观察仪

The cell phenotype was observed at 6,12,24 and 48h and the cellular proliferation was examined by methyl thiazolyl tetrazolium method.

将幽门螺杆菌野生株与cagA基因缺失突变株与BGC823细胞共培养，分别在6，12，24和48h观察细胞形态学变化，采用四甲基偶氮噻唑蓝比色法检测细胞增殖活性，流式细胞仪检测细胞凋亡情况。

Methods The mouse thymocyte model of apoptosis was induced by dexamethasone. The apoptosis ratio was observed with flow cytometry technique.

方法采取体外细胞培养 Balb/c小鼠(体质量 20 g左右的 Balb/c小鼠，雌雄不限)胸腺细胞的方法，制备地塞米松诱导的细胞凋亡模型，通过流式细胞仪观察凋亡细胞数量的变化情况。

Methods 40 ICR mice were divided into four groups: the control,the low(0.5 mg /kg),the middle(4 mg/kg) and the high(20 mg/kg),intragastric through mouth for 21 d,detected the thymocyte apoptosis by flow cytometry,observed the fas protein expression in thymus by immunohistochemistry.

方法ICR种小鼠体重20~25 g，雄、雌各半，共40只，随机分为4组，每组10只，即1个对照组和低、中和高3个组，经口灌胃21 d，流式细胞仪检测胸腺细胞凋亡状况，免疫组化染色，观察Fas蛋白在胸腺的表达。

Methods: THP-1 cells were induced by oxLDL in vitro. The CD11b, CD54 expressions and adhesion to human umbilic al vein endothelial cells were measured after treatment with probucol at different concentrations by flow cytometry and β-nitrophenyl N-acetyl-β-D -glucosminide test.

分别应用流式细胞仪和β-N-乙酰氨基己糖苷酶比色法，观察不同浓度普罗布考对oxLDL诱导的THP-1对人脐静脉内皮细胞的粘附及粘附分子CD 11b和CD54表达水平的影响。

In 9 patients with acute lymphocytic leukemia, all leukocyte histogram showed small cell unimodal distribution and expanded from small to middle cell areas.

方法对52例骨髓检查确诊为白血病（急性白血病46例，慢性白血病6例）患者行静脉采血，用KX-21N全血细胞仪进行全血细胞计数，观察红细胞、白细胞和血小板各参数检测结果和白细胞直方图分布变化。

Methods : To study the therapeutic effect of Shubuhuo on 30 case of Hyperplasia of M- ammary Gland after three months,including the improving of clinical symptom and the size of adenoncus of the mammary gland.The Sex hormones of lood was evaluated by radioimmunoassay,including serum estrogen(E2)、progestogen、prolactin,T-cell tubgroup(CD3、CD4) was evaluated by flow cytometry,to find the diffence between pretherapy and post-treatment in luteal.

运用疏补活汤治疗30例乳腺增生病患者3个月(1个疗程)后，观察其临床疗效，包括治疗前后患者临床症状的改善以及乳腺近红外线扫描确定乳房肿块大小的变化；在治疗前后于患者黄体期用放射免疫测定法检测血清性激素水平的变化，包括雌二醇（estrogen，E2）、孕激素（progestogen，P）、泌乳素（prolactin，PRL），同时用流式细胞仪检测治疗前后细胞免疫功能（T细胞亚群CD3、CD4）的变化。

Methods The constriction induced by norepinephine (NE, 10-8-10-4 mol/ L) and diastole induced by acetylcholine (Ach, 10-8 -10-4 mol/ L) in thoracic aortic ring with angiosteosis induced by vitamin D3 plus nicotine in rats were recorded by BL-Newcentrary multichannel physiologic recorder.

维生素D3(VitD3)和尼古丁诱导大鼠血管钙化模型，通过BL-Newcentrary型多导生理记录仪，观察钙化大鼠离体胸主动脉环对去甲肾上腺素(norepinephine，NE)(10-8～10-4mol/L)和乙酰胆碱(acetylcholine，ACh)(10-8～10-4mol/L)引起的收缩和舒张反应。

Surface morphology, microstructure and wear morphology of the coatings were investigated using model KYKY-2800 scanning electron microscope modeled. Phases of the coatings were analyzed using model D/max-RA X-ray diffractometer. Microhardness of the coatings was tested using model MH-6 hardness-testing device. The wear-resisting property of coatings was compared with 65Mn steel treated by the quenching and backfiring technology using the home-made abrasion machine. The feasibility was analyzed for reactive electric-spark build-up welding TiN cermet coating to be used for gear shaft restoration.

采用KYKY-2800型扫描电镜观察了堆焊层表面形貌、显微组织和磨损形貌，采用D/max-RA型X 射线衍射仪分析了堆焊层物相，采用MH-6型显微硬度计测定了堆焊层显微硬度，采用自制磨损实验机对比了堆焊层与淬火回火65 Mn钢的磨损性能，分析了电火花堆焊TiN金属陶瓷层用于轴颈修复的可行性。

Methods: The corneal thickness was measured before and 1,3,6,12 and 24 months after PRK with an DGHA biometer; the grades of Haze after PRK were examined with a slit-lamp biomicroscope.

采用美国DGHA型生物测量仪测量PRK术前及术后1、3、6、12与24月的角膜中心厚度，显微裂隙灯下观察PRK术后角膜雾状混浊的情况。

Methods 34 heroin addicts were equally divided into treatment group and control group.Based on substitution of Methadone decreasingly,the patients of treatment group were additionally treated with aerosl bioelectricity apparatus(which generates negative charge with 1×107/cm3),once a day,for 7 days.Each patient was inhaled negative charge for 120min.

对海洛因依赖者34例分为两组，在美沙酮替代递减治疗的同时，治疗组采用珠海产SQ-365型气溶胶生物电理疗仪，以发生1×107个/cm3的负电荷吸入120 min，每天1次，治疗7 d 后观察血、尿吗啡含量及临床症状。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。