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Methods Myocardial ischemia was induced by subcutaneous injection of ISO (30 mg/kg for two days, once a day). The experimental animals were randomly divided into the normal group, ISO group, positive control group and three crocetin groups (25, 50, 100 mg/kg). Cardiac indexes were examined. The level of LDH, CK, MDA in serum were measured. The content of MDA, GSH-Px and the ATPase activity heart and mitochondria were assayed by colorimetric analysis. The histopathological change of myocardia was investigated by HE staining.

采用大剂量ISOsc诱导产生大鼠急性心肌缺血模型(1SO30mg/kg,每日一次,连续2d),实验大鼠随机分为正常组、ISO模型组、阳性对照组和3个西红花酸给药组(25,50,100mg/kg),测定心脏系数和血清中乳酸脱氢酶、磷酸肌酸激酶和丙二醛含量,观察心肌组织谷肮甘肤过氧化物酶、Na^ ,K^ —ATP酶和Ca^2 ,Mg^2 —ATP酶活性及心脏病理改变,观察西红花酸的保护作用。

After treatment with 50 mmol·L-1 crocin, the inhibited growth of tumor was observed. Electronic microscope was used to observe the morphological changes. The expressions of Bcl-2, Bax, Survivin and Cyclin D1 were detected by immunohistochemistry.

随机分为对照组和处理组。50 mmol·L-1 crocin处理后,观察其对肿瘤的抑制作用;电镜观察肿瘤组织形态改变;SP-免疫组织化学法分析Bcl-2,Bax,survivin,Cyclin D1蛋白表达。

MAIN OUTCOME MEASURES: Transfection rate and effect of ECV304 proliferation were calculated by fluorescence microscope and flow cytometer.

主要观察指标:①观察转染后对ECV304生长的影响,在流式细胞仪和荧光显微镜下计算转染效率。

MAIN OUTCOME MEASURES: Morphology changes of induced cells; introduced endothelioid cell surface antigen detected through immunocytochemical staining; the growth and adhesion condition of endothelioid cells on the intravascular stent observed under scanning electronic microsope.

主要观察指标:诱导后细胞形态变化,免疫细胞化学染色检测诱导后内皮样细胞表面相关抗原,扫描电镜观察内皮样细胞在血管支架上的生长黏附情况。

Methods Thirty patients (among 6~15 years old) met with DSM-I V for schizophrenia crit eria were enrolled as research group randomly and thirty healthy children and ad olescents marched age and sex were selected as normal control group.

随机选择年龄在6~15岁之间符合DSM-IV精神分裂症标准患者30例作为观察组,与观察组年龄、性别相匹配的健康者30例作为对照组。

To reflect, there must be the watcher and the watched, The experiencer heavy with the past.

要反射,就必定有观察者和被观察者、背负着沉重过去的体验者。

Group A as observing group were droped 0.02% nitroglycer in dose of 4 μg/kg into the nose 10min before extubation, and the SBP, MAP, DBP,HR were measured at 5min before extubation, extubate instant and 5min after extubation separately.

选择62例全麻患者,随机分为A、B两组,各31例。A组为观察组,于拔管前10min吸净双侧鼻腔分泌物及血液后,滴入0.02%硝酸甘油4μg/kg,观察拔管前5min、拔管即刻、拔管后5min的SBP、MAP、DBP、HR变化。B组为对照组,拔管前不滴硝酸甘油。

The filarial antibody levels were detected by using IFAT.

应用常规厚血膜法对残存微丝蚴血症进行追踪观察和定期对全民进行普查,在流行季节解剖致倦库蚊观察幼丝虫的感染率和感染度,以及应用 IFAT进行人群抗丝虫抗体水平测定。

Methods:Eightythree patients with galactophore cancer were stochastic divides into observation group and contrast group.

83 例乳腺癌患者,随机分成观察组和对照组。观察组43 例,接受以ADM为主的CAF方案(环磷酰胺+阿霉素+氟尿嘧啶)。

MAIN OUTCOME MEASURES: Gastrocnemius flexibility and incidence of lower extremity overuse injuries in the 2 groups.

主要观察指标:两组观察对象的腓肠肌柔韧性及下肢疲劳性损伤发生率。

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