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Methods After intramuscular injection of 654-2 20 mg and perfusing rectal with warm nature water,we scaned 25 patients by CT which had been diagnosed as rectal cancer by operations or rectoscopy biopsy.

肌注山莨菪碱(645-2)20mg后,直肠灌注温生理盐水进行CT扫描,分析经肠镜取材病理证实及手术证实的25例直肠癌资料。

The control group is the nasopharyngeal wall of 55 healthy volunteer and 75 neck lymph nodes and 46 retropharyngeal lymph nodes of healthy volunteer.At last the results were compared and statistically analyzed.

在107例患者中共有51枚经穿刺证实为转移的颈部淋巴结及33枚经随访证实转移的咽后淋巴结,55例健康志愿者鼻咽壁及75枚颈部、46枚咽后淋巴结做对照组。

This paper also analyses the effect of water deficit on water use, yield, fruit quality, crop coefficient, water use efficiency and economical benefit, and achieved the followings:⑴The research of the water deficit treatment and rewater domino effect significantly test that proper water deficit can regulate and control stomal open degree efficiently, reduce the overtranspiration of leaves, stimulate the compensating effects.

更多水效应研究,进一步证实了适度水分亏缺可以有效调控梨枣树叶片气孔开度,降低叶片过多的蒸腾耗水,并刺激树体诱发补偿效应,初步揭示了调亏灌溉提高果树叶片水平水分利用效率的机理与水分亏缺引起的&干湿交替过程&对气孔的调控机理,从果树生理角度进一步证实了调亏灌溉的节水机理。

Methods A total of 196 cases of patients with scrotal disease were examined with ultrasound before operation or medicine treatment.

方法应用彩色多普勒超声对196例阴囊疾病进行检查诊断,30例急性附睾炎经治疗得以证实,余均经手术、病理证实

RESULTS: Cardiac amyloidosis was detected by EMB in 15 of the 33 patients. In patients with biopsy-proven cardiac amyloidosis, CMRI revealed a distinct pattern of late gadolinium enhancement, which was distributed over the entire subendocardial circumference, extending in various degrees into the neighboring myocardium.

结果:33例患者中,15例EMB证实有心肌淀粉样变性,在EMB证实患者中,CMRI显示延迟钆增强的特殊模式,分布于整个心内膜下,不同程度上延伸至邻近心肌。

Subsequently, a l kb fragment of HCCR-2 encoding region was amplified by RT-PCR and cloned into Promega TEasy vector.

测序证实无误后,以HCCR-2/T载体为模板,再通过PCR克隆出HCCR-2的编码区序列,将其连接到pIRES2-EGFP真核表达载体,并通过测序获得证实

Methods: total rna was isolated from hepatocellular carcinoma cell line hepg2. subsequently, a 1kb fragment of hccr 2 encoding region was amplified by rt pcr and cloned into promega teasy vector. after confirmed by dna sequencing, the full length encoding region of hccr 2 was amplified by pcr and cloned into pires2 egfp. the recombinant eukaryotic expression vector was confimed by dna sequencing.

从人肝癌细胞株hepg2提取rna,利用rt pcr方法,先克隆出一包括hccr 2编码区的长约1 kb的片段,并将其与t载体连接并转化,测序证实无误后,以hccr 2/t载体为模板,再通过pcr克隆出hccr 2的编码区序列,将其连接到pires2 egfp真核表达载体,并通过测序获得证实

METHODS: Total RNA was isolated from hepatocellular carcinoma cell line HepG2. Subsequently, a 1kb fragment of HCCR2 encoding region was amplified by RTPCR and cloned into Promega TEasy vector. After confirmed by DNA sequencing, the fulllength encoding region of HCCR2 was amplified by PCR and cloned into pIRES2EGFP. The recombinant eukaryotic expression vector was confimed by DNA sequencing.

从人肝癌细胞株HepG2提取RNA,利用RTPCR方法,先克隆出一包括HCCR2编码区的长约1 kb的片段,并将其与T载体连接并转化,测序证实无误后,以HCCR2/T载体为模板,再通过PCR克隆出HCCR2的编码区序列,将其连接到pIRES2EGFP真核表达载体,并通过测序获得证实

It has been well documented that the fibular collateral ligament is the primary varus stabilizer of the knee.8,10 Consequently, isolated FCL injuries can elicit abnormal varus knee instability that can lead to functional limitations, a varus thrust gait pattern, and the potential development of medial meniscal tears or medial compartment arthritis over time due to the increased compressive forces at the medial tibiofemoral compartment.16,23,30 In addition, it has been demonstrated that varus instability, primarily due to a deficient FCL from an untreated posterolateral knee injury, causes a significant increase in force on both anterior cruciate and posterior cruciate ligament reconstruction grafts.11,21,22 Therefore, a reconstruction technique to address nonrepairable acute or chronic isolated FCL tears is important to address these pathologic abnormalities.

已经证实腓侧副韧带是防止膝关节内翻的主要稳定结构[8,10]。因此,FCL离断伤可以引起异常的膝关节内翻不稳定,从而导致功能受限、膝内摆步态和潜在发展为内侧半月板撕裂或由于在增加胫股关节间隙的压力会随时间推移发生内侧间室关节炎[16, 23, 30]。此外,已经证实了主要由膝关节后外侧损伤后未经治疗导致FCL功能缺陷引起的膝内翻不稳可以引起前交叉韧带和后交叉韧带重建移植物受力明显[11,21,22]。因此,认为不可修复的急性或慢性FCL离断撕裂伤的重建技术对这些病理异常非常重要。

Therefore, fluorescein isothiocyanate conjugated lectins, namely concanavalin agglutinin, wheat germ agglutinin and soybean agglutinin, were used to localize lectin receptors on plasma membrane of male and female gametes and other relevant sexual cells, as well as embryos of various developmental stages of Nicotiana tabacum and Torenia fournieri by cooled charge coupled device and Confocal Laser Scanning Microscope.

从而证实:在本实验中用的几种酶组合对凝集素受体的影响并不大,即由酶解及解剖得到的雌性细胞的荧光在分布形式及强度上没有明显的差异;对比原位酶解与离体酶解得到的雌性细胞的荧光,证实机械操作对荧光的分布形式及强度也没有明显的影响。

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