试验方法

To evaluate the safety of the DNA vaccine pVAX1/F1-V against plague, the purity of plasmid DNA pVAX1/F1-V was detected by SDS-PAGE and gelose electrophoresis method; pasmid DNA was detected by PCR in tissues of BALB/c mice immunized with the plasmids intramuscular injection.

为了研究鼠疫DNA疫苗pVAX1/F1-V质粒的安全性，试验运用SDS-PAGE电泳和琼脂糖电泳等方法检测了鼠疫DNA疫苗pVAX1/F1-V质粒的纯度，并以小鼠为动物模型进行了质粒pVAX1/F1-V的急性毒性和长期毒性试验，用PCR方法检测外源基因在小鼠组织中的分布情况，用ELISA、ELISPOT方法检测了其自身的免疫反应。

From these researches as above, this paper found a new method to analysis salty soil road engineering properties by phase equilibrium theory, which provides a new angle of analysis on salty soil properties, experiment and engineering practice in these ways as follows:(1) Analysis the disadvantage of traditional salty soil experiment;(2) Analysis the disadvantage of current salty soil definition by phase equilibrium theory, and gives the chart of distinguish salty soil by the condition of hydrography and climate change;(3) Analysis the mechanics of subgrade frost boiling based on the change rule of liquid content;(4) Based on the change rule of ingredients content and volume in salty soil, analysis the mechanics of subgrade salt heaving and the restraining method by adding sodium chloride.

通过以上研究，本文初步建立了以相平衡理论为核心的盐渍土路用工程性质分析方法，该方法对盐渍土工程性质的理论分析、试验研究以及工程实践提供了新的研究思路与方法，具体体现在：(1)分析了传统的盐渍土试验存在的缺陷；(2)分析了现行盐渍土定义的不足，并以相平衡理论为基础，结合水文、气候条件的变化给出了判别盐渍土的诺谟图；(3)分析了盐渍土盐溶液含量的变化规律，并从盐渍土含液量变化规律出发，分析了盐渍土路基较非盐渍土路基翻浆严重的机理；(4)分析了盐渍土各固相含量及其体积的相变规律，并在此基础上分析盐渍土路基的盐胀机理，以及采用添加氯化钠来抑止硫酸盐盐渍土的盐胀机理

This paper was put forward to the method, principle and procedure of third infilling in order to the economic effective development based on fine reservoir description and remaining oil research.

本文以大庆油田三次加密矿场实践为依据，通过对试验效果的系统评价研究，提出了对三次加密井工艺技术的要求；分析了低效井的原因，研究了降低低效井比例的有效方法，并在杏一～三区矿场试验中得到了验证；通过对加密井单井经济技术界限研究，搞清了三次加密调整的投资成本构成，制定了三次加密调整井增加可采储量、厚度和产量界限；指出了三次加密潜力对象及其分布特征；提出了以精细地质研究为基础搞清薄差储层沉积特征及空间展布、以剩余油综合描述为手段定量描述各沉积单元的剩余油分布特征、以经济有效开发为目的的三次加密调整方法、原则和程序；制定了三次加密井初含水与可采储量测算方法；研究确立了扩大三次加密调整规模的途径。

In the second part, by using uniform design and orthogonal design methods, some groups of mix test of foam lightweight with different component are conducted, the test data is analyzed by neural networks model and regression model, and optimum mix proportion is obtained. Orthogonal design for mix material considering density factor is presented, as well as uniform design for mix material adapting to different density. The mechanic characteristics of foam lightweight material and engineering characteristics differing from general material under an allround press are founded. Through the analysis of asymmetry settlement for Doulong sluice and the comparison of correct deflection scheme, the foam lightweight material shows some advantages in the load reduction.

在泡沫轻质材料的研究方面，利用均匀设计和正交设计方法进行了几组不同组分的泡沫轻质材料的配方试验，利用神经网络模型和回归模型对试验数据进行处理，得出最优配方；提出了考虑密度因素的混料配方的正交设计方法和适合不同密度时的混料配方均匀设计方法；研究了泡沫轻质材料的力学特性，并发现了在围压作用下所表现出的不同于一般材料的工程特性；论文将上述研究成果用于斗龙港闸工程的处理设计，通过对该闸的不均匀沉陷分析和各种纠偏方案比较表明，采用泡沫轻质材料进行减载处理有一定的优越性。

Extraction technology of isoflavones in Trifolium pratense was studied by single factor and orthogonal experiment.

本文通过正交试验的方法，对结果采用Hplc检测，着重考察红车轴草异黄酮的提取各种试验条件，筛选出得率和纯度都较高的方法，以每次所得到的总异黄酮质量的多少来衡量方法的好坏。

The paper does experiment on the image restoration by using the regularized method with a variance uniformization constraint, and the results show that this method outgoes the Tikhonov regularized method.

本文利用带一致方差约束的正则化方法进行了图像恢复试验，试验结果表明该方法优于Tikhonov正则化方法。

At the same time, on the base of analysis of the present situation, the development trend and the leading edge technology, the necessity and feasibility about the exploitation of the full hydraulic bulldozer"s sate monitoring and fault diagnosis system is given; According to the structure, working feature and working environment, it works out system schema and system composition; The system is divided into display module, communication module and fault diagnosis module by means of block design, the implementation method and key parameter are also given; In light of the features of the full hydraulic bulldozer, the state monitoring and fault diagnosis platform composed of controller RC6-9 and display DI2/10 is designed based on CAN-Bus and the hardware design is completed; It lists the measured values and state of monitored parameters which should be real-time displayed in monitoring module as well as the data which will be transmitted; Furthermore, On the base of the study of, it gives 34 kinds of constant faults" diagnosis methods; Using BODAS software and DPS software, it compile the program code of the controller and the displayer; At last, this paper design and make the simulation test device of control system of construction machinery, through the experiment, the realizability, the categoricalness, the reliability, the stability and real-time are tested, in addition, the improved methods are given.

本文收集整理了国内外智能推土机研究的相关文献资料，综合分析了国内外智能推土机的研究现状、发展趋势和前沿技术，论证了开发全液压推土机智能系统的重要组成部分&全液压推土机状态监测与故障诊断系统&的必要性和可行性；根据全液压推土机的结构、作业特点和作业环境，制定了全液压推土机状态监测与故障诊断系统方案，确定了系统的结构组成；采用模块化设计方法，将整个系统分成了显示、通信和故障诊断三个模块，给出了各模块功能的实现方法，确定了各模块的关键技术参数：选用RC6-9和DI2／10作为系统的控制器和显示器，用CAN总线搭建了系统的通信平台，完成了系统的硬件设计；确定了系统监测参数和通信数据，对全液压推土机常见故障特征进行了分析，给出了34种常见故障的诊断方法；在BODAS软件和DPS软件的集成编程环境下，编写了控制器和显示器程序；设计并制作了工程机械控制系统模拟试验板，并在此基础上通过多次的程序调试与试验，对所设计的全液压推土机状态监测与故障诊断系统功能的可实现性、完备性、可靠性、稳定性和实时性进行了检验，证实了此系统的可行性，并提出了进一步的改进方案和措施。

Based on the noise theory and experi mentation ,the author founded the onli ne method to test current noise , validated the relation bet ween te mperature ,aperture and pressure with current noise .This paper firstly analyzed gener— ati ng mechanis mof discharge niose ,studied the measure and factor of discharge niose ,i ndicated an effective method for degradi ng the discharge noise ,which pro moted the performance of laser gyro fro m0.01°／h to 0.001°／h.

文中以氦氖气体激光器的噪声特性理论为指导，以试验研究为基础，建立了在线噪声电流测试方法，通过试验验证了温度、腔体毛细孔径、谐振腔充气气压等参数与放电噪声的关系，首次提出了放电噪声产生的机理，分析了电流噪声的检测方法及影响因素，给出了减小和抑制放电噪声的有效方法，使得激光陀螺的性能由0.01°／h 提高到0.001°／h。

At the end of the paper, based on the theory analysis, the results of tests in lab andthe design of test road, a new design method of the overlayer on the old PCC pavementis introduced, in that the influence of the interlayer in the overlayer is taken account.

最后，在以上的理论分析和室内试验结果基础上，本文对各种加铺层设计方法进行比较分析之后，结合试验路观测结果，提出了一种考虑反射裂缝的旧PCC路面上AC加铺层设计方法，并对该设计方法的进一步完善提出了设想。

And has done the tired life test of the hypoid gear. As the samples of the test data of gear life test is very few, the author has made the error model of tes tinghypotheses when using k-s method, the auther presented a testing hypotheses method which can make use of the information of the samples and reduce the error of probability Calculation. It is Called"the improved K-s method". The author has used this method to deal witch the life test datas and the result is identical with the result of the theoretical calculation.

为对锥齿轮进行疲劳寿命试验，结合其它科研项目，研制了多功能数控齿轮传动试验台，并在该试验台上以SC110锥齿轮为试件进行了疲劳寿命试验，针对齿轮寿命试验数据一般样本很少的情况，建立了用K—S法进行分布律假设检验的误差模型，并提出了一种能充分利用样本信息，大大提高检验精度的小样本分布假设检验的新方法——改进的K—S法，并用该法对寿命试验数据进行了处理，得到了与理论分析计算一致的结论。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。