观察仪

To 7 days after transduction, cells were observed and recorded with fluorescence microscope, the expression levels of report gene EGFP in tumor cells were examined by using flow cytometry and Western blotting, the expression of report genes luciferase was analyzed with luminometer to obtain the relative light units.

用荧光显微镜观察EGFP阳性细胞，流式细胞仪检测EGFP阳性细胞的百分率和单个细胞平均荧光强度，Western印迹检测EGFP蛋白表达的变化以比较各组病毒转导效率。

Classification examination was put into practice for only normal cells observed.For the dubious specimen,tuberculosis-Ab,acid-fast bacteria staining,flow cytometry,CD series,gene,DNA aneuploidy,ZPP and tumor markers etc were detected. Results Tuberculosis lymph gland inflammation coverd highest rate:546 cases (36%) in tuberculosis node was 252 cases (46.2%),lymphonode period was 184 cases (33.7%),early tuberculosis lymphagland inflammation was 233 cases (44.7%).

利用改良一次性5～10ml注射器(22G、7号注射器，外径＜0.7mm)，针吸取材、涂片、行改良瑞-姬双染技术，低倍镜查找特殊异常细胞，油镜仔细观察细胞形态结构特征，对于未见异常细胞要进行分类检查，对疑难标本，同时做结核抗体、抗酸菌染色、流式细胞仪、CD系列、基因、DNA异倍体、锌原卟啉、肿瘤标志物等检测指标。

Classification examination was put into practice for only normal cells observed.For the dubious specimen,tuberculosis-Ab,acid-fast bacteria staining,flow cytometry,CD series,gene,DNA aneuploidy,ZPP and tumor markers etc were detected. Results Tuberculosis lymph gland inflammation coverd highest rate:546 cases (36%) in which tuberculosis node was 252 cases (46.2%),lymphonode period was 184 cases (33.7%),early tuberculosis lymphagland inflammation was 233 cases (44.7%).

利用改良一次性5～10ml注射器(22G、7号注射器，外径＜0.7mm)，针吸取材、涂片、行改良瑞-姬双染技术，低倍镜查找特殊异常细胞，油镜仔细观察细胞形态结构特征，对于未见异常细胞要进行分类检查，对疑难标本，同时做结核抗体、抗酸菌染色、流式细胞仪、CD系列、基因、DNA异倍体、锌原卟啉、肿瘤标志物等检测指标。

By using manometric recording technique and radioactive-immunon-assay methods,the pressure in pyloric sphincter and the contents of calcitonin gen...

采用胃压测量仪监测犬幽门括约肌压力的变化，同步测定血浆及胃粘膜组织中CGRP、ET含量，并观察变化规律。

Retaining manometric tube in radial artery and polysomnography were done synchronously in 13 patients with OSAS in whom was diagnosed by whole-night PSG.

13例OSAS患者在桡动脉内留置导管监测血压并同步进行夜间睡眠多导生理仪连续记录，部分患者观察吸氧或经鼻罩持续正压通气的治疗效果。

Methods OS732 was treated with 10^(-5)MATRA for 6 days, then we observed cell morphology, counted cell number, analyzed cell cycles by flow cytometry and detected mRNA expressions of cyclinD (Dl, D2, D3), CDK4, P21 by semi-quantity RT-PCR. Treatmed with 10^(-5)MATRA, The nucleus of OS732 cells became small and condensed, and cytoplasms expanded.

用10^(-5)MATRA作用骨肉瘤细胞OS732， 6d后，进行细胞形态学观察、活细胞计数、流式细胞仪分析细胞周期、半定量RT-PCR检测细胞周期调控蛋白CyclinD(D1、D2、D3)、CDK4、P21 mRNA表达的改变。

The thermal properties and mechanical properties were analyzed by Thermogravimetric analysis and Hardness Pencil Test and Universal Test Machine, respectively. The structure of prepared PI-MWNTs was characterized by Fourier transform infrared spectrometry. The morphology of PI-MWNTs were observed by scanning electron microscopy and transmission electron microscopy. The electrical conductivity was analyzed by Super Megohmmeter.

本研究中使用能量分散光谱仪来鉴定多壁奈米碳管纯化效果，热重损失分析仪、铅笔硬度计、万能试验机分别测定材料的热稳定性质和机械性质，傅立叶转换红外光谱仪来鉴定其化学结构，扫描式电子显微镜与穿透式电子显微镜来观察材料的剖面型态与奈米碳管和基材之间的分散性，超绝缘量测仪量测材料的电性。

The diameter and electric charge of surface were determined by granularity mensuration instrument.

用显微镜法观察其形态，粒度测定仪测量粒径和表面电荷。

Methods The microstructure and components of the synthesized coatings were studied by means of X-ray diffraction and electron probe microanalysis.

利用X射线衍射仪和电子探针分析仪对涂层进行相分析、组织观察和成分分析。

By the test of clonal growth in soft agar medium , we compared the ability of cloning and proliferating among these three kinds of cells. The cell cycle was analyzed by FCM and morphosis of cells was observed under transmission eletron microscope.

通过细胞生长曲线，比较卵巢癌细胞系HO－8910与8910－p93、8910一pCDNA3.1（转染空载体ncDNA3.l）的增殖情况；通过软琼脂克隆形成试验比较这3种细胞的克隆形成能力和增殖能力；利用流式细胞仪检测这3种细胞的细胞周期的改变情况：利用透射电镜观察转染p93基因的细胞的超微结构变化；利用裸鼠移植瘤实验比较HO七与8910个93在动物体内生长状况。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。