试验物

By using Roche kit as standard, the amplification efficiency of different reagents were compared by Roche Light Cycler instrument for establishing real-time PCR reaction system. The primers were designed based on signature sequence of chromosomal DNA fragment and caf 1 gene in plasmid pMT1 of Y.pestis. And sensitivity and specificity assay were done with serial diluted Y. pestis EV76 strain and other bacterias. Then 275 Y.

采用SYBRGreenI随机掺入法，以Roche试剂盒为标准，比较两公司的DNA聚合酶，用鼠疫菌310 0 4建立荧光PCR反应体系；针对鼠疫耶尔森氏菌特异的染色体标识序列和质粒上F1抗原基因设计引物，检测其灵敏度和特异性，以盲测试验进行验证；在此基础上鉴定2 75株鼠疫耶尔森氏菌，并测试该法检测脏器污染模拟标本的灵敏度。

According to the composition of elements of fuel,thermochemical forms,combustion heat,and time-dependent relations of oxygen concentration acquired during fullscale tunnel fire experiment,the formulae are given for calculating di- mensionless burning rate, released heat rate ,and ratio of medium decalescence.

根据燃料的元素构成、热化学反应式、反应物和生成物的焓变以及燃烧过程中监测到的烟气中氧气浓度随时间变化规律，提出了计算某一时刻燃料燃烧速率、释热速率和介质吸热率的关系式；通过实际规模的火灾试验，对其数据的计算表明，对木质类巷道火灾，最大比燃烧速率为0.193，最大释热速率约为1～3.5 MW，介质的吸热率为22

Free fatty acid separation from vegetable oil deodorizer distillate using molecular distillation process.

尿素包合和超临界CO2 萃取相结合分离豆油脱臭馏出物中α- VE 的试验研究。

Study on bond and diamond of diamond core drill bit for reinforced concrete;2. Also glass ceramic is used as the bond of CBN grinding wheel by examining the properties of new bond .

研究了微晶玻璃中加入低熔物、成核剂后对微晶玻璃热处理曲线、微晶量及微晶体尺寸的影响，并通过测试改性微晶玻璃的软化温度和热膨胀系数对CBN磨粒的把持强度，进而将微晶玻璃用作CBN砂轮结合剂的作用作了研究，经过在数控凸轮轴磨床上的试验证明，微晶玻璃结合剂的CBN砂轮的磨削效果很好。

Now the research on HPL focus on dicotyledon, and the research on HPL of monocotyledon still havent been reported. In this paper, the research on cloning of rice HPL and enzyme activity assay were carried out and several result were obtained.1. Enzyme activity assay of rice HPLBased on the method of measure HPL Enzyme activity in other plants, the means on preparation of hydroperoxides and measurement rice HPL activity were set up. The 9-HPL arid 13-HPL activity of several rice varieties with different storability and different dormancy were tested.

本试验建立了水稻的HPL酶活性测定体系，测定了水稻萌发过程中的HPL酶活性，并进行了水稻HPL基因的克隆及表达研究，结果如下： 1、水稻HPL的酶活性测定在参考其它植物HPL酶活性测定方法的基础上，建立了水稻的HPL底物发生体系和酶活性测定体系，并测定了不同贮藏性水稻品种萌发过程中的种胚9-HPL、13-HPL活性和不同休眠性水稻品种种子的9-HPL、13-HPL酶活性。

This paper described such a comprehensive model based on a great number of documents, which takes into consideration details including air entrainment of fuel liquid zones, unsteady heat transfer during droplet evaporation, difference between premixed and entrain dominant combustion rate, compression factor of actual gas in cylinder pressure calculation and minimization of free energy during chemical equilibrium.

摘 要：对增压直喷柴油机的缸内空气卷吸、瞬态燃油液滴蒸发、燃烧过程的分段特征、化学反应平衡的最小自由能机理、实际气体性质修正、燃烧排放物生成等进行了分析，结合缸外的喷油过程仿真及进排气过程的容积法，建立了一个综合的研究性能及排放的准维多区模型，并进行了两个机型多个工况的实机试验比较。

Object: The antimicrobial effects of the water, 60% alcohol, 90% alcohol extract and petroleum ether, ethyl acetate, n-butanol components from Pomegranate rind against 3 kind of pathogenesis bacterial and 14 kind of epiphyte were accomplished.

目的：对石榴皮的水、60%乙醇、90%乙醇提取物以及石油醚、乙酸乙酯、正丁醇萃取物对3种细菌和14种真菌的体外抗菌试验进行了研究。

Sample preparation, chromatogram conditions, extractant and section of the standard sample are tested and discussed.

对样品制备、色谱条件、萃取剂和内标物选择进行了试验和讨论。

But it could not be concluded that root exudates detected in the experiment play key role in Zn hyperaccumulating.

这说明本试验条件下测定的根系分泌物组分在东南景天超积累Zn过程中并不起主导作用。

And the last, the effects of fermented cottonseed-hull on dairy performance were studied with feed tests. In experiment 1, Cottonseed-hull and comminuted cottonseed-hull were fermented with complexing strains of microbials including EM and probiotics, using seven pairs of formulation have been formed by corn meal, molasses and fecula combined with urea and zein meal.

试验一利用EM菌液与益生素混合扩大培养后的复合菌液，选用玉米粉、糖蜜、淀粉三种炭源和尿素、玉米蛋白粉两种氮源共组成了7组发酵底物配方，将棉籽壳分成微粉样与原始样，发酵结束后对测定结果进行统计分析。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。