviously variant, and this situation results to the clearly differences of the micro － habitat , structure of surface cover and water holding capacity;(5) the relevant study showed that the carpophagous animals and birds make a important role in the spreading of vegetation seeds. The designed experience for the study of seed reservoir was not able to obtain a satisfactory result.

主要研究成果包括：通过对表层系统碳库的研究，认为在有土壤和植被覆盖时，岩溶作用过程中的碳主要来自生态系统本身而不是母岩；降雨对大气碳具有重要&沉淀&作用，是碳的一个重要汇；不同植被类型表层岩溶系统对系统水份、空气湿度、气温、土温和近地表 CO2浓度等具有明显调节作用，具有不同的水土保持效益；不同母岩的岩溶生态系统的植被优势种、属差异明显，由此形成的小生境、地被物及其持水性差异巨大；对典型岩溶表层系统种子库的试验研究没有取得满意结果，但食果动物、鸟类对种子传播起到一定作用。

The repeatability and reproducibility of microwave testing chemical composition of ramie were studied. Five microwave ovens were applied in this experiment to test ceride, hydrotrope, pectin and hemicellulose in raw ramie samples .

摘要研究了苎麻化学成分微波检测法的重复性和再现性，试验采用苎麻原麻样品在5台不同的微波炉上提取测定脂蜡、水溶物、果胶及半纤维素。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。