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The reverse transcription-polymerase chain reaction was used to seek the expressions of a specific fibroblast marker, prolyl 4-hydroxylase β, and a specific marker of epithelial cells, cytokeratin-4, in the cells.

采用逆转录多聚酶链反应检测hEFs表达成纤维细胞特异性标志(脯氨酰4-羟化酶β亚单位)和上皮细胞特异性标志(细胞角蛋白4)的情况,对该细胞进行鉴定。

Results The hEFs were successfully isolated and cultivated from gonadal ridges and dorsal mesenteries of human embryos. They could be passaged beyond the 25th generation. The biologic characteristics of the cells did not change, even in high-passage cells or frozen-thawed cells. The cells expressed prolyl 4-hydroxylase β, but not cytokeratin-4, which was similar to the fibroblasts. The cultured cells expressed bFGF and LIF.

结果:从人胚胎生殖腺嵴和肠背系膜中成功地分离培养出hEFs,该细胞可传25代以上,且经过传代及冻存复苏后生物学特性无改变;hEFs表达脯氨酰4-羟化酶β亚单位,不表达细胞角蛋白4,确定其为成纤维细胞;该成纤维细胞表达bFGF和LIF。

Where α is the rotation angle read on the polarimeter, C is the concentration of the semicarbazone solution in grams per milliliter of solution, and l is the path length of the polarimeter tube in decimeters (these units are employed for historical, rather than rational reasons).

是在旋光仪中的旋转角的读数,C是缩胺基脲溶液的浓度,以每千分之一公升溶液中的公克数来表示,而l是旋光仪试管的通路长度,以分米表示(这些单位都是历史沿用下来的,而没有合理的原因)。

NodD binds to and bends target promoters through anchoring two tandem and individual specific DNA sites. NodD functions as a tetramer, which has a V-shaped main body. Tetrameric NodD is to change its own conformation rather than its oligomeric forms in response to small signal molecules. The specific interaction between each NodD DNA-binding domain and each specific DNA site does not alter itself in spite of naringenin induction, and the induced conformational change is transferred from protein to DNA. Only the DNA conformation incited by induced NodD is competent for RNA polymerase to form the transcriptional open complex. It cannot be excluded that NodD may have protein-to-protein contacts with RNA polymerase, and that the NodD conformational change may also directly contribute to the transcriptional open complex formation. However, the NodD conformational change itself cannot serve as the determinant of the transcriptional molecular switch.

通过研究,我们提出了初步的NodD操纵子激活模型:第一,四聚体是NodD蛋白的功能单位,它通过铆钉两个串联的相对独立的DNA靶位点结合被诱导的启动子;第二,小分子配基的结合是改变NodD四聚体的构象而不是引发不同形式的寡聚体,在我们的模型中,NodD四聚体缩小其V形主体的弯折角,进而缩短其DNA结合功能域的间距;第三,小分子信号的诱导并没有改变NodD的DNA结合域和其DNA靶位点的相互作用,NodD的构象改变由蛋白质经其双铆钉位点传递给DNA;第四,只有诱导状态的NodD激发的DNA构象才能有效地使RNA聚合酶形成转录开放复合物;第五,不排除NodD与RNA聚合酶可能有直接的相互接触位点,不排除NodD构象的改变可能直接有利于RNA聚合酶形成转录开放复合物,但是我们认为NodD构象改变本身不是充当转录激活开关的决定因素。

Hexagon These are the base units , you can modify this class directly or use inheritaance to create your game hexes.

六角这些是基本单位,您可以修改这个类直接或使用继承来创建你的游戏hexes。

Under high magnification of 5653x, this scanning electron micrograph depicts the surface of an unidentified insect's compound eye, revealing photoreceptor cells, support cells and pigment cells that make up the repeating hexagonally-shaped units of a compund eye known as "ommatidia".

在高放大倍率的5653x ,这一扫描电镜的表面描述一名身份不明昆虫的复眼,揭示感光细胞,支持细胞和色素细胞构成重复六角型单位的化合物眼睛被称为& ommatidia &。

Shandong Liangshan County Animal Husbandry Bureau of breeding stock to promote station QQ513033622 units directly affiliated to Shandong Province Animal Husbandry Bureau, the Ministry of Agriculture to determine commodity good beef base,"leading aquaculture demonstration enterprises of Shandong Province", set of breeding, to allocate to promote large-scale animal husbandry in one group, a large number of the supply of purebred Simmental, Charolais cattle, Limousin cattle, Luxi cattle, Holstein cows, Holstein cows, improved cattle, purebred Small Tail Han sheep, Boer goats and Merino sheep, dairy goat , Poll Dorset sheep, Dorper sheep, Suffolk Sheep, Charolais sheep, fattening young bull, meat calves, Jia Ziniu, breeding cattle, to help the child cattle, and other fine varieties.

山东梁山县畜牧局种畜推广站QQ513033622山东省畜牧局直属单位,是农业部确定的商品良牛基地,&山东省养殖龙头示范企业&,集养殖、调拨推广于一体的大型牧业集团,大量供应纯种西门塔尔牛、夏洛莱牛、利木赞牛、鲁西黄牛、黑白花奶牛、荷斯坦奶牛、改良牛、纯种小尾寒羊、波尔山羊、美利奴羊、奶山羊、无角陶赛特羊、杜泊羊、萨福克肉羊、夏洛莱羊、育肥小公牛、肉牛犊、架子牛、育成牛、帮子牛、等优良品种。

Halothane and isoflurane (0, 0.4, 0.8, and 1.2 MAC) were tested in a counterbalanced crossover protocol.

在去大脑大鼠,从背角神经元终末到20个1HzC 纤维的电刺激,记录单个单位的数据。

To accomplish this, I m using aluminum angle rails and flat pieces which were purchased from the local Lowes Hardware store.

要做到这一点,我用的铝角导轨和单位件,其中购自当地lowes硬件商店。

Hormagaunts do extra damage when in big swarms and in synapse, and termagants make targets more vulnerable while shooting at them and can knockdown enemy units when in synapse range.

低级的泰伦单位也有些增强。角虫在大数量下和节点控制中造成额外伤害,节点控制中兵虫能使目标虚弱并且有击倒效果。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。