计数

Here we investigate the effects of 17 β-estradiol (10〓～10〓mol/L) on the proliferation and differentiation of newborn rat calvaria osteoblastic cell.

对体外培养的成骨细胞施以不同剂量的17β-雌二醇，3天后通过细胞计数、〓—胸苷掺合、细胞定量后测定骨钙素及碱性磷酸酶的量观察对增殖和分化的影响。

Methods Rat calvaria osteoblasts were cultured in the presence of 10 μg/mL-500 μg/mL nHA or nAg-HA/TiO2 for 2 h, 6 h, 8 h, 24 h, 72 h and 120 h. Cell proliferation, vitality and ultrastructure were tested using cell counting chamber, MTT assay, and transmission electron microscope.

将成骨细胞分别在添加了不同浓度(10μg/mL~500μg/mL)的nHA或nAg-HA/TiO2的培养基中培养2、6、8、24、72及120h，通过透射电镜观察、细胞计数及MTT法检测细胞超微结构、细胞增殖及线粒体活性，并计算单位细胞呼吸率。

Retrieving capsulated cells for cell counting, MTT assay,CCK-8 assay daily for 8days to determine cell activity,and analysis growth curve.

囊管内细胞胰酶消化回收，以细胞计数、MTT法、CCK-8法测定细胞的活性，并进行生长曲线分析。

Impact of PCL capsulation on the mesenchymal stem cell activity studied in vivo, one plate culture with complete culture medium for control group with and two encapsulated transplantation for experimental groups(complete culture medium or basic culture medium for cell suspended) installed,after package and transplantation,retrieving capsulated cells for cell count,MTT assay,CCK-8 assay Daily for 8days to determine cell activity,and analysis growth curve.

以完全培养液平皿法细胞培养为对照组，设2个实验组进行聚己内酯囊管化骨髓间充质干细胞移植细胞活性研究。两个实验组分别以完全培养液和基础培养液悬浮细胞后封装移植，囊管内细胞回收后以细胞计数、MTT法、CCK-8法测定细胞的活性，共8天，进行生长曲线分析。

Methods: mvc and expression of tgfβ1 in 50 carcinomatous specimens and expression of tgfβ1 in 35 para carcinomatous specimens from the same 50 cases with nsclc were detected by sp immunohistochemistry.

通过应用sp免疫组化方法检测50例nsclc组织内微血管计数及50例nsclc组织和35例癌旁组织中tgfβ1的表达。

Methods Pathogenic bacteria from CSF were enriched by centrifugalization with high speed. Leukocytes were counted in microscope and the quantity of the protein was detected by automatic biochemical analyzer.

采用高速离心法浓集脑脊液中的致病菌，镜检计数定量脑脊液中的白细胞数，自动生物化学分析仪检测脑脊液中的蛋白量。

On single photon detection, in order to reduce the dead time and enhance the counting rate of single photon detector, a flexible and chronometrical circuit is

该技术使探测器工作在更加安全高效的模式下，并达到了提高SPAD计数率的目的。

MethodsMorphological change observation and live cell accounting were used to investigate the inhibitory effects of Cirsium japonicum Fisch.

方法细胞形态观察和活细胞计数法研究大蓟提取液对人白血病细胞K562，肝癌细胞HepG2，宫颈癌细胞Hela，胃癌细胞BGC823生长的抑制作用。

Methods: morphological change observation, live cell accounting and clone formation were used to investigate the inhibitory effects of Cirsium setosum MB on human chronic myloid leukemia K562 cell, hepatoma HepG2cell, cervical carcinoma Hela cell and gastric carcinoma BGC823 cell.

细胞形态观察、活细胞计数法和克隆形成率法研究小蓟水提液对人白血病细胞K562、肝癌细胞HepG2、宫颈癌细胞Hela、胃癌细胞BGC823生长的抑制作用。

By FCM analy sing, p53 gene can induce cells apoptosis and make it G1 arrest; Cisplatine can induce cell apoptosis and make it G2 arrest.

流式细胞计数证实p53能诱导QBC939细胞发生凋亡并导致其发生G1期阻滞，顺铂能诱导QBC939细胞发生凋亡并导致细胞发生明显的G2期阻滞。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。