角蛋白的

Objective: To study the differences of KAP6 - 1.2 for different kinds of goats, and to detect the expression of KAP6 - 1.2 mRNA on the adult and fetal skins of arbas cashmere goat.

目的：研究角蛋白关联蛋白KAP6-1.2对不同品种羊绒和羊毛在核苷酸和氨基酸水平上的影响及在山羊皮肤中的定位表达。

METHODS摘要: Otocyst epithelia surgically isolated from fetal rats were cultured and passaged. Proliferation and differentiation of cultured cells were observed using BrdU labeling to find the progenitors. Immunocytochemistry (Factin, cytokeratin and calretinin) was used to detect cell properties of cultured cells.

方法摘要：取孕鼠胚胎听囊细胞进行体外培养，用免疫细胞化学方法，分别对培养的细胞进行细胞角蛋白、F肌动蛋白和calretinin染色观察细胞特征；BrdU染色观察细胞增殖。

They were spindle-shaped, and showed positive reaction to antibodies against vimentin, and negative reaction to antibodies against keratin.

结果： 3种方法获得的细胞形态和生物学行为大致相同；波丝蛋白抗体染色阳性，角蛋白抗体染色阴性。

Using cytochalasin D to prevent global actin from forming filamentous structures, the production of Heliothis armigera nuclear polyhedrosis virus in Hz-AM1 cells grown in the medium containing 0.5μg/ml CD was completely inhibited while its production was reduced to 10〓 in the presence of 0.1μg/ml CD by plaque assay. However, Western blotting revealed that the actin quantity in infected host cells treated or untreated with CD was almost identical. Additionally, CD had no effect on the DNA synthesis of HaNPV.

Western blot表明细胞松弛素D并不影响受染细胞中肌动蛋白的含量，Dot blot表明病毒DNA的合成也没有受到影响，但电子显微镜超薄切片发现在0.5ug／mL细胞松弛素D中形成的病毒粒子形态与正常的有明显区别，认为细胞松弛素D抑制棉铃虫核型多角体病毒的增殖是因为抑制病毒的DNA和蛋白组装成完整有感染性的病毒粒子，即丝状肌动蛋白对病毒粒子的脱壳和DNA的复制、转录没有影响，但对病毒粒子的组装是必须的。

Methods Keratinocytes were isolated from the foreskins of children, and subjected to primary culture. The cultured keratinocytes were incubated with anti- keratin 16 antibody, or with isotype antibody as control, for various durations.

将抗角蛋白16抗体加入培养的角质形成细胞中，以同种型抗体作对照，研究外皮蛋白和Toll样受体2和4 mRNA表达。

The study of renal cell carcinoma was made in immunohistochemical expression of keratin and vimemin in 40 cases.

本文对肾细胞癌40例进行了免疫组化——角蛋白、波形蛋白表达的研究。

On the basis of light microscopic observation the immunohistochemical examination of CK,TG,NSE,vimentin and calcitonin was used to observe 9 cases of oxyphilic cell adenoma of the thyroid.

观察9例甲状腺嗜酸细胞腺瘤，在光镜观察的基础上，用免疫组织化学方法检测细胞角蛋白、甲状腺球蛋白、神经元特异性烯醇化酶、波形蛋白和降钙素。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Objective:(1) To compare the sensitivity, specificity, and positive prognosticative value in the diagnosis of rheumatoid arthritis by examining antifilaggrin antibody, anti-cyclic citrullinated peptide antibody and rheumatoid factor respectively simultaneously.

目的：①检测类风湿关节炎病人血清抗聚角蛋白微丝蛋白抗体、抗环瓜氨酸肽抗体及类风湿因子，比较单独检测和联合检测对RA诊断的敏感性、特异性、阳性预测值。

After purification, cells were cultured in Ham's F12 medium or Ham's F12 hormonally defined medium for different time respectively, and the specific expression of cytokeratin 18 (CK18) and alpha-1-fetoprotein in the primary cultures was characterized by indirect immunofluorescence method. Cell growth and proliferation was checked by MTT method.

观察培养细胞的形态特征，免疫荧光法检测胚胎肝细胞角蛋白18 (Cytokeratin 18， CK18)和甲胎蛋白(alpha-1-fetoprotein， AFP)表达， MTT法检测细胞活力和增殖能力。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。