角蛋白的

Immunohistochemical studies were performed in 6 cases. Cytokeratin 7 was diffusely positive in all cases studied, whereas cytokeratin 20 and cytokeratin 17 were focally positive in 4 and 2 cases, respectively.

我们给6例做了免疫组化，细胞角蛋白7全呈弥漫的阳性，细胞角蛋白20和细胞角蛋白17分别在4例和2例中呈局灶阳性。

The result of clustering analysis showed that the relationship between Alauda arvensis and Melanocorypha mongolica was closer than others; the relationship between Eremophila alpestris and Calandrella cinere was closer than that between Eremophila alpestris and Calandrella cheleensis. But the relationships between the five birds and Galerida cristata were all far.

羽毛角蛋白凝胶电泳是在生物大分子的层次上，对6种百灵鸟的正羽羽片、绒羽和正羽羽茎角蛋白进行SDS-PAGE，聚类分析表明：云雀和蒙古百灵的亲缘关系较近；角百灵与短趾百灵亲缘关系最近，与亚洲短趾百灵的亲缘关系次之；而凤头百灵与这五种百灵的亲缘关系均较远。

The relationship of six species from Alaudidae (Alauda arvensis Melanocorypha mongolica Calandrella cinerea Calandrella cheleensis Eremophila alpestris Galerida cristata) distributed in Inner Mongolia were studied from three aspects: Mostly in feather keratins, chromosomal karyotype and morphology adding.The contour feather vance, down feather and shaft of six Larks were studied by means of SDS-PAGE of feather keratins.

本文在羽毛角蛋白、染色体核型及形态学三个层次上，以羽毛角蛋白凝胶电泳分析为主，以染色体核型分析和形态学分析为参考，对分布于内蒙古地区的百灵科Alaudidae的6种百灵鸟的亲缘关系进行研究，包括云雀Alauda arvensis、蒙古百灵Melanocorypha mongolica、短趾百灵Calandrella cinerea、亚洲短趾百灵Calandrella cheleensis、角百灵Eremophila alpestris、凤头百灵Galerida cristata。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示：未妊娠时，泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达，波形蛋白在子宫内膜基质细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，牦牛子宫任何部位均不表达角蛋白7；妊娠30天左右时，泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达，波形蛋白在子宫内膜基质细胞和内胚层细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，角蛋白7在尿囊细胞内表达，偶尔在腔上皮细胞的细胞核边缘表达；消化法进行原代培养时，组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞；分离得到的子宫内膜基质细胞活率达90%以上，并可在体外传代7次以上；分离得到的子宫内膜腺上皮细胞活率可达85%以上，并可在体外传代5次以上；RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长，维持子宫内膜基质细胞正常生长的FBS添加量为20%，维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

The result of homological analysis showed that an open reading frame,which contains 289 amino acids,is more similar to the Choristioneure fu- m...

比较多种核型多角体病毒的多角体膜蛋白和多角体蛋白的氨基酸序列发现，这两种多角体结构蛋白的同源性具有相似的变化趋势。

And the different concentrations of natural monoclonal anti-keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti-keratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.

结果：与对照组比较，不同浓度的单克隆天然抗角蛋白抗体IgM3B4均能抑制白念珠菌芽管生成，并且抗体浓度越高，抑制白念珠菌芽管形成的作用越明显；同时单克隆天然抗角蛋白抗体IgM3B4能够显著减少白念珠菌与角质形成细胞、上皮细胞、内皮细胞的粘附，其抑制作用与单克隆天然抗角蛋白抗体IgM3B4的浓度呈正相关。

Keratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein. RESULTS: It was found that the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 could inhibit Candida albicans germ tube formation and adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.Its inhibitory action was positively related to the level of natural monoclonal anti??

结果：与对照组比较，不同浓度的单克隆天然抗角蛋白抗体IgM 3B4均能抑制白念珠菌芽管生成，并且抗体浓度越高，抑制白念珠菌芽管形成的作用越明显；同时单克隆天然抗角蛋白抗体IgM 3B4能够显著减少白念珠菌与角质形成细胞、上皮细胞、内皮细胞的粘附，其抑制作用与单克隆天然抗角蛋白抗体IgM 3B4的浓度呈正相关。

Finally confirmed that,The thighbone and the osteoporosis morbidity is possibly connected protein 6, respectively are: The lactoferrin light chain, membrane association protein A3, the enolase, ATP gather the enzyme, the acetyl coenzyme A reductases, the myo calcium protein; The lumbar vertebra and the osteoporosis morbidity is possibly connected protein 5, respectively are: The actin, the keratin, the enolase, ATP gather the enzyme, the myosin; The thighbone and the strong bone valuable curative effect is possibly connected protein 9, respectively are: The enolase, ATP gather the enzyme, the myo-calcium protein, the creatine activating enzyme isozyme, the phosphoglyceric acid change flavor the enzyme, the myosin, the lactoferrin light chain, the pyruvic acid activating enzyme isozyme, the crown protein; The lumbar vertebra and the strong bone valuable curative effect are possibly connected protein 8, respectively are: The carbonic anhydrase, the actin, the αB-crystal protein, 3-phospho-glycerol aldehyde oxidase, the serum albumin, ATP gather the enzyme, the myosin, the enolase.

最后确认：股骨与骨质疏松发病可能相关的蛋白6个，分别为：乳铁蛋白轻链、膜联蛋白A3、烯醇化酶、ATP合酶、乙酰辅酶A还原酶、肌钙蛋白；腰椎与骨质疏松发病可能相关的蛋白5个，分别为：肌动蛋白、角蛋白、烯醇化酶、ATP合酶、肌球蛋白；股骨与强骨宝疗效可能相关的蛋白9个，分别为：烯醇化酶、ATP合酶、肌钙蛋白、肌酸激酶同工酶、磷酸甘油酸变味酶、肌球蛋白、乳铁蛋白轻链、丙酮酸激酶同工酶、冠蛋白；腰椎与强骨宝疗效可能相关的蛋白8个，分别为：碳酸酐酶、肌动蛋白、αB-晶体蛋白、3-磷酸甘油醛脱氢酶、血清白蛋白、ATP合酶、肌球蛋白、烯醇化酶。

Keratinase can degrade disulfide bonds in the keratin obviously,and make it hydrolysable,which can effectively solve the problem of serious pollution in the traditional unhairing process in leather-making industry.

角蛋白酶可以特异降解维系角蛋白结构的双硫键，使角蛋白发生水解，从而达到使动物皮脱毛的目的，因而能够从根本上消除传统制革工业硫化碱脱毛产生的严重污染。

There weer significant difference between between the AKA positive and negative patients.Conclusion: AKA is a valuable diagnostic tool for EORA,especially for early EORA.

血清抗角蛋白抗体可能是一种诊断老年类风湿性关节炎特异性抗体；血清抗角蛋白抗体阳性且具风湿性多肌痛症状的患者也许是早期老年类风湿性关节炎患者。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。