观察仪

Morphologic change was observed with photomicroscopy and fluorescent microscopy.

普通光学显微镜及荧光显微镜观察榄香烯哌嗪对HeLa细胞形态的影响，利用流式细胞仪检测HeLa细胞凋亡及细胞周期变化情况。

The shape and size distribution of ice crystals in the frozen wet gluten were studied indirectly by microscopy and mercury porosimeter.

用扫描电镜和压汞仪间接观察和分析了冷冻的湿面筋蛋白中冰晶的形状和粒度分布。

Transverse section al anatomy and clinical significance of the pterygopalatine space

方法选用成年尸体头颈部20例制成连续横断面，观察翼腭间隙及其结构的解剖学关系，利用游标卡尺及求积仪分别测量其长径、宽径和面积。

The cell retention and repopulation on these materials were observed in vitro.

在流变仪施予体外流场之后，可观察细胞在基材上之残存率与其后再长回之情形。

Get the neonate Sprague-Dawley rats medulla spinalis, through the conventional primary cell culture procedure, we use the method of difference adherence time and get the motor neurons of anterior spinal cord. When the neuron is in maturity, we make the mechanical injury model in vitro. All the models were divided into four groups: group A is control group; group B is 100μM ATP group; group C is 100μM ATP+20μg/ml suramin group and group D is 100μM ATP+10μM ouabain+10μg/ml Thapsigargin group. Culture the four groups neurons, after one day, we count the motor neuron, observe the survival and activity of neurons through MTT shade selection experiment, use flow cytometry to analyze the percentage of apoptosis of motor neurons of anterior spinal cord and detect the expression of protein p-GSK-3β(ser9) through Western-Blot technology.

方法取新生大鼠脊髓，通过常规的原代细胞培养程序，采用差速贴壁法分离出大鼠脊髓前角运动神经元，培养成熟后制作神经元机械损伤体外模型，分为A组：对照组、B组：100μMATP组、C组：100gMATP+20μg/ml Suramin组和D组：100μM ATP+IOμM Ouabain+10μg/ml Thapsigargin组，对各组机械损伤的运动神经元进行培养，1天后分别进行运动神经元计数、MTT比色实验观察运动神经元的存活及活性、流式细胞仪分析机械损伤的脊髓前角运动神经元凋亡百分率和Werstern Blot技术检测p-GSK-3β(ser9)的表达。

Study the bioactivity of the n-HA/PA66 composite and the effects it would be to body's metabolism of calcium and phosphorus ion in vivo.(3) Study the osteo-conductivity and the ability to repair bone defect of the porous n-HA/PA66 composite and the feasibility use it as the scaffold of bone tissue engineering. Objects and Methods as follows: 1.To evaluate the biocompatability of nano-hydroxyapatite crystals and polyamide composite (n-HA/PA66) with the L929 cells.To proceed the morphological observation and take pictures of L929 cells after 1d,2d,4d,and 7d of co-cultured with extract of n-HA/PA66 ,and direct contact with n-HA/PA66.To determine light absorbtion value of every hole under 500 nm with enzyme linked immunity instrument after 1 d,2 d,4 d,and 7 d of contact of n-HA/PA66 extract with L929 cells,and direct contact with n-HA/PA66.In the meanwhile calculate the relative multiplication rate of cells,and evaluate them by six degree tests for cytotoxicity. To investigate the acute and chronic toxic reaction on the whole body induced by the new nano-hydroapatite crystals and polyamide composite(n-HA/PA66)after implanting in vivo and its effects on partial constitution of animal organs after implanting in vivo,and evaluate the potential and degree of subcuticular stimulation reaction.

本实验主要由以下三部分组成：一、n-HA/PA66 复合材料在动物体内、体外的生物相容性及生物安全性评价二、n-HA/PA66 复合材料植入动物体内的生物活性及近期对机体钙、磷代谢影响的实验研究三、网孔 n-HA/PA66 复合材料作为支架修复兔桡骨节段缺损的动物实验研究主要研究目标及方法如下：参照 GB/T16886.5-1997-ISO 10993-5：1992《医疗器械生物学评价细胞毒性试验体外法》之评价标准和要求，采用规定的 L929 细胞(小鼠结缔组织成纤维细胞)，分别经直接接触和材料浸提液与细胞共培养等方式对 n-HA/PA66 复合材料进行细胞毒性测试，采用细胞形态观察法观察两种细胞各组在 24h、48h、72h、5 天后各时相点的细胞形态学变化，并在显微镜下照相，从而对细胞与材料的生物相容性进行定性评价；同时采用细胞生长抑制法，以酶标仪定量测定评价各组 1，2，4，7 天 L929 细胞的相对增殖率，以定量测定并判别材料对细胞的毒性程度。

Pristine Li-Al LDHs are synthesized by hydrothermal process in different reaction conditions by varying the aging time for 1 and 24 hours. Both the Li-Al layered double hydroxides (abbreviated as Li-Al LDH1 and Li-Al LDH24 for aging time 1 hour and 24 hours, respectively) were modified by using sulphanilic acid sodium salt hydrate, modified agent to form modified Li-Al LDH1-SAS and Li-Al LDH24-SAS. The morphology of the pristine layered double hydroxides are investigated by using wide angle X-rays diffraction, scanning electron microscopy and particle size analyzer which indicates that the crystallinity and aspect ratio is increased with increasing the aging time from 1 hour to 24 hours. The particle size of Li-Al LDH24 and Li-Al LDH1 are found to be ~1000 nm and ~250 nm, respectively. Both the pristine and modified LDHs are characterized by XRD, FTIR spectra and thermogravimetric analysis.

本研究目的在於改变长晶时间的长短，合成出同径大小的Li-Al LDHs，再经由改质剂sulphanilic acid sodium salt hydrate将Li-Al LDHs无机层材表面有机官能化制备出改质型Li-Al LDHs-SAS，於是进一步藉由扫描式电子显微镜、射径仪和穿透式电子显微镜等仪器分别观察LDHs改质前和改质后其主层结构型态上的变化；从中可以发现Li-Al LDHs随著长晶时间的增加其径有随之增大的趋势，但经有机化改质后其径会由於改质环境的影响明显低许多，针对此现象本实验将未改质和改质后之Li-Al人工无机层材制备成复材进一步探讨其在热性质和难燃特性上的为表现。

33 patients with AVD were studied by dynamic three-dimensional(3-D)reconstruction.18 men and 15 women 14－56 of age involved in this sutdy.Hewlett Packard 2500 echocardiography with 5.0/3.7 MHz multiplane transesophageal probe was used for data acquisition,and Tomtec echo scan system used for 3-D reconstruction.Acquired images were extracted and reconstructed by computer system.The condition of ATC and valve were viewed from atrioventricular lateral aspect.Results:The valvular replacement operations were performed on the patients suffered from thickened,calcified,thinned or broken tendinous cordae.

我们应用动态三维重建技术，研究了33例房室瓣病变患者的腱索，其中男性18例，女性15例，年龄14-56岁，全部病例经手术及/或心导管证实，图像采集使用HP SONOS 2500超声心动图仪(配有3.7/5.0MHz的多平面经食道探头)，三维重建采用TOMTEC ECHO SCAN系统，将采集的图像提取并重建，从房室的侧面观察房室瓣腱索及瓣膜情况。

he correlation between the interphasial interaction and microstructure in sixpolyblend systems containing thermotropic liquid crystalline polymers hasaam investigated by means of dynamic mechanical spectrometry and scanning electron microscopy.

用动态力学谱仪和扫描电子显微镜测试观察了六个含热致液晶聚合物共混体系中的相间相互作用与微结构。

The results indicated thatthe movement of the patellofemoral joint and tibiofemoral joint occur at two points.

本文采用1000毫安 X 线录像和一维测力仪，观察举重运动员膝关节运动时股四头肌和髌骨所承受的应力。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。