观察的人

Banyan tree, many people have seen, but the careful observation of those who had banyan tree, but very few.

榕树，很多人都见过，但认真观察过榕树的人，却少之又少。

Berne observed that people need strokes, the units of interpersonal recognition, to survive and thrive.

伯尼观察到人需要安抚，安抚是社会性认可的单位，使人能够生存和成长。

Still,the porter of that institution is of an obese habit,and,according to the best of my observation of him,notvery impressible.

不过，博物馆看门人是个大胖子，而且据我认真观察，他还是一个不易被人打动的人。

Previous research and clinical observation had found that people with BDD tend to "focus primarily on details of their appearance at the expense of global or configural aspects," the researchers wrote.

有33人参与了这项研究，其中17人作为对照，另外16人患有BDD。以前的研究和临床观察发现，患有BDD的人容易&把主要精力集中在他们面容的细节上，而忽视了整体因素。&

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

He bid me observe it, and I should always find, that the Calamitles of Life were shared among the upper and lower Part of Mankind; but that the middle Station had the fewest Disasters, and was not exposd to so many Vicisitudes as the higher or lower Part of Mankind; nay, they were not subjected to so many Distempers and Uneasiness either of Body or Mind, as those were who, by vicious Living, Luxury and Extravagancies on one Hand, or by hard Labour, Want of Necessaries, and mean or insufficient Diet on the other Hand, bring Distempers upon themselves by the natural Consequences of their Way of Living; That the middle Station of Life was calculated for, all kind of Vertues and all kinds of Enjoyments; that Peace and Plenty were the Hand-maids of a middle Fortune; that Temperance, Moderation, Quietness, Health, Society, all agreeable Diversions, and all desirable Pleasures, were the Blessings attending the middle Station of Life; that this Way Men went silently and smoothly thro the World, and comfortably out of it, not embarassd with the Labours of the Hands or of the Head, not sold to the Life of Slavery for daily Bread, or harrast with perplexd Circumstances, which rob the Soul of Peace, and the Body of Rest; not enragd with the Passion of Envy, or secret burning Lust of Ambition for great things; but in easy Circumstances sliding gently thro the World, and sensibly tasting the Sweets of living, without the bitter, feeling that they are happy, and learning by every Days Experience to know it more sensibly.

他说，我自己可以从下面的事实中认识到，中间地位的生活确实幸福无比；这就是，人人羡慕这种地位，许多帝王都感叹其高贵的出身给他们带来的不幸后果，恨不得自己出生于贫贱与高贵之间的中间阶层。明智的人也证明，中间阶层的人能获得真正的幸福。《圣经》中的智者也曾祈祷：&使我既不贫穷，也不富裕。&他提醒我，只要用心观察，就会发现上层社会和下层社会的人都多灾多难，唯中间阶层灾祸最少。中间阶层的生活，不会像上层社会和下层社会的人那样盛衰荣辱，瞬息万变。而且，中间地位不会像阔佬那样因挥霍无度、腐化堕落而弄得身心俱病；也不会像穷人那样因终日操劳、缺吃少穿而搞得憔悴不堪。唯有中间地位的人可享尽人间的幸福和安乐。中等人常年过着安定富足的生活。适可而止，中庸克己，健康安宁，交友娱乐，以及生活中的种种乐趣，都是中等人的福份。这种生活方式，使人平静安乐，怡然自得地过完一辈子，不受劳心劳力之苦。他们既不必为每日生计劳作，或为窘境所迫，以至伤身烦神；也不会因妒火攻心，或利欲薰心而狂躁不安。中间阶层的人可以平静地度过一生，尽情地体味人生的甜美，没有任何艰难困苦；他们感到幸福，并随着时日的过去，越来越深刻地体会到这种幸福。

He bid me observe it, and I should always find, that the Calamitles of Life were shared among the upper and lower Part of Mankind; but that expos'd to so many Vicisitudes as the higher or lower Part of Mankind; nay, they were not subjected to so many Distempers and Uneasiness either of Body or Mind, as those were who, by vicious Living, Luxury and Extravagancies on one Hand, or by hard Labour, Want of Necessaries, and mean or insufficient Diet on the other Hand, bring Distempers upon themselves by the natural Consequences of their Way of Living; That the middle Station of Life was calculated for, all kind of Vertues and all kinds of Enjoyments; that Peace and Plenty were the Hand-maids of a middle Fortune; that Temperance, Moderation, Quietness, Health, Society, all agreeable Diversions, and all desirable Pleasures, were the Blessings attending the middle Station of Life; that this Way Men went silently and smoothly thro' the World, and comfortably out of it, not embarass'd with the Labours of the Hands or of the Head, not sold to the Life of Slavery for daily Bread, or harrast with perplex'd Circumstances, which rob the Soul of Peace, and the Body of Rest; not enrag'd with the Passion of Envy, or secret burning Lust of Ambition for great things; but in easy Circumstances sliding gently thro' the World, and sensibly tasting the Sweets of living, without the bitter, feeling that they are happy, and learning by every Day's Experience to know it more sensibly.

就我的社会地位而言，正好介于两者之间，即一般所说的中间地位。从他长期的经验判断，这是世界上最好的阶层，这种中间地位也最能使人幸福。他们既不必像下层大众从事艰苦的体力劳动而生活依旧无着；也不会像那些上层人物因骄奢淫逸、野心勃勃和相互倾轧而弄得心力交瘁。他说，我自己可以从下面的事实中认识到，中间地位的生活确实幸福无比；这就是，人人羡慕这种地位，许多帝王都感叹其高贵的出身给他们带来的不幸后果，恨不得自己出生于贫贱与高贵之间的中间阶层。明智的人也证明，中间阶层的人能获得真正的幸福。《圣经》中的智者也曾祈祷：&使我既不贫穷，也不富裕。&他提醒我，只要用心观察，就会发现上层社会和下层社会的人都多灾多难，唯中间阶层灾祸最少。中间阶层的生活，不会像上层社会和下层社会的人那样盛衰荣辱，瞬息万变。而且，中间地位不会像阔佬那样因挥霍无度、腐化堕落而弄得身心俱病；也不会像穷人那样因终日操劳、缺吃少穿而搞得憔悴不堪。唯有中间地位的人可享尽人间的幸福和安乐。中等人常年过着安定富足的生活。适可而止，中庸克己，健康安宁，交友娱乐，以及生活中的种种乐趣，都是中等人的福份。这种生活方式，使人平静安乐，怡然自得地过完一辈子，不受劳心劳力之苦。他们既不必为每日生计劳作，或为窘境所迫，以至伤身烦神；也不会因妒火攻心，或利欲薰心而狂躁不安。中间阶层的人可以平静地度过一生，尽情地体味人生的甜美，没有任何艰难困苦；他们感到幸福，并随着时日的过去，越来越深刻地体会到这种幸福。

He bid me observe it, and I should always find, that the Calamitles of Life were shared among the upper and lower Part of Mankind; but that the middle Station had the fewest Disasters, and was not expos'd to so many Vicisitudes as the higher or lower Part of Mankind; nay, they were not subjected to so many Distempers and Uneasiness either of Body or Mind, as those were who, by vicious Living, Luxury and Extravagancies on one Hand, or by hard Labour, Want of Necessaries, and mean or insufficient Diet on the other Hand, bring Distempers upon themselves by the natural Consequences of their Way of Living; That the middle Station of Life was calculated for, all kind of Vertues and all kinds of Enjoyments; that Peace and Plenty were the Hand-maids of a middle Fortune; that Temperance, Moderation, Quietness, Health, Society, all agreeable Diversions, and all desirable Pleasures, were the Blessings attending the middle Station of Life; that this Way Men went silently and smoothly thro' the World, and comfortably out of it, not embarass'd with the Labours of the Hands or of the Head, not sold to the Life of Slavery for daily Bread, or harrast with perplex'd Circumstances, which rob the Soul of Peace, and the Body of Rest; not enrag'd with the Passion of Envy, or secret burning Lust of Ambition for great things; but in easy Circumstances sliding gently thro' the World, and sensibly tasting the Sweets of living, without the bitter, feeling that they are happy, and learning by every Day's Experience to know it more sensibly.

他提醒我，只要用心观察，就会发现上层社会和下层社会的人都多灾多难，唯中间阶层灾祸最少。中间阶层的生活，不会像上层社会和下层社会的人那样盛衰荣辱，瞬息万变。而且，中间地位不会像阔佬那样因挥霍无度、腐化堕落而弄得身心俱病；也不会像穷人那样因终日操劳、缺吃少穿而搞得憔悴不堪。唯有中间地位的人可享尽人间的幸福和安乐。中等人常年过着安定富足的生活。适可而止，中庸克己，健康安宁，交友娱乐，以及生活中的种种乐趣，都是中等人的福份。这种生活方式，使人平静安乐，怡然自得地过完一辈子，不受劳心劳力之苦。他们既不必为每日生计劳作，或为窘境所迫，以至伤身烦神；也不会因妒火攻心，或利欲薰心而狂躁不安。中间阶层的人可以平静地度过一生，尽情地体味人生的甜美，没有任何艰难困苦；他们感到幸福，并随着时日的过去，越来越深刻地体会到这种幸福。

From the golden-tipped fields of mid-west America to the acient kingdoms of verdant Palestine,there is a happy truth to be shared with all who would take heed.

从美国的中西部金色的田野，到巴勒斯坦翠绿的古老疆土，那些留心观察的人共享着同一条恰当的真理。

Methods: cell morphology and flow cytometry were used to examine human mammary cancer cell line MCF-7 that treated with Ease powder aquous, and make use of the depressants of caspase-3 to apoptosis examination.

应用形态学观察和流式细胞术等方法对逍遥散提取液处理的人乳腺癌(MCF-7)细胞进行了检测和观察，并运用caspase-3抑制剂检测凋亡。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。