试验动物

This study was designed to investigate the effect of adlay soymilk on lipid metabolism in hamsters. Thirty male hamsters were used as experimental animals and divided into three groups. There are (1) control group,(2) 15% adlay soymilk (15% ASM) group, and (3) 30% adlay soymilk (30% ASM) group.

本研究探讨薏仁豆奶对仓鼠脂质代谢的影响，以30只雄性仓鼠为实验动物共分三组，分别是（1）控制组；（2）15%薏仁豆奶组；（3）30%薏仁豆奶组，试验共进行四周。

But this is only a guess, there haven't been epidemiological or animal study on this aspect at home and abroad by now. So we design the assay to study whether ethephon can effect physiological and behavior development of rat in adolescency, and to discuss the corresponding mechanisms.

因此，我们设计这次动物试验，初步研究乙烯利能否对青春期大鼠的生理、行为发育产生影响，并探讨其作用的相关机制，从而为进一步研究乙烯利对青少年生长发育的影响及其环境危险度评价提供初步实验依据。

This product can even be used by people allergenic to apitherapic products.

我公司所有产品均不在动物身上做试验，因为产品的所用成分大多取自天然。

According to the publication's first author, Dany Adams, Ph.D., Assistant Research Inestigator at the Forsyth Institute, applied electric fields hae long been known to enhance regeneration in amphibia, and in fact hae led to clinical trials in human patients.

依照该文章的第一作者、Forsyth的助理研究员Dany Adams博士的陈述，很久以前就知道应用电场可以增强两栖类动物的再生，实际上在人类患者已经开始了临床试验。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

［Objective］Antifatigue effect of American Ginseng Agent was studied by mice experiments.

［目的］通过小鼠动物试验验证西洋参制剂的抗疲劳作用。

METHODS:Anti inflamation, antipyresis,swimming test and phagocytosis of macrophage by diffusion method were used in this study.

依据药理实验方法学和有关规定作了抗炎、退热、游泳耐力、巨噬细胞吞噬功能的动物实验和体外抑菌试验。

Objective: To evaluate the protective ability of vaccinia virus vectored multi-epito pe live vaccine candidate for plasmodium falciparumin experimental anim als, and to lay foundation for next testing on Aotus sp.

摘 要 目的：探讨恶性疟原虫保护性抗原复合基因-痘苗病毒重组活疫苗候选株在实验动物的抗疟原虫攻击能力，为下一步进入夜猴及人体试验奠定基础。

The acute toxicity test indicated that OHSP of LD was big than 15000 mg/kg, it was showed that OHSP was safety and avirulent substance.

急性毒性试验中的LD50大于15000mg/kg，说明动物长期服用口腔保健精是安全无毒的。

Methods A total of 52 rats were divided into 3 groups, and the rat SCI model was established with Allen's method. After henterocoelia injection of the extract from leaves of ginkgo bilobe, the changes of hindlimbs locomator after SCI were observed with incline plane test and BBB scale.

60只大鼠分成3组，用Allen's改良法建立脊髓损伤的动物模型，通过Egb腹腔注射，用斜板试验法和Bosso， Beattie and Bresnahan评分法观察大鼠脊髓损伤下肢运动功能的变化。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。