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观察的人

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METHODS: Eleven specimens of medulloblastoma were acutely dissociated and triturated into single cells without trypsin digestion.

本研究旨在利用简化的无血清培养基和悬浮培养方法,从人髓母细胞瘤中分离培养脑肿瘤干细胞,观察其在体外的增殖、分化。

My research tries to find the variational characteristics of the lung cancer patients'sublingual venae, which can be use as the basis for general investigations of highly dangerous people, and diagnose and treat lung cancer patients in the early stage. It will be play an important role in improving the level of peoples'health.

将研究对象分为4组,观察组选择经病理学检查或细胞学检查确诊的152例原发性支气管肺癌患者,其中男88例,女64例,平均年龄60.04±11.98岁,临床分期Ⅰ期32例,Ⅱ期26例,Ⅲ期52例,Ⅳ期42例,经病理确诊的共81人,其中非小细胞肺癌66例,小细胞肺癌15例。

The earth tilting on its axis is another re-occuring observation that we are hearing from Inuit, which they know because of how the sun, moon and stars have changed in the sky.

另一件事情是我们从因纽特人那里得知的,他们不断地观察到地轴在倾斜------太阳,月亮和星辰改变了在天空中的位置,他们从中了解到了这一情况。

Methods BHK cells were seeded into 96 plates to be cocultured with these solutions.

方法将BHK-21细胞分别种植于类人胶原蛋白、壳聚糖、透明质酸以及共混溶液中,在倒置显微镜下观察细胞的黏附和生长情况,并利用MTT方法检测种植后1~5 d细胞的增殖情况。

Methods PBMCs from 10 heparinized blood specimens of HIV-1 infected persons were collected to isolate the virus with PBMC cocultivation method. Neuramindase was added to the culture medium to raise the HIV isolation rate. The supernatant of cocultures were detected by p24 antigen capture assay and also IFA test was used to identify the isolates. Cell tropism of the HIV-1 isolates was tested byuse of H9 and MT4 cell lines, respectively.

采集10份福建艾滋病病毒感染者肝素抗凝血,分离外周血单核细胞,与健康人PBMC共培养进行HIV-1的分离,使用含神经氨酸酶的T细胞培养液提高病毒分离率,通过检测P24抗原、间接免疫荧光试验及电镜观察等确定病原分离结果。

Methods Human monocyte cell line THP-1 was cultured and induced to macrophage by PMA treatment. The effect of 9-cisRA on the differentiation induced by PMA was studied. Cell morphology was observed by phase contrast microscope and the adhesion rate of THP-1 was detected by methyl thiazolyltetrazolium assay. The cell surface markers involved in differentiation of mono cyte/macmphage (CD11b, CD36) were analyzed by flow cytometry.

体外培养人单核细胞系THP-1,经佛波酯诱导分化为巨噬细胞,同时给予9-顺式维甲酸对佛波酯诱导分化进行干预,通过相差显微镜观察细胞形态,MTT比色法检测细胞贴壁率,流式细胞仪检测与单核/巨噬细胞分化有关的细胞表面标志物CD11b和CD36的表达。

Up in the main room it's very simple, the evokers heal so make sure they are quickly killed off. Everyone that is in the room should stand in the middle but the Champions do a whirlwind attack so it's best to tank them a little bit out of the camp. Just AoE and watch your aggro and this event is also reasonably simple, the real fight starts when this part is over! There is one final note and this is an optional strategy. If you want to do the mind control strategy which will be mentioned later then you need to leave a Warbringer mob alive.

在主房间上方战斗那是相当简单,快速放倒召唤者治疗,所有人战到房间中央,T把黑暗符文保卫者拉到台子外面一点,使用aoe技能,并观察好仇恨,这是很容易办到的,这部分战斗结束,真正的战斗才开始,有一点注意,也可以用另一种方法:如果你要用心理控制,,那你必须让黑暗符文战争制造者活着。

We used the NIH3T3 fibroblast cells ascarrier because the cell clone is easy to expand,to betransfected and to express the exogenous gene.In the presentstudy,a model of fibro blast cell-mediated human IFN-〓 genetherapy was established and its anti-tumor activity was observed.

为此,我们选择了易于扩增、易于转染并能稳定表达外源目的基因的成纤维细胞为载体细胞,建立了人〓基因疗法的实验模型,并观察了其体内抗肿瘤作用。

METHODS: Under the same conditions, the samples of titanium alloy and CFR/PEEK were placed in the fresh human blood so as to determine blood compatibility via haemolysis rate, platelet activation level, and leukocyte activation level.

在相同条件下,将钛合金和碳纤维增强聚醚醚酮2种材质的试件分别放置于新鲜人血中,通过观察溶血率,血小板激活程度以及白细胞激活程度判断材料的血液相容性。

Objective To observed the effects of 5 hydroxyl group of genistein on recombinant human protein kinase CK2 holoenzyme activity.

目的观察染料木黄酮C5位上的羟基对其抑制重组人蛋白激酶CK2全酶活性的影响。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷

Nusa lembongan : Bali's sister island, coral and sand beaches, crystal clear water, surfing.

Nusa Dua :豪华度假村,冲浪和潜水,沙滩,水晶般晶莹剔透的水,网络冲浪。