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Objective: To explore the relationship between corneal storage methods and rejection after corneal transplantation, and to study the roles of intercellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor-α in aqueous humor and serum in keratoplasty rejection in rats.

目的:探讨角膜保存时间与术后免疫排斥反应的关系,检测角膜植片的细胞间黏附因子-1(ICAM-1)表达及房水、血清中肿瘤坏死因子-α含量与角膜移植排斥反应的关系。

The animal experiment stage: 40 rabbits underwent corneal epithelium curettement and limbal ablation to induce limbal stem cell deficiency and were randomly divided into four groups, simple amniotic membrane group, non-frozen cells group, glycerol group and dimethyl sulfoxide group(n=10 for each group). Searching for the activity of frozen and non-frozen cells more completely by transplanting subculturing cells and observing the accomplishment of cells function.

动物实验阶段:将40只角膜缘干细胞损伤的模型兔随机分成单纯羊膜组、未冷冻细胞组、DMSO保存细胞组和甘油保存细胞组,每组10只,用传代培养2周后的角膜缘上皮细胞作移植实验,观察细胞体外功能完成情况来进一步比较深低温保存前后角膜缘上皮细胞的活性。

One hundred consecutive eyes with endothelial failure were entered into a prospective study of endothelial keratoplasty, and the donor central endothelial cell density was recorded postoperatively at 6 months (n = 98), 12 months (n = 96), and 24 months (n = 85) and then compared with the preoperative eye bank measurements.

实验方法:选取角膜内皮功能失代偿的100只眼作为角膜内皮移植术的前瞻性研究对象,分别于术后6月(n = 98)、术后12月(n = 96)、术后24月(n = 85)测量供体角膜中央内皮细胞密度,并与术前所测眼库的眼角膜中央内皮细胞密度值进行比较。

Methods Rat models of corneal isograft and corneal allograft were established, The submandibular lymph nodes were harvested before operation and at day 1, 3, 5, 7, 10, 14 after operation. The expression of CCL19 mRNA in draining lymph nodes were detected by reverse transcription- polymerase chain reation, and their chemotaxis to dendritic cells were examined in Boyden chamber.

建立大鼠正位穿透角膜移植模型,RT-PCR法测定正常角膜0d及角膜移植后1d,3d,5d,7d,10d,14d的局部淋巴结内趋化因子CCL19 mRNA的农达变化,应用Boyden小室进行不同时相淋巴结组织液对体外培养树突状细胞的趋化实验。

Purpose:To investigate the clinical effects of phototherapeutic keratectomy on the treatment of diffuse corneal foreign bodies caused by gunpowder explosion.

目的:探讨准分子激光治疗性角膜切削术在角膜浅层弥漫性火药伤中的应用,为角膜弥漫性火药伤提供一种新的有效的治疗方法。

Nubecula is one of the main causes to amblyopia, thus pediatric keratoplasty has great significance to the recover of visual function of these children.

角膜混浊是导致儿童不可逆弱视的主要原因之一,因此儿童角膜移植术对于角膜混浊儿童的视功能恢复具有极为重要的临床意义。

SIA degree in phacoemulsification with 3.2mm clear corneal incision has no connection with incision positions. Corneal curvature at incision meridian will be released, but another meridian has no distinct effect. The SIA analyzed by Jaffe/Clayman vector method will provide an important reference for toric IOL implantation surgery.

3.2mm透明角膜切口SIA的度数与切口位置无明显关系,切口对所在子午线角膜曲率有松解作用,对与切口垂直子午线角膜曲率无明显影响,矢量公式法计算所得的SIA值在散光型人工晶状体植入术中提供了重要的参考价值。

AIM:To investigate and analyze the central corneal thickness of hyperopic eyes and assess the safety of LASIK for hyperopia.

目的:应用角膜测厚仪测量和分析远视眼的中央角膜厚度,探讨LASIK治疗远视眼的安全性。方法:回顾性统计分析102例204眼远视眼的中央角膜厚度,并与激光切削深度做对比。

Intrastromal corneal ring segments (Intacs; Addition Technology Inc., Fremont, California) were designed to achieve a refractive adjustment by flattening the central corneal curvature while maintaining clarity in the central optical zone.

角膜基质环植入术(Intacs,Addition Technology Inc。, Fremont,加州)」的设计是利用拉平角膜中央的弧度,来维持角膜中央视力清晰,达到调整折射、改善视力的目的。

Ultrasound pachymetry was also measured preoperative.2.3 Observational componentThe changes of naked vision, best corrected vision acuity, intraocular pressure, corneal thickness and power, irregular and difference of the posterior comeal surface, and the simulated keratoscope reading of the anterior cornea were evaluated.2.4 Statistical AnalysisData were processed with SPSS 10.0 software, each group data were analyzed by Independent-Samples t Test and multiple regression analysis.

统计分析方法使用SPSS10.0 软件进行数据统计分析,各组数据之间比较采用Independent-Samples t检验和多元回归分析。三、结果LA81K术后角膜后表面的变化 l、LASIK术后角膜后表面 Orbsca川图形的变化角膜后表面相应于前表面切削区的部位可看到有区域变红。

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