观察

Morphology of the EPCs was observed, and the growth curve of the EPCs was investigated. Surface antigens of the EPCs were analyzed by the flow-cytometer. The capability of intaking the acetylated low-density lipoprotein of the EPCs was detected using fluoresencent chemical method. The vasoformative capability and genetic stability of EPCs were cultured in matrigel, and examined by karyotype analysis.

观察培养细胞的形态，测定其生长动力学，采用流式细胞仪及免疫细胞化学方法检测其表型，应用细胞荧光化学法分析EPCs对乙酰化低密度脂蛋白(acetylated low-density lipoprotein， ac-LDL)的摄取功能，Matrigel上培养并检测其形成血管能力，染色体核型分析其遗传稳定性，裸鼠实验、软琼脂实验观察致瘤性。

After consented and investigated using questionnaire (including risk factors of cervical cancer, knowledge of cancers), eligible women were examined by VIA and VILI and women who had ab...

对符合条件的妇女进行危险因素、癌症及子宫颈癌认知情况的问卷调查，用醋酸染色后肉眼观察和碘染色后肉眼观察进行宫颈检查，结果异常者进行阴道镜下活组织检查并得到最终的病理学诊断。

Cytokines found to have up-regulated in cells were measured in serum and lung tissue of rats exposed to large tidal volume ventilation. For investigating the intracellular pathway of cytokine up-regulation in VILI, exogenous TNF-α or H2O2 was added to culture media of alveolar epithelial cells. Cytokines were then measured. To explore the role of oxidant stress in VILI, N-acetylcysteine, as an anti-oxidant, was used in vitro and in vivo.

我们在体外培养的肺上皮细胞中加入外源性肿瘤坏死因子、过氧化氢或N-乙酰半胱氨酸，观察细胞因子的变化。N-乙酰半胱氨酸亦被投入大鼠呼吸机诱导肺损伤模型，观察用药后细胞因子的变化。

The iso-electric point of was 4.8-5.2. The amino acid analysis indicated that the recombinant protein is non- N-glycosylated.The ability of 0.5 ug/m rhsTRAIL to induce apoptosis of liver cancer cells SMMC7721 and BEL 7402 could be visioned morphologically. This was further verified by Electrical microscope observision of induced SMMC7721 cells and agarose gel electrophoresis of apoptosis DNA. MTT test results also showed that 0.01 u g/ml purified rhsTRAIL has strong effect to induce apoptosis of SMMC7721 cell.

形态学上观察了rhsTRAIL对肝癌细胞SMMC7721和BEL7402的诱导凋亡活性，发现rhsTRAIL在0.5μg/ml时显示了很好的诱导凋亡活性，并通过电镜观察rhsTRAIL诱导后的SMMC7721细胞，证实了凋亡；同时提取凋亡细胞DNA，进行琼脂糖电泳分析，在分子水平证明凋亡；最后利用SMMC7721细胞分组加入纯化后的rhsTRAIL蛋白，结果显示纯化蛋白含量在0.01μg/ml时细胞就呈现了诱导凋亡活性。

MATERAIL AND METHODS: The cell proliferation inhibition was measured by (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromid,MTT) colorimetric assay. Morphological changes of EJ cells were observed by fluorescence staining of Hoechst 33258. Cell cycle and apoptosis were analyzed by flow cytometry.

材料与方法：四甲基偶氮唑蓝(3-[4，5-dimethylthiazol-2-yl]-2，5 diphenyl tetrazolium bromid，MTT)法检测EJ细胞增殖活力；荧光染色观察凋亡细胞的形态学变化；流式细胞术分析细胞周期及细胞凋亡；免疫细胞化学染色观察caspase-3 蛋白的表达。

Rats were treated with Mangan Misture,and contrasted with those accepted hepatic cells generating factor.

方法采用大鼠部分肝切除法复制肝细胞再生模型，给予慢肝合剂，并与促肝细胞生长因子治疗者对比，同时计算肝指数的变化，采用光镜观察肝脏HE的变化及用电镜观察肝脏超微结构的变化。

METHODS Low-dose Peth 0.01, 0.1 and 1 mgkg^(-1) were given iv 10 min before guinea pigs inhaled 75% cigarette smoke 60 mL. Then, the changes in airway resistance R(subscript aw, dynamic lung compliance C(subscript dyn and vascular permeability in airway tissues were observed. The total leukocyte and the differential leukocyte in bronchoalveolar lavage fluid and nitric oxid e content in BALF were investigated after guinea pigs given iv Peth 0.1 mgkg^(-1) 10 min before cigarette smoke inhalation and consecutively inhaled 360 mL smoke in 2 h.

观察Peth 0.01，0.1和1mgkg^(-1)对豚鼠自主吸入75%香烟烟雾（含25% O2）60mL后，气道阻力和肺动态顺应性变化的影响及气道组织血管通透性变化的影响；观察Peth 0.1mgkg^(-1)对豚鼠2h内分6次吸入（共360mL）75%浓度的香烟烟雾后，支气管肺泡灌洗液中白细胞总数和分类计数改变的影响，测定BALF中一氧化氮含量。

Methods The spontaneous contraction of gastric smooth muscle strip was recorded by using physiograph in rats. The change of tension in response to different concentrations of CNP and sodium nitroprusside, co-incubated with high concentration glucose (30 mmol/L) or mannitol were detected.

取大鼠40只制备胃窦环形肌条，肌条收缩活动稳定后，对照1、2组分别加入不同浓度CNP1×10^(-8、3×10^(-8)、1×10^(-7)mol/L及硝普钠1×10^(-7、3×10^(-7)、1×10^(-6)mol/L，观察其效应，每次加药观察20 min，冲洗3次；高糖1、2组预加葡萄糖使浴槽终浓度为30 mmol/L，其后操作同对照1、2组；甘露醇组预加甘露醇30mmol/L，其后操作同对照1组；均采用多道生理信号采集处理系统计算CNP致平滑肌张力的抑制率。

.to record the bp and hr15,30,45,60min in both groups.bp,hr were higher during anesthesia than before anesthesia significantly in control group;bp,hr were lower during anesthesia than before anesthesia significantly in observation group.discussion remifentail take from the bp,hr effectively during operation,reduce the blooding,ensure the operative field clean and maintain the hemodynamics tranquilization,so as to the operation go with a swing.

。观察记录手术前及手术开始后5，15，30，45min时患者收缩压及心率。与麻醉前比较，对照组患者麻醉期间收缩压及心率均明显升高，而观察组患者均显著下降，两者间差异有统计学意义。［结论］瑞芬太尼可有效地降低手术期间患者的血压及心率，减少手术视野出血，保持血液动力学指标稳定。

What we have to do is to be for ever curiously testing new opinions and courting new impressions, never acquiescing in a facile orthodoxy of Comet, or of Hegel, or of our own .

感到了人生经验的五色缤纷及倏忽无常，我们拼出全部力气进行观察和接触，哪里还有时间去为自己观察和接触到的事物制订出一套一套的理论？

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。