计数

Using count the leave.and flow cytometric to identifiy ploidy levels for the regenerated plants derived from antherculture in burley tobacco in this article,it shows that using count the chloroplast method could identify the ploidy levels simply and economic but it cannot identify the mixoploid accurately, so it easily lead to misidentify.

本文分别用气孔保卫细胞叶绿体计数和流式细胞仪两种方法来鉴定花药培养再生植株染色体的倍性，结果发现叶绿体计数法可以简单经济的检测烟草花药培养再生植株的染色体倍性，但它不能准确地鉴定出混倍体植株，易造成误判。

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

Immunohistochemistrical section: observe the VEGF protein expression in different cells, the positive ratio detected by a morphometrical analysis system was used as the amount of the VEGF protein expression.

免疫组化染色切片：观察不同给药时间股骨头内VEGF的表达情况；高倍视野下（400倍）沿骨小梁表面计数50个成骨细胞中的阳性细胞数，VEGF成骨细胞阳性率=阳性成骨细胞数/50×100％，3次取平均值；计数每个高倍视野软骨细胞中的阳性细胞数，VEGF软骨细胞阳性率=阳性软骨细胞数/软骨细胞总数×100％，5次取平均值。

The positive correlation between volume and count in coincident counting and negative correlation between volume and count in noncoincident counting indicate that the sample's volume effect should be considered in counting of 32P water solution.

结果显示符合测量中体积与计数效率呈正相关关系，非符合测量中体积与计数效率呈负相关关系。提示在进行此类测量时，为了保证结果的准确可靠，须保持样品体积的一致或选用样品体积不敏感区间。

Methods Through 99mTcO4- nuclein imaging of thyroid to 321 patients with subacute thyroiditis.Dealing with imaging to compare the radioactivity count of jaw gland and the radioactivity count of thyroid.

对321例亚急性甲状腺炎患者进行99mTcO4-甲状腺核素显像，通过影像处理计算颌下腺放射性计数/甲状腺放射性计数的比值。

Some patients have ''monomicrobial bacterascites'' in which cultures are positive but there is normal ascitic neutrophil count.138 Such infections are thought to occur relatively commonly, and the majority are eradicated by the body's natural defence mechanisms (for example, opsonic and complement mediated bactericidal activity).138 139 When a positive culture is obtained, a further ascitic tap with a neutrophil count should be obtained.

有些患者有着&单一微生物细菌性腹水&，其腹水培养为阳性但腹水白细胞计数正常。138这些感染被认为较为常见，大部分可被机体天然的防御机制（如调理素和补体介导的杀菌活性）所根除。138，139当腹水培养为阳性时，应进一步进行腹穿并行嗜中性粒细胞计数。

Parameters of subjects in the two groups are provided for the symptom with fever, headache, pantalgia, fatigue, cough, chest pain, short breath, palpitation; and the test of white cell count,lymphatic cell count, platelet count, blood urea nitrogen, creatinine, alanine aminotransferase, aspartate aminotransferase, oxygen saturation, chest x-ray were taken.

观察两组SARS患者治疗前后的发热、头痛、全身疼痛、乏力、咳嗽、胸闷、呼吸困难、腹泻；白细胞计数，淋巴细胞总数，血小板计数，血尿素氮，血肌酐，谷丙转氨酶，谷草转氨酶；动脉血氧饱和度，胸部X线检查17项指标。

In this study we quantitatively evaluated, by a stable paralysis/stableinfusion rate method, the difference between two standardizedparalysis levels—train-of-four count of 2 responsesand posttetanic count of 2. Ten ASA physical status I–IIconsenting adult patients scheduled for elective surgery wereanesthetized (sufentanil/propofol), tracheally intubated, mechanicallynormoventilated with a fixed O2/air mixture, and normothermic;oropharynx and thenar temperatures were maintained above 36°and 32.5°C, respectively.

在本研究中我们用稳定肌松/稳定输注速度方法定量地评价了两个标准化肌松水平――四个成串刺激计数为2个反应与强直后计数为2――之间的差异。10例 ASA 分级 I–II 预定行择期手术的已获取同意的成年患者行麻醉、气管插管、用混合 O2/空气正常机械通气，且保持正常体温；口咽和鱼际温度分别维持在36°和32.5°C 以上。

Prestorage and poststorage leukocyte filtration apheresis platelets were transfused respectively to patients with malignancy accompanied by serious thrombocytopenia after chemotherapy. Peripheral blood platelet counts were made at 1h, 24h after platelet transfusion. The effectiveness was assayed by correct count increment. The incidence rates of FNHTR were explored.

46例肿瘤化疗后血小板减少患者，分别输注保存前过滤单采血小板与保存后过滤单采血小板，输注后1h及24h后检测外周血小板计数，以校正血小板计数增殖判定输注效果，并考查FNHTR发生率。

Dinger, who was one of the pioneers of quantum mechanics, imagined putting a cat, a flask of Prussic acid, a radioactive atom, a Geiger counter, an electric relay and a hammer in a sealed box.

&薛定谔的猫&是最著名的未证实实验之一。1935年，量子力学的先驱之一，艾尔文·薛定谔提出了一个妙想：把一只猫、一个装有氢氰酸的烧瓶、一枚放射性原子、一个盖格计数仪、一个继电器和一把锤子一起放置到一个密封的盒子中组成一个装置，如果放射性原子衰变，盖格计数仪将探测到放射性，之后向继电器发送信号使其断开，从而释放之前被固定的锤子，锤子会将砸碎烧瓶并释放其中的氢氰酸，盒子里的猫于是被毒死。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。