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AIM To evaluate the mechanism that natural anti-keratin autoantibody inhibits the proliferation of keratinocytes and squamous cell carcinoma.

目的 探索天然抗角蛋白自身抗体对培养角质形成细胞及鳞癌细胞凋亡相关蛋白的表达影响。

METHODS: Otocyst epithelia surgically isolated from fetal rats were cultured and passaged. Proliferation and differentiation of cultured cells were observed using BrdU labeling to find the progenitors. Immunocytochemistry (Factin, cytokeratin and calretinin) was used to detect cell properties of cultured cells.

取孕鼠胚胎听囊细胞进行体外培养,用免疫细胞化学方法,分别对培养的细胞进行细胞角蛋白、F肌动蛋白和calretinin染色观察细胞特征;BrdU染色观察细胞增殖。

Methods Resazurin and MTT assay were used to detect the effects of scorpion tegument water-soluble protein, scorpion tegument keratin protein and STP component Ⅱ on the transformation of T and B lymphocytes, and NK cell activity in normal mice.

采用刃天青和MTT法检测蝎皮水溶性总蛋白、总角蛋白及水溶性蛋白组分Ⅱ对正常小鼠T、B淋巴细胞转化、NK细胞杀伤活性的作用。

In this paper, Attacus ricini nuclear polyhedrosis virus was studied. The molecular weight and pi of polyhedrin and structural polypeptides were determined by vertical slab SDS-PAGE and thin-layer isoelectrofocusing electrophoresis. The restriction pattern of ArNPV DNA was assayed by 5 restriction endonucleases.

本文较为系统的研究了蓖麻蚕核型多角体病毒(Attacus ricini nuclear polyhedrosis virus,简称ArNPV)形态特征和生化特性,并预测了多角体蛋白的二级结构,为开发利用蓖麻蚕核型多角体病毒这一生物资源提供了理论参考。

Double positive rate was about 80%. Albumin increasingly expressed following induction, but alpha-fetoprotein decreasingly expressed. Simultaneously, c-kit mRNA levels were significantly reduced. No significant changes were found in keratin-19 levels.

细胞诱导后肝细胞标志物白蛋白表达明显增加,而作为不成熟肝细胞的经典标志物甲胎蛋白表达减少,同时干细胞标志物 c-kit mRNA水平也明显下降,角蛋白19水平无明显变化。

It is a cell of ectodermal origin that has the specialized function of producing keratin, a complex filamentous protein that not only forms the surface coat of the epidermis but also is the structural protein of hair and nails.

它起源于外胚层,有特殊分化的产生角蛋白的功能,角蛋白是一种复杂的丝状蛋白,不仅能形成表面包膜,而且是毛发和指甲的结构蛋白。

METHODS: The different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育,倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数;将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育,分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

Keratin antibody IgM 3B4 specifically against Candida albicans in vitro. METHODS: The different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal anti??keratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti??

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育,倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数;将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育,分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

Sheep tibetan antelope by wool horn albumen the composition of amino acid and goat, sheep by wool fibre identical; but there is certain difference on the content scale of all sorts of amino acid, acid content of ammonia of group of cashmere tibetan antelope is 5.88%, all the acid content of ammonia of content; guang of acid of ammonia of the group in prep above cashmere wool and continous abb is 10.60%, alkalescent and amino acid and acidit

藏羚羊被毛角蛋白氨基酸的组成和山羊、绵羊被毛纤维相同;但在各种氨基酸的含量比例上有一定的差异,藏羚羊绒组氨酸含量为5.88%,均高于山羊绒和绵羊毛中组氨酸的含量;胱氨酸含量为10.60%,高于其它所测山羊的绒和毛;碱性氨基酸和酸性氨基酸含量接近(14.87%,15.23%);藏羚羊绒促螺旋蛋白含量低于降螺旋蛋白。

Effects of keratin powder on soil and leaching ratio of slow-release fertilizer are tested.

结果表明,变性后角蛋白粉料比未经处理的角蛋白原料易于降解。

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