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METHODS: Otocyst epithelia surgically isolated from fetal rats were cultured and passaged. Proliferation and differentiation of cultured cells were observed using BrdU labeling to find the progenitors. Immunocytochemistry (Factin, cytokeratin and calretinin) was used to detect cell properties of cultured cells.

取孕鼠胚胎听囊细胞进行体外培养,用免疫细胞化学方法,分别对培养的细胞进行细胞角蛋白、F肌动蛋白和calretinin染色观察细胞特征;BrdU染色观察细胞增殖。

METHODS摘要: Otocyst epithelia surgically isolated from fetal rats were cultured and passaged. Proliferation and differentiation of cultured cells were observed using BrdU labeling to find the progenitors. Immunocytochemistry (Factin, cytokeratin and calretinin) was used to detect cell properties of cultured cells.

方法摘要:取孕鼠胚胎听囊细胞进行体外培养,用免疫细胞化学方法,分别对培养的细胞进行细胞角蛋白、F肌动蛋白和calretinin染色观察细胞特征;BrdU染色观察细胞增殖。

Our results suggest that the oxhide pastern is the best way to observe stomatal movement of A.corniculatum compared with the puddle method and the nail poish pastern.

结果表明:搅碎法、指甲油印迹法、牛皮胶印迹法三种观察气孔方法中,牛皮胶印迹法是观察气孔开度变化的最佳方法。

The results suggested that the oxhide pastern print was the best way to observe stomatal movement of A. comiculatum compared with the blender method and the nail enamel print method.

结果表明:在搅碎法、指甲油印迹法、牛皮胶印迹法三种观察气孔方法中,牛皮胶印迹法是观察气孔开度变化的最佳方法。

Under transthoracic echocardiography, electrode catheters positioned intracardiac and target ablation were performed with the permentnent pacemaking catheter of left subclavian vein and the ablation catheter of right femoral vein.

在经胸心脏彩超引导下,采取"多切面观察"、"导管运动中观察",密切结合心内电生理定位,尝试左锁骨下静脉永久起搏导管和右股静脉消融导管的心内定位和靶点消融。

But is it possible to observe so that there is only observation, seeing, and nothing else - so that there is only perception without a perceiver?

但是否可能有这样一种观察,只有观察和看到,别的什么都没有——这样就有了没有觉察者的觉察?

The first two parts evaluated the changes of GMBF and pH of gastric perfusate after using these reagents.

前两部分分别观察用药后GMBF及胃灌流液pH值变化,第3部分观察各组动物用药后经0.15mol/LHCl-40%乙醇灌胃处理所致的胃粘膜溃疡面积。

The changes of mean percentage of TUNEL positive cells in dorsal root ganglion following bilateral sciatic ne...

观察神经挤压损伤后7、14、21和28d大鼠的背根神经节TUNEL标记阳性细胞百分比变化;利用蛋白印迹技术,观察神经再生相关蛋白(GAP-43)和神经生长因子受体表达水平变化。

METHODS Vein thrombus was formed in rat by ligating postcava. Thrombus formation in vitro was observed by Chandlers method. Clotting indexes were measure by ACL200 congulation system.

结扎大鼠下腔静脉观察对静脉血栓的形成,Chandler方法观察体外血栓形成,全自动凝血仪测定凝血指标和纤维蛋白原及ATⅢ、Ⅱ、Ⅱa活性。

Methods Reverse hemagglutination test was used for identification of Fral. The Y. pseudotuberculosis sensitive to PstI and the bacteriostastic ring were used for identification of PstI. The low-calcium medium was used for identification of Vwa and Jackson-Burrows medium was used for identification of Pgm.

采用反向间接血凝试验检测FraI;利用假结核菌对PstI敏感可产生抑菌环的复盖种子层法检测PstI;采用乏Ca(上标 2+)时37℃鼠疫菌不能生长观察Vwa,利用色素培养基观察Pgm。

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