计数

As the DDP group A549DDP cells were treated with IC10 of cisplatin for 48 hours. As the DDP＋Rh2 group A549DDP cells were treated with IC10 of cisplatin and IC5 of gensenoside Rh2 for 48 hours. As the controlling group A549DDP cells were not treated with any kinds of drugs. Changes in cellular morphology were observed by fluorescence microscopy. Peak value apoptosis of A549DDP cells were inspected by flow cytometry. State of PTP was evaluated by ultraviolate spectrofluorometer. Changs of calcium in cells and membrane potential of mitochondrion were determined by flow cytometry. Release of cyt-c from mitochondrion with westton blot Radiative activity of〓Tc-MIBI ingested by cells was measured withγcounter. Results: IC50 of cisplatine to A549 cells was 24uM.

以无毒浓度的Rh2单独作用于A549DDP细胞作为Rh2组，以低效浓度的顺铂单独作用于A549DDP细胞作为DDP组，以无毒浓度的Rh2和低效浓度的顺铂联合作用于A549DDP细胞作为Rh2+DDP组，作用时间为48小时，以不加药物干预作为对照组，Hoechst33258染色荧光显微镜下观察细胞形态的变化，流式细胞仪检测细胞凋亡峰；紫外光分光光度计检测线粒体PTP开放情况，流式细胞仪检测细胞内钙离子浓度及线粒体跨膜电位的变化，Westton blot检测线粒体内细胞色素C释放情况及Caspase-3的活性，γ计数仪检测细胞摄取〓Tc-MIBI放射性活性。

The results indicated:1 Some cognitive performances decreased obviously with age,such as symbol-digit, visual digit span, auditory digit span,pairedassociation learning,free recall of picture and recognition of meaningless figure But other cognitive performances did not decrease with age, such as picture classific...

结果表明：（1）一些作业（符号数字、视觉数字广度、听觉数字广度、词对联想学习、图象自由回忆和无意图形再认）成绩随年老明显下降，另一些作业（图象分类、卡片分类、心算和矩阵填图）成绩不随年老明显降低；（2）再次验证心算、符号数字、数字鉴别和计数四项非词语性作业速度随年老进行性减慢。

Methods The compound preparation was administered intragastrically in mice, whose spontaneous activity was observed along with their tolerance of the preparation after long-term administration. The hypnotic effect of the compound was assessed by investigating the changes in the pentobarbital sodium-induced sleeping. The compound was tested for its antiseizure effect in mice with pentetrazole-induced clonic and tonic convulsion. Diazepam was used as the standard control in all experiments.

利用光电计数法观察灌胃给予复方酸枣仁油栀子油对小鼠自发活动的影响，以及长期使用药物对小鼠耐受性的影响；利用延长戊巴比妥钠睡眠时间实验和戊巴比妥钠阈下催眠剂量实验，观察复方酸枣仁油栀子油的催眠作用；利用戊四氮诱发小鼠阵挛性、强直性惊厥观察复方酸枣仁油栀子油的抗惊厥作用；本实验用地西泮进行平行对照实验，并进行了组方设计和实验。

The test of white blood cell count was finished by the different number of bifidobacterium suspension coloclysis and general irradiation with 5.0 Gy γ ray to establish acute radiation damage pattern.The 30 survival rate was tested and reported after the bifidobacterium suspension coloclysis of 1×109CFU/ml and 5.0 Gy ray to establish acute radiation damage pattern number in every day.

WBC计数：用不同数量级浓度的双歧杆菌菌悬液对小鼠灌肠28 d后，5.0 Gy照射后3 d、6 d和10 d采取小鼠外周血检测WBC数。30 d小鼠生存率：用1×109CFU/m l双歧杆菌菌悬液灌肠28 d后，用一次性8.0 Gy60Coγ射线全身照射，建立急性放射损伤模型，每日上下午各观察小鼠1次，记录死亡动物数和死亡日期。

If we glance at the enumeration of the possibilities of the diverse variants program, then it is not difficult to note that the competitory solutions, the same as Download master.

如果我们一眼计数的可能性的不同类型节目那麼我们不难看到竞业禁止的解决方法，相同下载大师。

The platelet count, aggregation functions, adhesive ability, hypotonic shock reaction and CD62p expression of platelets preserved at normal temperature, cryopreserved on same day of collection and after storage for 3 days were detected, and possibility of using platelets cryopreserved after storage for 3 days in clinic was evaluated by comparison of contractable clinical cases.

对当天冷冻和保存3天后再冷冻血小板进行了计数，并检测聚集力、黏附力以及CD62p的表达，并通过可对比性病例观察保存3天后再冷冻与当天冷冻血小板临床应用的可能性。

After varied survival periods, the model mice were sacrificed to get the specimens of SN fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry to label the DA neurons and Calbindin D28k-containing neurons with the antibodies against tyrosine hydroxylase and CR respectively. The labeled cells were counted under microscope and analyzed statistically.

模型动物存活1周后，一部分取其中脑黑质节段，固定、包埋做连续冠状石蜡切片，以免疫组织化学染色方法，显示各组酪氨酸经化酶和钙结合蛋白表达阳性细胞，光镜观察并细胞计数，统计学分析；另一部分取其中脑黑质组织匀浆，行TH、CB的Westem blot免疫印迹方法检测，用LabWorks软件分析处理。

The model mice were then treated by giving GDNF into the striatum. After a seven-day survival period, the model mice were sacrificed to get the segment of substantial nigra fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry using anti -TH and anti-CB antibodies to label DA neurons and CB-containing neurons respectively, which were counted under microscope and analyzed statistically.

利用1-甲基－4-苯基1，2，3，6-四氢吡啶(1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine，MPTP)制备成年小鼠PD模型，通过单侧纹状体定位注射GDNF，取中脑黑质节段，做连续冠状石蜡切片，结合免疫组织化学染色方法，观察各组酪氨酸羟化酶(tyrosine hydroxylase，TH)、钙结合蛋白(calbindin D28k，CB)的表达，光镜观察并细胞计数，统计学分析。

Vibrio parahaemolyticus 1211U was cultured in sterilized sea water by the process of filtration at 4 ℃;50-60 days later, plate count and most probable number showed that there were no culturable bacteria.

将副溶血弧菌菌株1211U培养于低温贫养条件下，以平板菌落计数法和最大近似数法检测可培养细菌数，50～60d后表明可培养数下降为零。

Cells which secreting TNF-αwere measured by spot-forming cells under low power light microscope showed babyblue cyclitic spots.

ELISPOT方法检测到的斑点为淡蓝色圆形或者近圆形，大小不一，可以在低倍光学显微镜下计数，每个斑点代表一个分泌TNF-α的细胞。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。