试验动物

Methods Guinea pigs were, to begin with, given MBP through the nasal route to induce the development of peripheral immune tolerance, and then immunized with specific antigen MBP plus complete Freund's adjuvant. The clinical symptoms of guinea pigs were investigated, and the erythrocyte rosette test, and lymphocyte blastogenesis test, the MBP-specific skin delayed-type hypersensitivity response and the levels of interlukin-4 (IL-4) and interferon-γ were also detected, and the pathological changes were examined as well.

先使豚鼠经鼻粘膜摄入MBP，诱导建立外周免疫耐受，然后用MBP加完全弗氏佐剂免疫豚鼠，观察动物的临床症状、E花环形成试验、淋巴细胞转化试验、迟发型超敏反应、细胞因子水平及病理改变。

Shown by GL-03 culture in vitro test, the ferment cultured milk has strong acid and cholate resistant ability, good adhesive, obvious inhibit to common pathogenic entero becteria, without drug tolerance factors, is a lactobacillus applicable to permanent planting of human body intestinal tract, and simultaneously the deterioration ratio of the cholesterol in vitro can be up to 56 percent; proved by the animal-test, the cholesterol-lowering cultured milk has the function of lowering serum cholesterol.

乳酸菌GL-03体外培养试验表明其有很强的耐酸、耐胆盐能力，粘附性好，对常见的肠道致病菌有明显的抑制作用，不携带耐药性因子，是一种适宜于人体肠道定植的乳酸菌，同时体外胆固醇降解率可达56％。动物试验证明降胆固醇酸乳具有降低血清胆固醇的功能。

Five models were used to investigate the "Changdong"" s anti-tumor effect: mice S180 sarcoma solid model, mice liver cancer H22 solid model, mice Lewis lung cancer model, as well as human liver cancer QGY and colon cancer LOVO implanted in naked mice; mice liver cancer H22 was also used to evaluate the enhancive effect of "Changdong" when it was co-applied with other anti-tumor agent or with radiotherapy; The "Changdong"" s cytotoxic effect in vitro on such tumors as QGY, LOVO, lung cancer A549, breast cancer MCF-7, as well as mice leukemia P388 was observed through MTT method. And investigate the effect of "Changdong" on proliferation of spleen lymphocyte, on activity of NK cell and on synthesis of IL-2 in mice bearing Lewis lung cancer, respectively. With propidium iodide straining, cell aigptosis and cell circle were analyzed by flow cytometry. Result:"Changdong" has an evident tumor inhibitive effect on mice tumor model as well as human tumor implanted in naked mice with a quantity-effect relationship.

使用小鼠S180肉瘤、小鼠肝癌H22实体瘤和小鼠Lewis肺癌模型，以及人体肝癌QGY和人体肠癌LOVO裸鼠异种移植模型，进行"长动"的抗肿瘤药效学研究：用小鼠肝癌H22进行"长动"合并放疗和化疗的增效作用研究；用MTT法进行"长动"对肝癌QGY、肠癌LOVO、肺癌A549、乳腺癌MCF-7、小鼠白血病P388等10株人体和动物肿瘤细胞的体外细胞毒性作用研究；用淋巴细胞转化试验、小鼠NK细胞活性试验、小鼠胸腺细胞增殖法分别测定"长动"对荷Lewis肺癌小鼠的脾淋巴细胞增殖的影响、自然杀伤细胞活性的影响和对小鼠产生白介素-2(IL-2)的影响；用流式细胞仪PI单染色法进行"长动"对人体肝癌QGY细胞的体外诱导凋亡作用和对肿瘤细胞周期的影响的研究。

To evaluate the safety of the DNA vaccine pVAX1/F1-V against plague, the purity of plasmid DNA pVAX1/F1-V was detected by SDS-PAGE and gelose electrophoresis method; pasmid DNA was detected by PCR in tissues of BALB/c mice immunized with the plasmids intramuscular injection.

为了研究鼠疫DNA疫苗pVAX1/F1-V质粒的安全性，试验运用SDS-PAGE电泳和琼脂糖电泳等方法检测了鼠疫DNA疫苗pVAX1/F1-V质粒的纯度，并以小鼠为动物模型进行了质粒pVAX1/F1-V的急性毒性和长期毒性试验，用PCR方法检测外源基因在小鼠组织中的分布情况，用ELISA、ELISPOT方法检测了其自身的免疫反应。

His experiments were conducted in his home, using very primitive improvised equipment and small experimental animals.

他的研究工作是在家时进行的，试验用的是小动物，试验设备十分简陋。

The operation had been preceded by many years of clinical and laboratory research and work on animal models.

手术前研究人员已经开展了多年的临床试验，并已在动物模型上做过试验。

The third part of this project is to evaluate the safety of the nutria gland. The assay items will include single dose acute toxicity, multiple dose subacute toxicity and mutagenicity test.

在安全性评估方面，本计画将进行北美巨水鼠腺体之动物毒性试验，含单一剂量急毒性及重覆剂量亚急毒性，以及基因毒性试验。

By 1960 an extremely rare type of birth defect was appearing across Germany. Babies were born with hands and feet attached directly to the body, a condition known as phocomelia.

导至这一悲剧的原因："反应停"在大鼠身上的通过的动物试验试验结果用于临床是有瑕疵的，大鼠身上有人所没有一种的酶，它使大鼠不会产生的海豹畸形儿。

Results: A decreased body weight was observed. Sucrose preference test showed that percentage of sucrose solution from the total liquid was decreased, which simulates anhedonia (i.e. a loss of responsiveness to pleasant events), a core symptom of depression and the defining feature of melancholia.

结果显示，模型动物体重明显降低；糖水偏好试验显示糖水摄入比例显著下降，这模拟了抑郁症的核心症状——快感丧失；旷场试验中大鼠水平移动格数、直立次数、理毛数明显减少，这分别体现了运动能力及社会交往能力下降、探索行为能力降低等。

Among the co-author, California Science students who Kawei A Ashrafi roundworm for the trial to object, because these animals and genetic characteristics similar to mammals, the test results also apply to humans.

论文的合著者、加州大学生理学者卡韦·阿什拉菲以蛔虫为试验对象，因为这些动物遗传特性和哺乳动物类似，因此试验结果也适用於人类。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。