角膜

The donor cornea is measured and cut to fit a circular opening, which is made in the central part of the patient's cornea; the patient's cornea is lifted bloodlessly off -- there are no blood vessels in the cornea -- and the new cornea is sewn in with thread that is finer than a human hair.

捐助眼角膜是衡量和减少以适应开放的通知，这是在中央部分患者的角膜；患者的角膜bloodlessly取消了-没有血管的角膜-和新的角膜缝在与线程是细比人的头发。

However, epikeratophakia, deep lamellar keratoplasty, intrastromal corneal ring, corneal crosslinking, and penetrating keratoplasty are the surgical way for the keratocous that contact lens could not correct.

角膜表层镜片术、深板层角膜移植术、角膜基质环植入术、角膜交联疗法及穿透性角膜移植术是临床上常用的手术治疗方法。

Methods Keratometric reading with Topcon KR-8100 automatic keratometry and comeal topography with the TMS-4 on 53 ametropia' children, 106 eyes were compared. Difference in measurements of steep meridian power magnitude Ks, flat meridian power magnitude Kf, astigmatism magnitude Ks-Kf and location as well between the two methods were assessed using paired t test.Bland-Altmann method was used to evaluate the agreement of the two methods.

采用KR-8100自动角膜曲率计及TMS-4角膜地形图仪对53例（106只眼）屈光不正儿童进行测量，应用配对t检验对两种方法测量的陡峭子午线角膜屈光力、平坦子午线角膜屈光力、角膜散光Ks-Kf大小及轴向进行比较，并应用Bland-Altmann分析对两种测量方法进行一致性评价。

30 eyes of superficial corneal diseases including corneal dystrophy 12 eyes,scar after infection 7 eyes, dimmer nummular keratitis 6 eyes,scar after trauma 3 eyes and band degeneration 2 eyes were treated with PTK, and examined the uncorrected visual acuity,best corrected visual acuity and refraction respectively at one week,one month,three months and six months after PTK.The analysis of variance and chi-square test were used.

对角膜浅层病变30眼，包括角膜营养不良12眼，感染后角膜瘢痕7眼，钱币状角膜炎6眼，外伤性角膜瘢痕3眼和角膜带状变性2眼行PTK治疗，于术后1周、1、3、6月分别检查裸眼视力、最佳矫正视力和屈光状态等并回顾分析其疗效。

In the generalization of contact lens develops day by day, on the issue associated with corneal infection due to incorrect habit of contact lens is put much emphasis. Pseudomonas aeruginosa is one of the most important infectious pathogens, it will adhere on the cornea and destroy the corneal tissue opportunistically with its quite violent virulence. The infected corneas will be attacked to necrosis by one to two days with very fast destructive speed. It is very important to diagnosis the infectious pathogen in time.

在隐形眼镜愈来愈普及的趋势下，因隐形眼镜配戴习惯不正确造成角膜感染的议题也逐渐受到重视，而绿脓杆菌正是其中最重要的一种感染病菌，其会附著在角膜上，然后伺机地破坏角膜组织，绿脓杆菌毒性相当猛烈，故其破坏的效率相当地快，角膜大概一至二天就会被摧残溃烂，因此即时诊断出角膜感染的病原体是相当重要的。

The animal model of rabbit bullous keratopathy was established by anterior chamber injection with 0.05% benzalkonium bromide. The corneal thickness and corneal endothelial cell density were measured with ultrasonic corneal pachymeter and non-contact specular microscope separately before the animal models was established.

二、以0.05%的新洁尔灭溶液前房注射制作兔眼大泡性角膜病变的动物模型，制模前分别用角膜测厚仪和角膜内皮显微镜测量角膜厚度和角膜内皮细胞数目。

This article summarized the influence factor of ametropia after PKP and the main treating methods, including wearing on the glasses or adhesive glasses, taking out stitches of cornea selectly, loose the cut, etc.

对影响PKP术后屈光状态的因素及其主要矫治方法如配戴框架眼镜或角膜接触镜、选择性角膜拆线、松弛性切口、散光性角膜切削术、角膜楔形切除术、放射状角膜切开术、准分子激光手术等进行简要综述。

Tissue engineering cornea stromata were constructed in vitro.

用去垢剂联合酶的方法脱细胞处理猪角膜基质，在去细胞猪角膜基质材料上接种体外培养的兔角膜基质细胞，并将兔角膜基质细胞进行PKH26荧光标记，在体外构建组织工程角膜基质。

Methods Chromosome, HE stainings and autoradiography were made for the cornea of human and monkey.

目的 探讨灵长目动物角膜内皮细胞的再生能力及方式方法对猴及人角膜做染色体染色、HE染色及放射自显影结果成年创伤活体猴角膜内皮细胞有再生能力成年及幼年灵长目动物角膜内皮细胞在生理和病理条件下的再生方式均为无丝分裂结论灵长目动物角膜内皮细胞在一定条件下有再生能力，其再生方式为无丝分裂

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新