观察孔

Methods Cells were maintained in DMEM medium with 10% fetal bovine serum. Five days before the beginning of experiments, the cells were seeded in phenol red-free DMEM medium containing 5% charcoal dextran-treated FBS. The cells were harvested, and seeded in 6-well culture plates or in 75ml flacks. After NP, BisA and DBP treatments for 72h, the cells were harvested, and mRNA and protein expression of PCNA, bcl-2 and bax were detected by reverse transcription-polymerase chain reaction and immunohistochemistry, respectively.

方法］T47D细胞在含10%胎牛血清的DMEM培养液中进行常规传代培养，实验前将细胞转移至无酚红DMEM（含5%活性碳葡聚糖苷处理过的FBS）中继续培养5d，收集细胞，PBS洗涤后接种於内置盖玻片的6孔板或75ml培养瓶中，用32×10^(-7)mol/L NP、32×10^(-7)mol/L BisA及32×10^(-6)mol/L DBP对细胞分别处理72h，用半定量RT-PCR技术观察这3种化合物对核增殖抗原PCNA、bcl-2及bax mRNA表达的影响，并用免疫组化方法对结果进行验证。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

The adsorption isotherms and local density profiles of methane in PZC were obtained.

在低温下，观察到了流体在层柱孔中的毛细冷凝现象以及吸附等温线上的迂回滞线环。

Methods Fifteen healthy, female SD rats were employed in the experiment. Sensitized systematically with keyhole limpet hemocyanin, the animals were inoculated with the same antigen through cochlea basal turn into the labyrinth. Adminstrated 5-bromo-2′-deoxyuridine intraperitoneally, the rats were sacrificed and the temporal bones were harvested at 3, 7,14 day after labyrinth vaccination respectively. The frozen sections of the decalcified samples were dealt with H-E staining and immunohistohemical methods to investigate the cellular infiltration, BrdUrd and IgG positive cells in the ES.

选用SD大鼠15只，以钥孔虫戚血蓝蛋白（keyhole limpet hemocyanin，KLH）全身免疫后，经耳蜗底回钻孔以相同抗原进行内耳免疫，然后分别在内耳免疫后3、7和14 d腹腔注射溴脱氧尿嘧啶核苷（bromodeoxyuridine，BrdUrd）后处死动物，取颞骨经组织学处理制作冰冻切片，用免疫组织化学技术观察内淋巴囊的细胞浸润、增殖和IgG细胞的分布状况。

The second link is "tracking account": In the last session, you took the opportunity to observe the ants Diao food back out of hole direction, so as to identify the number of indoor ant hole mouth.

第二个环节是&跟踪帐户&：在过去的会议上，你借此机会观察蚂蚁刁食品退出孔的方向，以确定室内蚁洞口数量。

OBJECTIVE: To observe the effect of the side-hole hollow bone cement nail on promoting bone fracture healing.

目的：观察股骨颈带侧孔空心钉添加骨水泥促进骨折愈合的效果。

For bulk cylinder-forming diblock copolymers, a series of novel structures such as helices and stacked toroids are identified which cannot form in bulk block copolymers.

对体相形成柱状相的双嵌段共聚物，在不同孔径的孔内我们观察到了一系列体相不存在的螺旋和层叠圆环等新奇结构。

The measured porosity data and the observations of thin sections and blue epoxy resin-impregnated thin sections show that the diagenesis in the Qingshui sag can be divided into two stages and five substages. There exist three abnormally high porosity zones vertically in the Cenozoic reservoirs in the Qingshui sag, Liaohe Depression, whose genesis is related to the eluviation of precipitation and the dissolution of organic acid.

根据岩心实测孔隙度、薄片观察、铸体薄片和扫描电镜等实际资料，将辽河盆地西部凹陷清水洼陷新生界成岩作用划分为早成岩阶段和中成岩阶段2个阶段，其中包括5个亚期，指出该洼陷新生界纵向上发育3个异常高孔带，这种异常高空带的形成与大气降水淋滤作用和有机酸对储层的溶蚀作用有关。

Objective: To investigate the effect of obturator nerve blockade guided by peripheral nerve stimulator combined with lower epidural blockade in patients with bladder tumor adjacent to the ureteral orifice.

目的：观察神经刺激仪引导定位闭孔神经阻滞复合低位硬膜外麻醉用于输尿管开口周围的膀胱肿瘤患者经尿道膀胱肿瘤电切术的麻醉效果。

On the contrary, CC with small vacuolar structures and few mitochondrias, endoplasmic reticulums were shown in weak light.

在弱光条件下也观察到叶绿体基粒片层边缘紊乱，筛孔处于阻塞状态。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。