角膜

To study the mechanism of the immune tolerance of the xenogenic epikeratophakia,4 patients with keratoconus and 5 patients with aphakia were treated by epikeratophakia monkey lenticules.

摘 要 目的：研究异种表面角膜镜片术后机体对异种角膜的免疫反应，阐明人体对异种角膜的免疫耐受机制。

Objective: To explore the relationship between corneal storage methods and rejection after corneal transplantation, and to study the roles of intercellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor-α in aqueous humor and serum in keratoplasty rejection in rats.

目的：探讨角膜保存时间与术后免疫排斥反应的关系，检测角膜植片的细胞间黏附因子-1（ICAM-1）表达及房水、血清中肿瘤坏死因子-α含量与角膜移植排斥反应的关系。

This paper reported a new method using tangential curvature to establish a mathematical model of the anterior cornea and an analysis of the regularity of the distribution of the asphericity Q. The curvature in the horizontal meridians is a prolate ellipse. The curvature in the vertical meridians is an oblate ellipse. The curvature of the horizontal meridians is important for anterior corneal asphericity.

本研究分析了运用正切曲率半径值建立人眼前表面角膜数学模型的科学性，显示角膜前表面水平子午线方向非球面性趋向于长椭圆，垂直子午线方向非球面性趋向于扁椭圆，说明人眼角膜的非球面性特性主要由水平子午线实现。

From the results of ultimate stress, breaking energy and relaxation, there were no significant differences between transverse and longitudinal direction.

同时在考虑角膜外形和焦距对视网膜的作用时，特别是经过放射状角膜切开术后，角膜的力学特性将是重要的研究课题。

The animal models with limbal stem cell dysfunction were made by limbus region resection combined with curettement of the corneal epithelium.

以新西兰白兔为实验对象，采用角膜缘切除联合角膜上皮刮除的方法制作兔角膜缘干细胞缺乏的模型。

The animal experiment stage: 40 rabbits underwent corneal epithelium curettement and limbal ablation to induce limbal stem cell deficiency and were randomly divided into four groups, simple amniotic membrane group, non-frozen cells group, glycerol group and dimethyl sulfoxide group(n=10 for each group). Searching for the activity of frozen and non-frozen cells more completely by transplanting subculturing cells and observing the accomplishment of cells function.

动物实验阶段：将40只角膜缘干细胞损伤的模型兔随机分成单纯羊膜组、未冷冻细胞组、DMSO保存细胞组和甘油保存细胞组，每组10只，用传代培养2周后的角膜缘上皮细胞作移植实验，观察细胞体外功能完成情况来进一步比较深低温保存前后角膜缘上皮细胞的活性。

METHODS: The central corneal thickness, intraocular pressure, dioptre and corneal curvature were measured in 137 han nationality cases(274 eyes) and 115 Uygur myopia patients(230 eyes).

目的：分析维吾尔族与汉族近视患者中央角膜厚度(central corneal thickness，CCT)是否存在差异，了解维吾尔族近视患者中央角膜厚度与角膜曲率、眼压值及屈光度关系。

The differences of CCT between the Uygur myopia patients and the han nationality myopia patients and the relationship between corneal curvature, intraocular pressure value, dioptre and CCT in the Uygur nationality were analyzed.

对屈光中心就诊近视患者汉族137例274眼及维吾尔族患者115例230眼测量其角膜中央厚度、眼压、屈光度及角膜曲率，分析维吾尔族与汉族CCT差异性及维吾尔族CCT与角膜曲率、眼压值及屈光度关系。结果：维吾尔族CCT(514.94±31.94μm)与汉族CCT(514.86±29.50μm)无显著性差异(t=0.029，P=0.977)。

Methods Using the corneal topography analysis system, changes in topographic parameters and best corrected visual acuity were analyzed before surgery and 3 months after the surgical removal of pterygia in 56 eyes.

对56例(56眼)翼状胬肉行手术切除，分别于术前、术后使用角膜地形图仪进行检测，记录术前、术后第3个月的角膜地形图主要参数、最佳矫正视力，术前和术后以角膜地形图及综合验光仪验光法确定角膜散光情况，并进行统计学分析。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。