解冻

Subsequently,with the straw and the cell freezing tube,we usedone-step and two-step method to vitrify the embryo of Lats gene knockout mice.The development ratio reached 90% 90.1 % 89.2%, 91.4% and the implantation rauo reached 50.0%, 47.1% 43.4%, 46.5% respectively after thaw.

在此基础上，用冷冻麦管、细胞冷冻管分别采用一步法、二步法对Lats基因敲除小鼠胚胎进行玻璃化冷冻，胚胎解冻后，囊胚发育率分别为：90％、90.1％、89.2％、91.4％；移植后着床率分别：50.0％、47.1％、43.4％、46.5％，差异不显著；并且与对照组相比差异也不显著。

Before you warm her up...

在给她解冻之前。。。

You can divide them with wax paper and individual serving amounts so they're easy to separate and thaw.

你可以按照个人食用的量用蜡纸分好它们，以方便分装和解冻。

The toxicity of cryoprotectans increased as the concentration of its increased.3, the survival rate of embryos microinjected with 6M PM was 25.07±1.57% after being coolled in -20℃ for 10 minutes by programmed cooling method, whenas ,the survival rate of controls dealt with five steps balance method was 20.88±2.84%, which indicated that the chilling sensitivity of embryos with microinjection decreased.

而任何一种抗冻剂均随着浓度的增加其毒性随之增加。3、另外，在冷敏感实验中，显微注射6M的PM的胚胎胚胎，应用程序化法处理，以2℃/min的速率降至-20℃，平衡10min后解冻处理，发现注射PM的胚胎低温处理后成活率为25.07±1.57%，而6M的PM五步平衡法同步处理的对照组胚胎成活率为20.88±2.84%，说明显微注射的胚胎冷敏感性一定降低。

In the course of experiment,blanching time,temperature,as well as the way of color-preserving was studied especialy.

在试验中，以清水作热烫液，在不同的时间、温度条件下热烫后，通过测定黄花菜中V-c的含量、过氧化物酶的活力、解冻后的褐头率以及对黄花菜的质地的评价来确定最佳的热烫时间和温度。

The cantharellus cibarius freezing technics was studied through analyzing the freezing curve at different temperature,freezing speed and juice leakage by liquid nitrogen flash freezer .

采用液氮速冻，探讨在不同冻结条件下鸡油菌的冻结温度曲线及冻结温度与解冻后鸡油菌的汁液流失率之间的关系。

The facelift version adds new heated side mirrors, which defog/defrost quickly.

在改版的版本增加了新的加热侧镜，除雾/解冻迅速。

The diluent contain magnesium chloride, potassium citrate, sodium glutamate, dipotassium hydrogen phosphate, monopotassium phosphate and fructose, semen vitality is hightest after frozen.The average value is 0.52,highter than other 5 diluent significantly (P﹤0.01).So chose this diluent as semen cryopreservation diluent on chicken. Definite cryoprotectant, equilibrium time and thaw temperature:On the base of test one,research freeze sheltering effect about glycerin, DMSO ,DMA and DMF, each with 4 consistency.The result showed that 10%DMSO sheltering effect is the best, the next is 4%DMA,semen vitality are 0.58 and 0.55 after frozen.

冷冻保护剂、平衡时间和解冻温度的确定在试验一的基础上，研究甘油、二甲基亚砜、二甲基乙酰胺、二甲基甲酰胺4种冷冻保护剂在4种浓度下对鸡精子的冷冻保护作用，结果表明，10%DMSO保护效果最好，其次为4%浓度的DMA，精液冻后活力分别为0.58和0.55，两者之间差异不显著(P﹥0.05)。

The research of swelling dynamic behavior shows that the electrolytical ions in swelling media have a great effect on the swelling behavior of PVA gel and the equilibrium swelling ratio in physiological saline is less than that in aqueous media.

溶胀特性研究显示，PVA水凝胶在生理盆水中的平衡溶胀比小于其在蒸馏水中的平衡溶胀比，凝胶的平衡溶胀比随浓度和冷冻－解冻次数的增加而下降，其下降趋势满足幂函数的变化规律；水凝胶的溶胀过程符合溶胀动力学方程。

After that, the comparison of the fertilizable competences of ICSI and IVF on cryopreservated oocytes was studied in this article, in order to establish an optimized method to improve the fertilizable competences of oocytes up to the level of embryos.1 Effects of the reducing injury methods on IVF of cryopreservated oocytesThe result of the three methods, choosing the right time oocytes, using minimum size sample vitrification and culturing after thawing, showed that these ways could partly maintained the fertilizable competences of cryopreservated bovine oocytes.

减轻损伤方案对冷冻卵母细胞IVF的影响使用选择合适时期的卵母细胞进行冷冻；采用最小样本量玻璃化冷冻方法进行冷冻；在解冻后先孵育再进行体外受精等三种方案能够部分维持或恢复冷冻牛卵母细胞IVF受精能力。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。