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Methods: Parallel-plate-flow-chamber was used to assay the influence of shear stress on endothelial cells morphology and cytoskeleton and apoptosis and NO secretion. endothelial cells morphology was observed under microscopy, cytoskeleton was showed by Coomassie brilliant blue staining, fluorescence stain, and transmission electron microscopy, apoptosis was detected by FCM, NO of circulating culture medium was assayed by NO kit.

细胞形态显微镜下直接观察;细胞骨架通过考马斯亮蓝染色、荧光标记和透射电镜进行观察;细胞凋亡通过流式细胞仪检测进行分析,一氧化氮通过流动循环培养基的收集后应用检测试剂盒进行检测分析。

MAIN OUTCOME MEASURES: At 1, 3, 5, 7, 14 days following immersion, microstructure of the sample surface was observed using scanning electron microscope. The structure of composite was analyzed utilizing X-ray diffractometer.

主要观察指标:于浸泡1,3,5,7,14 d后采用扫描电子显微镜观察样品表面形貌的显微结构,复合材料的结构采用X射线衍射仪进行表征。

In this beamline, with the neutron depolarization technique, provides information of the mean domain size, the mean square direction cosine of the magnetic induction in the domains, and the mean magnetization of a magnetic sample.

三维去极化中子仪主要为观察极化中子通过磁性材过程,受到内部磁区的扰动使得极化中子向的方向和大小改变,进分析后可以观察出材内部平均磁区大小、平均感应磁场强、磁区平均方向余弦的平方。

The American DIASONIC - FX double - function Ultrasound was used to observe file 2 - dimension sound-imagine-picture of file mass for the measurement of the blood flow with colored blood - flow image.

采用美国DIASONICS-GATEWAYfX双功能超声仪,先观察乳腺肿块二维声像图,再用彩色血流显像观察肿块内及周边血流情况,并给予量化。

The thermal properties, mechanical properties of PI-MWNTs nanocomposite were analyzed by Thermogravimetric analysis, Universal Test Machine and Hardness Pencil Test. The morphology of PI-MWNTs was observed by scanning electron microscopy. The dispersion of MWNTs in matrix were observed by transmission electron microscopy. The electrical property of composite were obtained by Super Megohmmeter.

研究中使用傅立叶转换红外光谱仪来鉴定其化学结构,热重损失分析仪探讨材料的热稳定性质,万能试验机及铅笔硬度计观察其机械性质,扫描式电子显微镜与穿透式电子显微镜来观察材料的表面型态及奈米碳管於基材中的分散性,超绝缘仪量测材料的电性。

METHODS Vein thrombus was formed in rat by ligating postcava. Thrombus formation in vitro was observed by Chandlers method. Clotting indexes were measure by ACL200 congulation system.

结扎大鼠下腔静脉观察对静脉血栓的形成,Chandler方法观察体外血栓形成,全自动凝血仪测定凝血指标和纤维蛋白原及ATⅢ、Ⅱ、Ⅱa活性。

Methods The effects of various concentrations of tea polyphenols on proliferation, invasion and apoptosis of CGTH W-3 cells were observed by MTF assay, plate scarification assay and flow cytometer, respectively.

方法用MTT法检测细胞增殖活性,观察不同浓度茶多酚对CGTH W-3细胞增殖活性的影响;并通过培养板划痕法观察茶多酚对CGTH W-3细胞侵袭能力的影响;应用流式细胞仪检测各浓度茶多酚诱导CGTH W-3细胞凋亡的作用。

Methods: To observe the effect of individual and associated drugs on HeLa cells the methyl thiazolyl tetrazolium assay was used.

采用MTT例定法观察顺铂、5-氟尿嘧啶及甲氨喋呤单用及合用对Hela细胞的影响,流式细胞仪观察凋亡及细胞周期的变化。

To produce medicine serum of Liu-Shen-Wan with different dose, different time of pouring medicine, different time of blood sampling.2. To measure content of arsenic in serum with atomic absorption method, to qualitative analysis toadfish in medicine serum of Liu-Shen-Wan with thin layer chromatography.3. MTT and trypan blue assay detects the growth inhibition effect of Liu-Shen-Wan on HL-60 cells and to make certain the best scheme of making method of medicine serum of Liu-Shen-Wan.4. Gimsa dying assay observes morphology changes of apoptosis .In situ end-labeling method and flow cell machine quantificationally probes apoptosis phenomena.

1 分不同的给药剂量、给药天数和不同的采血时间制备含药血清。2 用原子吸收法测定血清砷含量;薄层色谱法定性检测六神丸含药血清中蟾酥成分。3 用MTT法、胎盘蓝染色法观察六神丸对HL-60细胞的增殖抑制作用,确定最佳血清制备方案。4 用吉姆萨染色法观察细胞凋亡的形态学变化,流式细胞仪、原位末端标记法定量检测细胞凋亡程度。

This experiment counts based on the direct digital frequencysynthesis the basic principle, fully II and hardware LT - IV testedthe box using ALtera Corporation's FPGA chip FLEX10 in softwareMaxplus in the platform to complete the DDS system design, hasdesigned a frequency and the phase may control has the sine and thecosine output direct numeral frequency synthesizer, was allowedto control and to observe the profile through the downloading boardand the oscilloscope, completed in the enhancement request hascontrolled the frequency and the phase through the downloading boardon pressed key, as well as many kinds of profiles, includingsquare-wave, saw-tooth wave, triangle wave and cosine wave choiceoutput, In addition on digital tube dynamic demonstration productionprofile frequency, and produces the tbl document from the simulationprofile, simultaneously draws the profile using the matlab higherorder language.

涂层测厚仪-帮忙翻译一下,谢谢涂层测厚仪-帮忙翻译一下,谢谢帮忙翻译一下,谢谢本实验基于直接数字频率合成计的基本原理,充分利用ALtera公司的FPGA芯片FLEX10在软件MaxplusⅡ和硬件LT—IV实验箱的平台上完成了DDS系统的设计,设计了一个频率和相位均可控制的具有正弦和余弦输出的直接数字频率合成器,通过下载板和示波器可以控制和观察波形,在提高要求中完成了通过下载板上的按键来控制频率和相位,以及多种波形,包括方波、锯齿波、三角波和余弦波的选择输出,此外在数码管上动态显示生成的波形频率,并且从仿真波形中生成tbl文件,同时利用matlab高级语言画出波形。

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