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The animal models with limbal stem cell dysfunction were made by limbus region resection combined with curettement of the corneal epithelium.

以新西兰白兔为实验对象,采用角膜缘切除联合角膜上皮刮除的方法制作兔角膜缘干细胞缺乏的模型。

The corneal suface of children with emmetropia has toric shape. There is a fine coincidence between two methods in central corneal power. The corneal surface of children with emmetropia has the feature of irregularity and non-spherical surface.

儿童正视眼角膜表面呈托力克光学面;角膜曲率计与角膜地形图仪对于中央区角膜曲率测量有很好的一致性;儿童正视眼角膜表面具有一定的不规则性(CIM约0.63)和非球面特性(SF约为0.27)。

Material and methods: 18 cases of keratoconus and 7 cases of normal cornea was stained by haematine-eosin, iron stain, alcian blue stain method and observed by transmit electron microscope and scanning electron microscope.

材料与方法:取角膜移植术钻下的圆锥角膜组织18例,无眼病的尸体角膜7例,经苏木精-伊红染色、铁染色、甲苯氨蓝染色、阿尔新蓝染色和透射电镜和扫描电镜观察。

Objective To study the mechanism of haze formation and to investigate the expression of platelet-derived growth factor mRNA in corneal epithelium and stroma after photorefractive keratectomy.

目的 研究角膜上皮下混浊形成的发生机制,检测准分子激光屈光性角膜切削术后角膜上皮和基质血小板源性生长因子表达的变化。

Methods: 21 buttons of keratoconus, 7 buttons of normal cornea were detected.

材料与方法:取角膜移植术钻下的圆锥角膜组织21例,无眼病的尸体角膜7例,全部标本为石蜡包埋,免疫组织化学采用SABC法。

Objective: To observe the expression of βig-h3 in normal cornea and keratoconus, and discuss the effects of extracellular matrix on keratoconus.

目的:观察圆锥角膜患者及正常人角膜组织中βig-h3的表达,探讨细胞外基质结构成分在圆锥角膜病变中的作用。

Objective To explore the application of lamellar keratoplasty in ophthalmic emergency treatment.

目的 观察不同原因所致角膜缺损行板层角膜移植的临床效果,探讨板层角膜移植在眼科急诊中的应用。

TRLK is a new style of keratoplasty which possess both the therapeutic and refractive function it ...

治疗与屈光性板层角膜成形术是一种兼具治疗与屈光矫正功能的新型角膜成形术,可作为高度近视患者角膜溃疡的一种矫治对策。

Objective To determine the relationship between corneal thickness changes measured by ultrasonic pachymeter and outcomes after penetrating keratoplasty.

目的 为准确动态观察穿透性角膜移植术后患者角膜厚度的改变,了解角膜厚度改变与移植结果之间的关系。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果:(1)层粘连蛋白组处于G_2~M期细胞比例较对照组显著提高,Go~G_1期细胞比例显著下降,提示层粘连蛋白促进内皮细胞DNA合成,及细胞分裂增殖;光镜下,对照组细胞分布成团状,细胞密度较低,细胞间连接紧密,细胞内微丝结成网状,细胞核大小一致;与对照组相比,层粘连蛋白组细胞生长旺盛,细胞密度高,向周边伸出大量板状及丝状伪足,细胞内微丝增多、拉长、集结成束,伸入伪足中,细胞核形状大小不一致;(2)倒置显微镜观察及细胞生长曲线显示,各组细胞数目随时间增加而明显增多,各实验组较对照组增生显著,EGF和LN联合应用组各时间点细胞数目最高;实验组处于S期和G_2~M期细胞数目增加,Go~G_1期细胞数目减少;提示EGF、LN单独及联合应用均可促进细胞增殖,但尚不能认为二者有交互作用;(3)倒置显微镜下,组织培养的猫角膜内皮细胞排列成密集的单层,细胞间连接紧密;组织学观察发现,培养的猫角膜内皮细胞形成完整的内皮层,贴附于脱水基质的后弹力膜上,与正常的角膜内皮组织结构相似;扫描电镜下,组织培养的猫角膜内皮细胞间紧密镶嵌排列,可见某些细胞呈近似六边形排列,细胞大小不甚一致,胞核清晰。

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