试验方法

The results show that the test method is feasible and convenient on restriction of experimental conditions.

以镇胜公路平寨特大桥挂篮荷载试验为例，论述了采用等代千斤顶荷载进行挂篮预压试验的方案确定和结果分析，试验表明由于受试验条件限制采用此方法进行挂篮预压试验是方便可行的。

At the same time, 9 groups data are conversely choosed according to uniform orthogonal array from 71 groups observed data of suspended grid plunge pool, the mold is set up by the method of PPR, the surplus 62 groups data are used to proceed examination, the empirical type optimum design of experiments are proceeded inversely, all of these confirm that a lot of model experimental results can be replaced by a few of test times under nonstrict uniform experimental condition. The result can provide the feasibility reference basis for same kind of model design of experiments in future.

同时从71组悬栅消力池观测数据中，按均匀正交表反选9组数据，用PPR方法建模，剩余的62组数据进行检验，逆向进行了实证性优化试验设计，证实了即使在非严格的均匀试验条件下，仍然可以用少量的试验次数替代大量的模型试验结果，为今后同类模型试验设计提供可行性的参考依据。

The method of orthogonal design is applied in the DOE, and the experiments are simulated. The results are analyzed with the analysis of regression to study the effect of the drive and fuel economy performance by the factor of drive train parameters.

对动力传动系统参数进行试验方案设计，对试验方案进行动力性和基于循环工况的燃油经济性模拟计算，采用回归分析的方法对试验结果进行处理，研究了试验因子及其交互对试验指标的影响，并对参数进行了预测和优化。

There was no cross-reaction of the prepared raw antigen with the positive sera from dogs infected respectively with Ancylostoma caninum,Toxocara canis and Demodex canis and the highest titer of the positive serum from dogs infected with D.immitis was 1∶1024 detected by the dot-ELISA,which had good reproducibi-lity.

为快速诊断犬恶丝虫病，建立了犬恶丝虫抗体dot-ELISA检测方法，并与阻断试验、交叉反应试验、重复性试验、琼脂扩散试验和平行对照试验进行了比较。

The paper discusses the usefulness of the combined environmental test to air defense missile's reliability growth, the methods to define the test sections and environmental stresses and judge the faults, the methods to terminate tests ,and so on.

本文论述了综合环境试验对防空导弹可靠性增长的作用、试验剖面与环境应力的确定、试验中故障判决方法和试验结束方式等一系列试验中遇到的问题，并进行了探讨。

Objective To investigate extended-spectrumβ-lactamasesand AmpCβ-lactamases and metalloβ-lactamasesproducing by gram-negative bacilli in burns.Mehtods Adopitng double-disk synergy test,modified three-dimensional extract test and the enzyme inhibitor of EDTA-Na2to detect ESBLs and AmpC and mBLA producing strains.Using K-B test to perform the susceptibility testing.Results Among348strains of gram negative bacilli,ESBLs,AmpCβ-lactamases,ESBLs combined with ApmCβ-lactamases and mBLA produc-ing strains were found in122,47,9and20strains,the incidence being35.1%,13.5%,2.6%and5.7%respec-tively.

目的 了解烧伤感染主要革兰阴性杆菌产超广谱β-内酰胺酶、AmpC酶和金属β-内酰胺酶及其对常用抗生素耐药性情况，指导烧伤医院感染合理用药方法采用双纸片协同试验检测产ESBLs株，头孢西丁三维试验检测产AmpC酶株，头孢曲松三维试验检测同时产ESBLs和AmpC酶株，依地酸二钠协同试验检测产mBLA株，K-B法进行G -杆菌药敏试验。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

According to concrete conditions of the wing flap retraction-extension system of the 18 trainer aircraft, this paper ca nes out a research into the relibility demonstration test and the life test plans of the system, and deals with some key issues related to the test in a deep manner, such as relation between the reliability test parameters and the reliability indexes, converting from working time, composite test profiles, the mathod determining reliability test parameters for success ratio and so on.

本文针本对L8机襟翼收放系统的具体情况，研究了其可靠性验证试验和寿命试验方案，并深入研究了涉及试验的关键问题，例如：可靠性试验参数与可靠性指标之间的关系，工作时间与工作次数的折算，合成试验剖面，确定成败型可靠性试验参数的方法等等。

Moreover,it describles the advantages of using voltage series resonance method on GIS in site,and thus the performance of field testing de- vice developed by Guangdong Electric Power Research Institute and the test effect.

本文简要地叔述了 GIS 在现场安装后，进行耐压试验的必要性，耐压试验电压波形的选泽、标准及试验程序，并介绍了用串联谐振方法进行 GIS 现场耐压试验的优点，以及广东电力试验研究所研制成功的 GIS 现场耐压装置的性能和试验效果

Though geological structures in metallic ore areas are very complex and seismic method for ore exploration is still in experiment phase, potential of the method in the future ore exploration can be found from primary experiment effects of seismic method, as the method possesses larger prospecting depth and less ambiguity as well as higher precision to distinguish underground target.

尽管金属矿区的地质构造十分复杂，金属矿勘查中的地震方法技术还处于试验应用研究阶段，但由于该方法探测深度大、多解性少、分辨地下目标物的精度高，从该方法的初步试验效果就能看出地震方法在今后金属矿勘查中的潜力。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。