观察仪

Titanium plate first was cleaned by glow Discharge using argon plasma to eliminate surface contaminants and to produce a consistent and reproducible titanium oxide surface layer. Then an intermediary allylamine deposition was covalently linked to the oxide layer by glow discharge, followed by the covalent binding of albumin to the free terminal NH2 groups using glutaraldehyde as a coupling agent. Surface morphologies were observed by scanning electron microscopy and atomic force microscopy.

样本以扫描式电子显微镜及原子力显微镜﹙AFM﹚观察表面形态变化，以能量分散光谱仪进行表面元素变化之定性分析，使用X光光电子能谱仪检测表面化学键结与元素变化作定性分析与半定量分析，并以胶样金免疫标示法配合使用场发射枪扫描式电子显微镜(Field Emission Scanning Electron Microscope， FEG SEM)，观察钛金属表面白蛋白连接情形作定性分析与定量分析。

The apoptosis rates of H22 ascitic cells were analyzed with flow cytometry 9 and 12 days after injection of H22 cells and the proliferation index was calculated.

观察各组小鼠的生存期；流式细胞仪测定腹腔注射后第9、12天各组小鼠腹水瘤细胞的凋亡率，计算增殖指数；腹腔注射后第12天电镜观察各组腹水瘤细胞涂片。

Methods MTT assay,electronic microscopy,flow cytometry and tumor volume assay were used to examine the effect of 23-hydroxyl betulinic acid on B16 melanoma cell line.

应用MTT分析，电镜观察，流式细胞仪，肿瘤体积大小测定等方法，对23-羟基桦木酸诱导黑色素瘤细胞进行检测和观察。

MAIN OUTCOME MEASURES: Transfection rate and effect of ECV304 proliferation were calculated by fluorescence microscope and flow cytometer.

主要观察指标：①观察转染后对ECV304生长的影响，在流式细胞仪和荧光显微镜下计算转染效率。

Objective:To observe the effect of butterflybush flower on the anti-proliferation of vascular endothelia cell.

目的 ：观察密蒙花对血管内皮细胞增生的抑制作用。方法：制备含中药密蒙花的大鼠血清，将其加入到培养的内皮细胞中，应用流式细胞仪观察细胞增生与凋亡的情况。

Trypan blue exclusion assay and cell growth curve were used to observe proliferation. Fluctuation of CD11b, reactive oxygen species and NF-кB were detected using flow cytometry. Cell apoptosis was observed using DNA laddering assay.

选择NB4细胞，加入ATRA和不同剂量的As2O3，观察细胞生长曲线，流式细胞仪检测CD11b、ROS、NF-кB的变化，DNA片段梯度观察细胞凋亡。

Methods The experimental mouse was injected with high, middle, and low dosages of marasmius androsaceus po1ysaccharide 5, 10, 20 mg kg^(-1d^(-1) in the abdomen, and the comparison group was given the same volume of saline water i. p. once a day. After treatment for 14 consecutive days, the blood cell count instrument was used to measure the mice hemogram, the phagocytic function of peritoneal macrophage and the ability of carbon granular clearance were observed., the ability of the spleen lymphocytes proliferation was tested by MTT, and the hemolysin production test was used to evaluate the influence of MA polysaccharide on specific humoral immunity in mice.

小鼠腹腔注射不同剂量的鬼毛针多糖5， 10， 20 mgkg^(-1d^(-1)，对照组给予等量生理盐水，每天给药1次，连续14d后，用血细胞计数仪检测小鼠血象；用腹腔巨噬细胞吞噬鸡红细胞实验、碳粒廓清实验观察小鼠吞噬细胞的吞噬能力；采用MTT法测定小鼠脾T淋巴细胞增殖能力；采用血清溶血素形成实验观察鬼毛针多糖对小鼠特异性体液免疫功能的影响。

Methods 30 patients with trichloroethylene medicamentous dermatitis and 30 normal persons were selected as the observation group and the control group respectively; color Doppler was used to detect the spleen thickness and longitudinal length, spleen vein and artery diameters, the maximum blood velocity and blood flow of spleen vein and artery; RIA was applied to determine the serum levels of HA, LN, PCⅢ, CⅣ and CG; relevant analysis was made on the linear relationship between serum indices of hepatic fibrosis and spleen hemodynamics of the two groups.

选择30例三氯乙烯药疹样皮炎患者作为观察组，30例同时期健康体检者作为对照组，用彩色多普勒超声仪，检测脾脏厚度及长径、脾动静脉直径、脾动静脉血流量和脾静脉最大流速SPV(下标 x。用放射免疫法检测肝纤维化指标透明质酸、层粘连蛋白、Ⅲ型前胶原、Ⅳ型胶原和甘胆酸的水平，并对观察组与对照组的脾血流动力学参数与肝纤维化指标进行相关分析。

C2C12 cells were induced to differentiation into muscle cells in vitro and the myogenesis model was detected by inverted microscope, RT-PCR, western-blot, cell immunofluorescence histochemistry, flow cytometry and confirmed to be successful. On the foundation of differentiation model, miR-206 was detected by northern hybridization and the result showed that the expression level increasing gradually during muscle differentiation with a character of phase specificity.

利用C2C12细胞在体外成功诱导其向成肌细胞分化，通过形态学观察，RT-PCR、western-blot、细胞免疫荧光组织化学及流式细胞仪等方法进行检测，证实我们所建立的细胞分化模型是成功的，并观察到随着诱导时间的延长，miR-206在成肌分化过程中表达量逐渐增高的，表达改变具有阶段特异性。

Methods: Rat MSCs were isolated with percoll and nonadherent cells were discarded 48 hours later. The shape was observed and CD34, CD44, CD45, CD166 were detected with Flow cytometry analysis. The growth curve of MSCs in different concentration of fetal bovine serum was observed by MTT colorimetry. The growth curve and attachment efficiency of MSCs of passage 1 (P1), P3, P5 and P10 were also observed.

采用密度梯度离心法结合贴壁分离法分离培养大鼠骨髓MSCs，观察其形态，流式细胞仪检测细胞表面标记，MTT法观察不同浓度的胎牛血清对MSCs生长曲线的影响及不同代MSCs的生长曲线和细胞贴壁率。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。