角膜的

To investigate the change of corneal thickness after photorefractive keratectomy and the relationship between the corneal thickness and Haze.

目的：探讨准分子激光角膜切削术后角膜厚度变化，及角膜厚度变化与角膜雾状混浊的关系。

Objective To observe the effectiveness of penetrating keratoprosthesis implantation in patients who failed to or were not suitable for keratoplasty.

目的 应用穿透人工角膜植入术使角膜移植失败或难以进行角膜移植的角膜白斑患者脱盲。

Methods Five patients with leucoma induced by chemical burn and blast received Yakimenko keratoprothesis implantation.

应用Yakimenko型人工角膜对4例化学烧伤后角膜白斑和1例爆炸伤后的角膜白斑进行了穿透人工角膜植入术。

Twenty-six eyes which appeared rejection after allograft penetrating keratoplasty between April 2003 and April 2004 in Shandong Shi'erming Eye Hospital were collected, including 16 males and 10 females, 9 cases were repeated relapsing viral keratitis, 8 cases were traumatic posterior corneal leucoma, 5 cases were corneal decompensation caused by various intraocular surgery, 2 cases were congenital leucoma, and 2 cases had other symptom. The rejection occurred at 2 month to 2.5 years after operation. Among them 19 cases were transferred to our hospital and asked adding traditional Chinese therapy because western medicine was ineffective in other hospital.

选择山东施尔明眼科医院2003-04/2008-04接诊的同种异体穿透性角膜移植后发生排斥反应病例26例，男16例，女10例，反复发作病毒性角膜炎9例，创伤性后角膜白斑8例，各种内眼手术引起角膜失代偿5例，先天性角膜白斑2例，其他2例，上皮型免疫排斥反应、基质型免疫排斥反应、内皮型免疫排斥反应均有，排斥反应发生在术后2个月～2.5年，其中19例是外院西药治疗无效而转入山东施尔明眼科医院要求加用中医治疗。

Methods 500 normal cases (918 eyes), 200 long term soft contact lens wear cases (386 eyes) and 100 long term RGP wear cases (195 eyes) were enrolled in this study.

应用超声角膜测厚仪检测无角膜接触镜配戴史者500例（918眼）、长期持续配戴软性角膜接触镜2年以上200例（386眼）和长期配戴RGP≥1年者100例（195眼）的中央角膜厚度。

Our investigation demonstrates that before refractive surgery the keratometer has a similar clinical value as that of corneal topography, and the △ Sim K value is related to the graphic pattern of corneal topography.

角膜曲率计与角膜地形图对术前角膜散光测量具有相似的临床价值，角膜地形图图形与参数△Sim K值有关。

Methods: A total of 366 eyes with keratoconus were subjected to wear proper RGPCL. Keratometer and corneal topography were used to determine the central cornea astigmatism, apex K value, apex decenteation distance, SRI and SAI before, 12 and 18 months after wearing RGPCL.

对圆锥角膜患者选配合适的高透氧RGPCL，分别于配戴前及配戴后12、18个月行角膜曲率计和角膜地形图检查，采集角膜中央散光值、锥顶K值、锥顶偏心距离、SRI、SAI等参数，进行统计学分析。

Tissue engineering cornea stromata were constructed in vitro.

用去垢剂联合酶的方法脱细胞处理猪角膜基质，在去细胞猪角膜基质材料上接种体外培养的兔角膜基质细胞，并将兔角膜基质细胞进行PKH26荧光标记，在体外构建组织工程角膜基质。

Methods Chromosome, HE stainings and autoradiography were made for the cornea of human and monkey.

目的 探讨灵长目动物角膜内皮细胞的再生能力及方式方法对猴及人角膜做染色体染色、HE染色及放射自显影结果成年创伤活体猴角膜内皮细胞有再生能力成年及幼年灵长目动物角膜内皮细胞在生理和病理条件下的再生方式均为无丝分裂结论灵长目动物角膜内皮细胞在一定条件下有再生能力，其再生方式为无丝分裂

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。