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One group was cultured in DEME free of serum; the other in DEME with 10 μg/ml lipopolysaccharides for 6 hours. NF-κB was studied by flow cytometry technique using immunofluorescent staining, and by gel shift assay using γ-32P labeled NF-κB oligonucleotide probe.

取体外培养的第2或3代人角膜基质细胞,同步化后分为2组:无血清的DMEM液培养的不加药组;含质量浓度为10 μg/ml脂多糖的DMEM液培养6小时的加药组。

Objective:Since some limitaions of SRK Ⅱ formula,the dual cubic curved regressive formula was designed. methods:By means of computer,statistical analysis and regressive treatment of kerato diopter,axial length and emmetropic IOL diopter of 415 pseudophakia was done.

针对目前临床上常用的SRKⅡ公式在计算人工晶体屈光度时的局限性,对415只后房型人工晶体眼的角膜屈光力、眼轴长度和正视化人工晶体屈光度进行回归分析后,设计出一种不同于传统的线性回归公式的曲线回归公式——二元三次计算公式。

In noninfectious uveitis (28.3%), stippled, globular, and multiple globular types of KPs were found.

非感染性葡萄膜炎(28.3%)的角膜后沉着物的特征是斑点状的、球形的以及多球形的。

Primary culture of bovine corneal endothelial cells and whole rabbit corneas were used as the experimental materials. We first treated the cultured bovine corneal endothelial cells with PTPs inhibitor, sodium orthovanadate, with a variety of concentrations (25,50,100μM) for various durations (8,24 hrs). The effects of PTP inhibition on cellular distribution of cell-cell junctional proteins, such as N-cadherin, alpha-catenin and p120, were evaluated by immunohistochemical staining with fluorescein microscopy and confocal microscopy. Immunohistochemical staining with Ki67 Ab, a marker of cell proliferation, was also used to detect cells entering cell cycle.

实验材料为初代培养之牛角膜内皮细胞和全厚度之兔子眼角膜,使用sodium orthovanadate作为酪胺酸磷酸脢的抑制剂,接著以不同SOV浓度(25, 50, 100μM)及不同时间(8, 24 hrs)来处理初代培养之细胞与新鲜的兔子眼角膜,之后进行萤光染色并使用萤光显微镜及共轭焦显微镜进行影像撷取,以观察位於细胞与细胞间的蛋白质,例如:N-cadherin, alpha-catenin 和p120的改变,另外也使用Ki67抗体来侦测是否有进入cell cycle的细胞。

As the laser reshapes the cornea, it changes the angle at which you see images and therefore strengthens your vision significantly.

随着激光reshapes角膜,它的变化的角度上你看到的图像,从而大大加强了您的视力。

Astigmatism is caused by irregularities in the surface of the cornea or lens and causes the image on the retina to be distorted.

散光所造成的不正常现象的表面角膜或晶状体和原因的形象在视网膜上的扭曲。

This is because the eyeball is too short, causing the light rays around objects to travel behind the retina, because they havent been bent enough by the cornea and lens.

这是因为眼球的是时间太短,造成光线周围物体的旅行背后的视网膜,因为他们haven 吨执意不够的,由角膜和晶状体。

HSV-I is the primary virus which leadings to cornea! endotheliitis.2. By using the PCR to detect HSV-I DNA in humor of the comeal endotheliitis is rapid and simple for etiologic diagnosis and is convient for clinical treatment.3. The conclusion is limited to diagnosis of HSV and VZV. It is necessary to do further for the other pathogeny.

P CR技术检测角膜内皮炎患者房水中的单纯疤疹病毒I型,可在分子水平上建立一种快速准确的诊断,从而从基因内部探测其发病原因,为临床治疗提供可靠的依据;兔膜内皮炎主要是以HSV一I感染为主;本研究未设计其它相关病毒的特定引物进行特异性扩增,故所得结论仅限于单纯疤疹病毒及带状疤疹病毒,是否有其它感染原,需进一步研究。

JMA not only brings into play importance rules of qi-benefiting and surface-defending to effect a permanent cure on protection function of eye that gives attention to heat-clearing and toxin-removing to remove evils for temporary solution, it also treats both principal and secondary aspects of the disease, which really embodies a basic academic viewpoint of traditional Chinese medicine that"Fu Zheng Qu Xie" under the wholistic concept of Chinese medicine can serve to prevent and cure HSK recurrence.

角膜安不仅发挥了组方中益气固表以扶本护目之整体功能的作用,而且兼顾了清热解毒以祛邪治标的作用,祛邪与扶正同施,标本兼治,充分体现了中医药整体观指导下的&扶正祛邪&防治HSK复发的基本学术观点。

Usually the shape of the cornea and the eye are not perfect and the image on the retina is out-of-focus or distorted.

通常的形状,眼睛的角膜和不完美的形象和在视网膜上的失焦或歪曲。

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