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Primary culture of bovine corneal endothelial cells and whole rabbit corneas were used as the experimental materials. We first treated the cultured bovine corneal endothelial cells with PTPs inhibitor, sodium orthovanadate, with a variety of concentrations (25,50,100μM) for various durations (8,24 hrs). The effects of PTP inhibition on cellular distribution of cell-cell junctional proteins, such as N-cadherin, alpha-catenin and p120, were evaluated by immunohistochemical staining with fluorescein microscopy and confocal microscopy. Immunohistochemical staining with Ki67 Ab, a marker of cell proliferation, was also used to detect cells entering cell cycle.

实验材料为初代培养之牛角膜内皮细胞和全厚度之兔子眼角膜,使用sodium orthovanadate作为酪胺酸磷酸脢的抑制剂,接著以不同SOV浓度(25, 50, 100μM)及不同时间(8, 24 hrs)来处理初代培养之细胞与新鲜的兔子眼角膜,之后进行萤光染色并使用萤光显微镜及共轭焦显微镜进行影像撷取,以观察位於细胞与细胞间的蛋白质,例如:N-cadherin, alpha-catenin 和p120的改变,另外也使用Ki67抗体来侦测是否有进入cell cycle的细胞。

A pachometer is for measurement of corneal thickness.

测厚计是用于测量角膜厚度的。

Normal Chinese central corneal thickness was measured with Vida 55 pachometer.

结果显示:男性中心角膜厚度平均为0.516±0.027mm,女性为0.507±0.026mm。

Compared with other anterior segment tomographic devices like UBM, corneal pachymeter, OrbscanⅡ, IOL master, gonioscopy, etc., the Visante OCT has advantages of noncontacting, high resolution and reproducibility, obtaining image quickly, etc. At present, it can be used in the biological measurement of cornea, anterior chamber, iris and the central portion of the lens and ophthalmopathy research.

与目前临床应用的其他眼前节检测手段如超声生物纤维镜、超声角膜测厚仪、多功能眼前节分析诊断系统,光学生物测量仪、房角镜等相比,OCT具有非接触性、高分辨率、可重复性高、获取图像快等特点。

Both dosages of DIZ (2 mg/kg) and MID (2 mg/kg) with an increased KET dosage (from 60 to 90 mg/kg) shortened the time of diminished corneal reflex (from 5.3 ± 0.8 to 2.2 ± 0.3 min) and palpebral reflex (from 3.0 ± 0.4 to 1.3 ± 0.2 min) and significantly prolonged the duration of anesthesia (from 97.2 ± 18.0 to 187.8 ± 28.8 min vs. from 103.3 ± 9.4 to 181.8 ± 19.5 min), but did not reduce the recovery time.

於定量DIZ(2 mg/kg)和MID(2 mg/kg),KET由60 mg/kg增加至90 mg/kg,可分别缩短导入期角膜反射减弱(由5.3 ± 0.8分钟减至2.2 ± 0.3分钟)与眼睑反射减弱(由3.0 ± 0.4分钟减至1.3 ± 0.2分钟)时间,且能显著延长麻醉时间(分别由97.2 ± 18.0分钟增至187.8 ± 28.8分钟和由103.3 ± 9.4分钟增至181.8 ± 19.5分钟),但并未延长回复期。

Pannus is a form of superficial keratitis, or inflammation of the cornea, found most commonly in German Shepherd Dogs, Greyhounds, and Siberian Huskies.

角膜血管翳是一种浅表性角膜炎,常见于德国牧羊犬、意大利灵缇犬和哈士奇。

In Stevens Johnson syndrome the mucosal shedding in the tracheobronchial tree may lead to respiratory failure and ocular sequelae which may include anterior uveitis and corneal ulceration, with blindness that may develop secondary to severe keratitis or panophthalmitis in 3 to 10 percent of patients.

在史蒂文斯强森症候群粘膜脱落,在气管,支气管树可能会导致呼吸衰竭和眼的后遗症,其中可能包括前葡萄膜炎及角膜溃疡,失明的可能发展中严重的角膜炎或panophthalmitis在3至10 %的病人。

Major causes of limited visual acuity were irregular astigmatism resulting from central or paracentral corneal scar or developing macular pucker.

术后视力的改善受限于因角膜中央或旁中央疤痕所致的不规则散光及发展的黄斑皱褶。

Toxicity Ⅲ grade "+++" cell shrinked,pericellular widen,contrast microscopy×250

角膜上皮细胞&++&毒性反应,胞浆和胞核出现颗粒,倒置显微镜×250。

Toxicity Ⅲ grade "+++" cell shrinked,pericellular widen,contrast microscopy×250

角膜上皮细胞&+++&毒性反应,可见细胞皱缩、间隙加宽,倒置显微镜×250。

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