试验动物

Methods Virus Cytopathogenic Effect was used in vitro to measure half inhibitary concentration (IC50), inhibitory concentration and treat index. Mouse influenzal viral preumonia experiment was carried out in vivo to observe the pulmonary pathologic change and calculate the pathologic rate and pathologic inhibition rate.

体外试验采用病毒细胞病变观察法测定药物的半数抑制浓度(IC50)和最小有效浓度及治疗指数；体内试验采用病毒接种致肺炎动物模型试验，观察肺病变并计算病变率和病变抑制率。

Methods:1DHBV test:by giving the poult duck DHBV by celiac can obtain DHBV model by which can be observed the effects of GanLe capsule.2 immunity function test:the effects of little mices on reticuloendothelium system,the form of serum hemolysin,and the weights of immunity organs.

鸭乙型肝炎病毒试验采用经腹腔接种制备的雏鸭DHBV血症模型作肝乐胶囊抗乙肝病毒的治疗观察。免疫功能试验：小鼠网状内皮系统吞噬功能的影响；血清溶血素的生成；免疫器官重量。结果：鸭乙型肝炎试验结果表明：用药0W、用药2W、用药4W、停药1W后动物血清检测DHBV DNA：除病毒对照组及肝乐胶囊小剂量外，肝乐胶囊中、大剂量组血清DHBV DNA均有显著或极显著降低(P〈0.05或P〈0.01)，并有量效关系。

Dr John Raff, CEO of Starpharma, said:"We are delighted to receive this very positive data supporting the safety of VivaGel in humans. Combined with our earlier animal studies demonstrating the effectiveness of VivaGel as an HIV preventative agent, we believe that this data supports future larger-scale efficacy and safety clinical trials of the product. VivaGel is shaping up to become an important weapon in the battle against the HIV/AIDS epidemic."

John Raff，Starpharma的CEO，他说："我们很高兴得到支持VivaGel在人体试验中安全性的正性数据，加上我们早些时候的所做的动物试验证实了VivaGel是有效的防爱滋病药，我们相信这些数据支持进行进一步大规模的该药药效和安全性的人体临床试验，VivaGel正顺利成为与爱滋病和透过性传染疾病传染作斗争的重要武器。"

To evaluate the toxicity of the sludge, dredged from Victoria Port of Hong Kong , to marine benthonic animals lived in its pouring region, the toxicity test of the sludge to marine benthonic animals, including benthonic shellfish and crustacean, has been conducted.

为了评价香港维多利亚港疏浚淤泥对倾倒海区海洋底栖动物的影响，进行了两种底栖贝类和一种底栖甲壳类的生物毒性累积试验。结果表明，试验底栖贝类结蚶和缢蛏暴露在试验淤泥中240h和480h后，随暴露时间的延长，累积率增大。

Methods Suspension quantitative germicidal test and animal toxicity test were used to carry out experimental observation.

方法采用悬液定量杀菌试验和动物毒性试验方法进行了试验观察。

It is involved in many aspects of organic matter decomposition, partial regulation of microbial activities, nutrient cycles and crumbly structure. Depending on their diet (saprophagous, phytophagous or predation), soil fauna is closely linked both to each other and to microorganisms, plants and soil.

本论文试图以土壤动物作为生物指标来评价土壤重金属污染，通过对重金属污染区(以浙江省富阳环山乡受污染的农田为例)土壤动物群落结构的调查和在室内设置一定的污染浓度梯度处理的盆栽试验两种方式研究了重金属污染对土壤动物群落的影响，主要结论如下： 1。

objective to understand and master the stachyose's function which is extracted from the fibre of plants.methods adopt the experimentation of animal function.results this product which is animal's defecation experimentation indicates that compare with the control group , there is obvious difference .

目的 了解植物提取膳食纤维水苏糖产品润肠通便功能情况。方法采用动物功能学试验。结果该产品经动物通便功能试验，体现小肠运动和排便功能与对照组有高度显著性差别。

Study the bioactivity of the n-HA/PA66 composite and the effects it would be to body's metabolism of calcium and phosphorus ion in vivo.(3) Study the osteo-conductivity and the ability to repair bone defect of the porous n-HA/PA66 composite and the feasibility use it as the scaffold of bone tissue engineering. Objects and Methods as follows: 1.To evaluate the biocompatability of nano-hydroxyapatite crystals and polyamide composite (n-HA/PA66) with the L929 cells.To proceed the morphological observation and take pictures of L929 cells after 1d,2d,4d,and 7d of co-cultured with extract of n-HA/PA66 ,and direct contact with n-HA/PA66.To determine light absorbtion value of every hole under 500 nm with enzyme linked immunity instrument after 1 d,2 d,4 d,and 7 d of contact of n-HA/PA66 extract with L929 cells,and direct contact with n-HA/PA66.In the meanwhile calculate the relative multiplication rate of cells,and evaluate them by six degree tests for cytotoxicity. To investigate the acute and chronic toxic reaction on the whole body induced by the new nano-hydroapatite crystals and polyamide composite(n-HA/PA66)after implanting in vivo and its effects on partial constitution of animal organs after implanting in vivo,and evaluate the potential and degree of subcuticular stimulation reaction.

本实验主要由以下三部分组成：一、n-HA/PA66 复合材料在动物体内、体外的生物相容性及生物安全性评价二、n-HA/PA66 复合材料植入动物体内的生物活性及近期对机体钙、磷代谢影响的实验研究三、网孔 n-HA/PA66 复合材料作为支架修复兔桡骨节段缺损的动物实验研究主要研究目标及方法如下：参照 GB/T16886.5-1997-ISO 10993-5：1992《医疗器械生物学评价细胞毒性试验体外法》之评价标准和要求，采用规定的 L929 细胞(小鼠结缔组织成纤维细胞)，分别经直接接触和材料浸提液与细胞共培养等方式对 n-HA/PA66 复合材料进行细胞毒性测试，采用细胞形态观察法观察两种细胞各组在 24h、48h、72h、5 天后各时相点的细胞形态学变化，并在显微镜下照相，从而对细胞与材料的生物相容性进行定性评价；同时采用细胞生长抑制法，以酶标仪定量测定评价各组 1，2，4，7 天 L929 细胞的相对增殖率，以定量测定并判别材料对细胞的毒性程度。

In order to verify that free radical damage and pulmonary surfactant deficiency may be two of ARDS major mechanisms, we used ARDS animal model to proceed to a prevention and treatment test of ARDS model by pulmonary delivery, results of animal study showed that the drug preparation can evidently reduced the mortality and pulmonary edema of model animals . Animal tests had showed that the drug preparation has no abnomal toxity.

为了验证自由基损伤和肺泡表面活性剂缺乏的结合可能是ARDS发病的主要因素以及制剂的疗效，通过建立ARDS实验动物模型对该制剂进行肺部给药防治ARDS的模型动物试验，实验结果表明能明显降低模型动物死亡率和肺水肿，同时，动物实验证明该制剂无异常毒性。

Animal experiments have already shown that, like humans, some respond differently to novel environments - and those who explore them are more likely to self-administer cocaine when given the chance.

动物试验已表明和人类一样有一些动物面对新环境会做出不同的响应--那些更多去探索这些新环境的动物们在一获得机会时自我摄入可卡因的可能性更大。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。