观察仪

Methods: The specimens of peripheral blood were collected from 15 individuals and each specimen was divided into three parts, one processed in an hour and the other two stored at -80℃ and -20℃ respectively Two different reagents (TRI Reagent and TRI Rcagent-BD) were used to extract RNA directly from whole blood followed by reverse-transcription polymerase chain reaction for amplifying 3-Actins gene. The RT-PCR products were detected by the ultraviolet transilluminator after 1.5% agarose gels electrophoresis and being stained by ethidium bromide.

采集人体外周血标本15例，分成三份，分别作为新鲜血组、-80℃冻存血组和-20℃冻存血组标本，用TRI Reagent和TRI Reagent-BD两种不同的试剂提取RNA，以RT-PCR方法扩增β-Actin基因，经1.5%琼脂糖凝胶电泳，溴化乙锭染色，紫外透射仪下观察分析。

HL-60 cell line was treated with As2S3 at different concentrations and different period,and cell growth was analyzed with trypan blue exclusion.

以不同浓度的As2S3在不同的时间处理体外培养的HL-60细胞株，细胞生长测定、形态学观察及流式细胞仪检测等多种方法，在体外研究As2S3对HL-60细胞凋亡和增殖抑制的作用。

Immunofluorescence assay was used to detect endothelial cell uptake and absorption acetylated low density lipoprotein and ulex europaeus agglutinin-Ⅰ. EPCs quantitative detection of surface markers was performed by flow cytometry: CD133, CD34 antigen expression and KDR.

通过免疫荧光法观察内皮细胞吸收乙酰化低密度脂蛋白和荆豆凝血素Ⅰ情况，流式细胞仪定量检测血管内皮祖细胞表面CD133、CD34和KDR抗原表达阳性率来鉴定血管内皮祖细胞。

Electrophysiological index before and after injection was recorded by using electromyograph so the neural electrophysiological influence by the injection could be observed.

用丹迪CANTATETM型肌电图仪检测用药前每组动物的电生理指标及用药组每次给药后的电生理指标，来观察硬膜外腔给药是否会对实验动物的神经电生理产生影响；5。

Methods: During intravenous anesthesia, twenty male SD rats received single transcranical magnetic and electric stimulation with Mag 2 stimulator and Cantada electromyograph. The characteristics of MEP recorded epidurally or in the bilateral forelimbs′ extensor muscles and hindlimbs′ gastrocnemius muscles under different stimulation intensities were observed.

20只雄性SD大鼠经静脉麻醉后采用 Mag 2型磁刺激仪和Cantada型肌电图仪分别进行单次经颅磁及电刺激，观察不同刺激强度下，在T12硬膜外和双侧前肢伸肌和后肢腓肠肌记录的MEP变化特征。

The expression levels of Bcl-2 protein were assayed by Western-blot and cell proliferation was measured by MTT method.

通过倒置荧光显微镜和流式细胞仪观察细胞的转染效率，Western印迹法检测Bcl-2蛋白表达水平，MTT法测定细胞增殖情况。

Methods Hyperlipemia model was reproduced in mice, and then pericarpium zanthoxylum oil was garaged for 4 weeks. Blood samples were obtained from retro-ocular vessel.

取健康昆明种小鼠建立高脂血症模型，同时花椒仁油灌胃4周，摘除眼球取血，用试剂盒检测血脂值，用流变仪测定血液流变参数，光镜观察小鼠肝脏病理变化。

Phase identification and microstructure observation of the composites were performed by X-ray diffraction, Electron Probe Microanalyses and transmission electron microscopy. The results show that ZrB2 phase is equiaxed or near equiaxed, which is hexangular and less 1μm in size. ZrAl3 phase is long strip-shaped. The in-situ reinforcements are distributed uniformly in the matrix and the interface is clean,without any other chemical products.

借助于X射线衍射仪、电子探针和透射电镜对复合材料的物像和增强相进行了观察，结果表明ZrB2颗粒呈等轴或等轴颗粒状，为六方结构，尺寸大部分小于1μm，ZrAl3相大部分呈长条状，两种增强相整体分布较为均匀，且与Al的界面光滑洁净，没有其它界面生成物。

objective we start with clinical research to explore the ability and mechanism of ion conduction with chinese herbs to curing ovulatory obstacle.methods adopting the methods of clinical observation random contrast.select the cases of ovulatory obstacle dividing randomly into two groups:therapeutic group and control group,the patients of therapeutic group using ion conduction with chinese herbs with act'on nourishing blood and promoting blood circulation to dissipate blood stasis when follicle grow up to 1.4cm.we take the decoction of jia jian tao hong si wu tang conducting the unilateral lower abdomen which has the dominant follicle,treating continuously for 4～8 days and observing ovulation.the patients of control group inject hcg 10000unit when follicle grow up to 1.8cm and observing ovulation.appraising two groups ovulated rate and pregnancy rate,and detect fsh、lh、e2、p at sixteen to eighteen days of menstrual cycle.results ion conduction with chinese herbs can significantly inspires ovulation and its effection is evidently higher than control group and has a higher pregnancy rate,no side effect of ovarian hyperstimulation syndrome.conclusion ion conduction with chinese herbs has significant function on inspiring ovulation.the target effection of treating ovulatory obstacle is exact.this method has a ovulated rate and higher pregnancy rate.

采用临床观察、随机对照的方法，选择排卵障碍的患者，随机分为治疗组和对照组，治疗组在b超监测卵泡生长达到1.6cm时，给予具有养血、活血化瘀作用的加减桃红四物汤水煎剂，用离子导入仪将中药水煎剂导入有优势卵泡的一侧少腹部，连续治疗4～8时天，同时连续监测卵泡排出情况。对照组在b超监测卵泡达到1.8cm时，开始采用hcg10000u肌注，连续b超监测卵泡排出情况。评价两组排卵率及受孕率，同时在月经周期的第16～22天抽血查fsh、lh、e2、p。结果运用离子导入的方法将具有促排卵作用的加减桃红四物汤水煎剂导入有优势卵泡的一侧附件，其促排卵的作用优于用hcg肌注促排卵，而且受孕率高，无卵巢过激综合征之虞。结论中药离子导入具有显著的促排卵作用，其治疗排卵障碍的靶向作用确切，排卵率及受孕率高。

With XG-2 functional tester of blood circulation, tests before and after reflexing Qigong (24 cases) andChanmigong (28 cases) were carried out in healthy old-aged and middle-aged subjects.

但是在国内，对正常人因练气功引起的循环生理变化的研究很少「，特别是运用无创性血液循环功能侧试仪，对几种气功的综合观察，尚未见报道。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。