试验

Carambola, after zymolysis,juicing and compositions regulation, was used for the contrast experiment of immobilized fermentation and free fermentation, the contrast experiment of immobilized fermentation under different temperature, and the optimized experiment of immobilized fermentation by multiple yeasts, respectively.

杨桃进行酶解取汁、成分调整后，分别进行固定化和游离发酵对比试验、不同温度下固定化发酵对比试验、复合酵母菌种固定化发酵优化试验。

In order to investigate buckling mode, buckling load and failure mode of structure, shear experiments were conducted for stiffened thin-walled composite plate. Finite element method was also used to analyze the buckling behavior of that composite structure. Analysis results reveal buckling mode and buckling loading of finite element analysis are accordable with experimental results.

通过对复合材料薄壁加筋板结构进行剪切载荷下的屈曲试验研究，得到结构的屈曲模态、屈曲失稳载荷以及破坏形式，并通过有限元方法对结构的屈曲进行数值分析，分析得到的复合材料薄壁加筋板结构的屈曲模态和试验结果一致，屈曲载荷与试验结果吻合较好。

To establish anaplasma artificial infection model in experimental animals which treated with dexamethasone to investigate the transmission route of the pathogen, mice were infected via intra-abdominal and subcutaneous inoculation with the blood from infected cattle, the homogenate of infected gadflies and viscera of infected mice, contacting infected mice, respectively, while rabbits were infected through co-feeding with affected cattle.

采用地塞米松注射液长期抑制试验动物的免疫力，通过小鼠腹腔、皮下接种含有无浆体病原的奶牛血液、阳性厩蝇内容物、脏器悬液，并进行阳性小鼠接触感染和兔子临床同层感染等试验，研究奶牛无浆体的感染和传播及在试验动物体内的消长规律，建立起无浆体人工感染模型，调查临床中病原体的实际传播途径。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Olaquindox,arsanilic acid,and oxytetracycline are three common kinds of veterinary drugs and feed additives.

采用斑马鱼、鲤鱼作为生物材料，通过斑马鱼急性毒性试验、胚胎发育试验和鲤鱼肾细胞单细胞凝胶电泳试验，分别从生物个体水平、细胞水平和分子水平，对兽药及饲料添加剂喹乙醇、阿散酸和土霉素进行了生态毒理学效应研究。

Using the computer-controlled electronic universal testing machine to do stretching test, compressing test and bending test, along veins on the bottom stalk of Arundo donax L., the stress-strain curves under certain experimental conditions were acquired and analyzed.

该文利用微机控制电子万能材料试验机对收割期的芦竹底部的茎秆进行了顺纹拉伸、压缩、弯曲试验，获得试验条件下顺纹拉伸、压缩、弯曲的应力－应变曲线，并进行了分析。

To evaluate the toxicity of the sludge, dredged from Victoria Port of Hong Kong , to marine benthonic animals lived in its pouring region, the toxicity test of the sludge to marine benthonic animals, including benthonic shellfish and crustacean, has been conducted.

为了评价香港维多利亚港疏浚淤泥对倾倒海区海洋底栖动物的影响，进行了两种底栖贝类和一种底栖甲壳类的生物毒性累积试验。结果表明，试验底栖贝类结蚶和缢蛏暴露在试验淤泥中240h和480h后，随暴露时间的延长，累积率增大。

Six kinds of superior Leguminosae and grasses including Sweet pea , partridge pea , Utah sweet vetch , meadow brome , yellow bluestem and cambar bluegrass are introduced from the Western and Middle parts of USA with similar climate to the Loess Plateau according to the theory of similarity of climate. The experiments are focused on adaptability, regularity of growth and development, eco-economic values, and technique and model of cultivation of the introduced plants, following the procedure of primary test, regional test and production test.

按照&气候相似论&的观点，选择与黄土高原生态条件较为接近的美国中西部地区，引进了多年生香豌豆、美丽鹧鸪豆、犹他甜苕子、牧场草、黄兰沙梗草、康巴早熟禾等6种优良水土保持草本植物，按照&初选试验—区域性试验—生产性试验&的程序，对引进植物的物候、抗性、生长发育规律、生态经济价值、栽培技术等进行系统研究。

All the isolates were first identified by different culture media including Niger seed agar. CACA agar, urease tests, ID32 identification kit, 37℃ growth test, and capability of forming capsule, and then the sequence of the LSUrDNA D1/D2 region were further analyzed.

分别以油菊籽、咖啡酸玉米琼脂、刀豆氨酸－甜菜碱－溴麝香草酚蓝培养基、尿素酶试验、37℃生长试验、ID32生化鉴定板和英膜形成试验等初步鉴定后，再经大亚基核糖体DNA的D1/D2区域序列分析进一步鉴定菌种。

The stress resistance of common roof garden plants, e.g. Dahlia hybrida, Callistephus chinensis, Calendula officinalis and Chrysanthemum multicaule were determined by mensurating such index as chlorophyll content, electrolyte leakage, water content, Pro content, MDA content and dissoluble sugar content. The result indicates that Dahlia hybrida is the best, Callistephus chinensis next, Calendula officinalis and Chrysanthemum multicaule are weaker as to drought resistance;while in the experiment on coldness resistance, the ability to bear coldness ranks from the best to the worst is Poa annua, Festuca arundinacea and Trifolium.

对小丽花、矮翠菊、金盏菊与黄晶菊等常见城市绿化植物进行的抗逆性试验研究，测定了叶绿素含量、膜相对透性、相对含水量、脯氨酸含量、丙二醛含量、可溶性糖含量等指标，结果表明：在抗旱性试验中小丽花的耐受性最强，矮翠菊次之，金盏菊与黄晶菊的耐受性较弱：抗寒性试验中所选植物材料的耐受性次序分别是早熟禾强于高羊茅强于三叶草。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。