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ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子,在许多同种的真核生物有它的同源基因,这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关,而这个家族的蛋白质和DNA的结合能,各种ARID蛋白质的专一性尽相同,过大致上偏好於和AT-rich的序结合;我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因,分别是garid1和garid2,我们首先对於garid1做分析;将AU1标记接到gARID1转染形鞭毛虫,用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下,过其阳性染色和蛋白质表现有明显增加;EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合,经由突变序分析,也显示gARID1的结合序为AGATC和AATAAAATA,随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

The artful Pelias welcomed hisnephew with false pride and joy ; he promised to give up the kingdom to one who had proved himself worthy of it.

他发誓说,他愿意放弃王位,但接替他继承王位的人必须能够用事实证明自己确配接受王冠。

This is enough to prove the falseness of such a concep

不要受汉语影响而用错不定冠词位置:这足以证明这种概念是不可靠的。

The generalized pivotal model is drawn by Fiducial inference, by which the generalized p-value is defined directly. Then the hypotheses test problem is solved and this test is proved to be valid at the boundary.

首先用Fiducial方法得到广义枢轴向量,由此直接定义广义p-值,并证明了由此广义p-值所给出的检验在边界上具有频率性质。

This demonstrated that flank wear and cutting speed had the most significant effect.

这个证明那胁穿用和尖刻的速度有最重要影响。

"But you have something to show that you are a freeman, haven't you?"

"那么您一定有其他可以用来证明您是自由身的东西吧?"

Applying Fresnel diffraction theory, this technique is proved to enlarge the equivalent dimension of CCD and increase the cutoff frequency of optical system, so this method can improve the imaging resolution of digital holography.

用菲涅耳衍射理论证明了该技术可有效增大CCD探测器的等效尺寸和系统截止频率,从而提高数字全息成像分辨率。

Wood packing materials fumigated with methyl bromide should be accompanied with an official certificate attesting to the treatment.

用溴甲烷熏蒸处理过的木质包装应附有官方证书证明进行了这种处理。

Wood packing materials fumigated with methyl bromide should be accompanied with an official certificate attest ing to the treatment.

用溴甲烷熏蒸处理过的木质包装应附有官方证书证明进行了这种处理。

Australian epidemic prevention Council by wooden cases of imports of goods, are required to provide proof of fumigation.

澳大利亚防疫局规定,凡用木箱包装的货物进口时,均需提供熏蒸证明。

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推荐网络例句

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47:5 法老对约瑟说,你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法,同时,采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。

Hey Big Raven, The individual lies dont matter anymore - its ALL a tissue of lies in support of...

嘿大乌鸦,个别谎言的事不要再-其所有的组织的谎言,在支持。