角膜镜

Initially, a person with early stage keratoconus will notice a subtle change in their vision.

最初，一个早期的圆锥角膜患者经常出现视力的细微改变，随着疾病的发展，视力将出现波动，近视镜和角膜接触镜的度数经常改变。

This experiment investigate the expression of TGF- B1 and bFGF in the rabbet corneas to find whether such expression contribute to the corneal wound healing after PRK; and compare the effects of mechanical deepithelialization with chemical deepithelialization PRK on the corneal wound healing.

裂隙灯显微镜下观察角膜Haze及角膜上皮愈合、水肿消退的情况；光镜下观察角膜组织病理学变化并计数不同时间点角膜基质浅层角膜细胞数量的变化；用逆转录-PCR法及免疫组化法检测正常对照组及PRK手术组术后1天、1周、1月、3月不同时点角膜TGF—β1和bFGF的表达情况。

In this review, the research progress in polymeric hydrogels based on poly-HEMA, organic silicane, molecularly imprinted polymer and ion ligand-containing polymer for sustained ophthalmic drug release and soft contact lens is reviewed, and their advantages/drawbacks are critically analyzed.

综述了基于角膜接触镜、适于缓释眼用药物的poly-HEMA水凝胶、硅氧烷水凝胶、分子印迹聚合物水凝胶以及含离子配体水凝胶的研究进展，并展望了适于角膜接触镜缓释的眼用制剂的水凝胶材料的发展前景。

Methods 500 normal cases (918 eyes), 200 long term soft contact lens wear cases (386 eyes) and 100 long term RGP wear cases (195 eyes) were enrolled in this study.

应用超声角膜测厚仪检测无角膜接触镜配戴史者500例（918眼）、长期持续配戴软性角膜接触镜2年以上200例（386眼）和长期配戴RGP≥1年者100例（195眼）的中央角膜厚度。

The onset of EGFP fluorescence and its distribution in the Wistar rat corneas were determined through fluorescence stereoscope.

建立大鼠异体角膜移植模型，用含rAAV1-EGFP转染液的培养基在37℃孵育SD大鼠角膜植片同时浸泡缝线18h，再用此缝线间断缝合，将植片移植到大鼠角膜植床，术后通过荧光体视镜观察Wistar大鼠角膜出现EGFP荧光的起始时间及分布情况。

Methods A lamellar flap was produced in the rabbit cornea with a microkeratome. The flap interface in whole corneas and corneal beds were soaked in the rAAV –EGFP solution for 10 min, BSS solution was applied in the control group. The corneas were harvested at day 1, 3, 14, and 21 after application for the structure analysis of cornea by HE staining. Expression of the transferred gene was monitored by fluorescence stereoscope and laser confocal microscope. Results After 3d of infection with rAAV-EGFP ,slight fluorescence was detected in rabbit cornea by fluorescence stereoscope .

微型角膜刀制作兔角膜基质瓣；转染组用含rAAV－EGFP转染液浸泡角膜瓣和角膜基质床10 min，对照组用等量BSS液浸泡角膜瓣和角膜基质床10 min，不同时间点(1、3、7、14、21d)通过荧光体视镜观察角膜出现EGFP荧光的起始时间及分布情况，收集角膜，其中一半用激光共聚焦显微镜观察、照相，另一半固定后行HE染色。

This article summarized the influence factor of ametropia after PKP and the main treating methods, including wearing on the glasses or adhesive glasses, taking out stitches of cornea selectly, loose the cut, etc.

对影响PKP术后屈光状态的因素及其主要矫治方法如配戴框架眼镜或角膜接触镜、选择性角膜拆线、松弛性切口、散光性角膜切削术、角膜楔形切除术、放射状角膜切开术、准分子激光手术等进行简要综述。

Methods. PSF 1000 analyzer was used to measure retinal image quality for eye of 26 subjects (spherical ranged from –3.00 ~-6.00Ds, astigmatism ranged from –0.75~-3.00Dc) fitted with three forms of full correction in random order : unaided eye, with rigid gas permeable contact lenses and toric soft contact lenses. The modulation transfer function curve was observed, 12 points of the MTF curve ( equivalent 12 points of LogMar VA) was used for analysis. Results.

利用人眼点扩散函数分析系统（PSF-1000）检查和评估26例近视散光患者（年龄18~28岁，球镜–3.00DS~-6.00 DS，角膜散光-0.75DC ~-3.00DC）在裸眼、配戴散光软性角膜接触镜和透氧性硬性角膜接触镜三种全矫状态的光学成像质量，采用26眼屈光介质MTF曲线中的12个点（即分别等同于对数视力表4.0~5.1的12个点）进行比较和统计学分析，为了避免主观误差，三种矫正方式的先后顺序随机选择。

00Ds, astigmatism ranged from ?0.75~-3.00Dc fitted with three forms of full correction in random order : unaided eye, with rigid gas permeable contact lenses and toric soft contact lenses. The modulation transfer function curve was observed, 12 points of the MTF curve ( equivalent 12 points of LogMar VA) was used for analysis. Results.

3.00DS~-6.00 DS，角膜散光-0.75DC ~-3.00DC）在裸眼、配戴散光软性角膜接触镜和透氧性硬性角膜接触镜三种全矫状态的光学成像质量，采用26眼屈光介质MTF曲线中的12个点（即分别等同于对数视力表4.0~5.1的12个点）进行比较和统计学分析，为了避免主观误差，三种矫正方式的先后顺序随机选择。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。